The hatched chick embryos were collected in centrifuge tubes after 4.5 days, fixed with a fixative, and dehydrated after 24 h of fixation. The embryos were then placed in xylene I for transparency for 15 min and xylene II for transparency for 15 min. The transparent chicken embryos were soaked in wax at 65°C for 1 h and placed in a preheated embedding frame, and hot wax was poured into the embedding frame. Preheated tweezers were used to center the chicken embryos and remove air bubbles. After the wax was completely solidified, it was removed and trimmed, and the trimmed wax block was placed on a microtome for slicing. The slices were displayed in a 40% alcohol aqueous solution, and the slices were placed in warm water at 42°C for 1-2 s before the sections were attached to the slides. The slides were placed in a slide holder at 65°C to bake overnight, and the slides were deparaffinized. After deparaffinization and rehydration, the sections were air-dried and processed using a Periodic Acid Schiff Staining Kit (Solarbio, Item No: G1281).
Periodic acid schiff staining kit
Manufactured by Solarbio
Sourced in China
The Periodic Acid Schiff (PAS) Staining Kit is a laboratory reagent used for the detection and visualization of carbohydrates, glycoproteins, and mucopolysaccharides in tissue samples. The kit includes all the necessary components for performing the PAS staining procedure.
Automatically generated - may contain errors
2 protocols using periodic acid schiff staining kit
1
Histological Preparation of Chick Embryos
2
Histological Assessment of Tubular Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin sectioning was performed at 4 μM. After dewaxing in xylene and rehydration in gradient ethanol, hematoxylin-eosin staining (HE staining) was conducted with an HE staining kit (Beyotime, China, Cat# C0105). Periodic acid-Schiff staining (PAS) was performed with a periodic acid-Schiff staining kit (Solarbio, China, Cat# G1281). Masson’s trichrome staining was performed with a kit from Nanjing Jiancheng Bio., China (Cat# D026). The above staining procedure were done according to the manufacturer’s instructions. Tubular injury score was semiquantitatively calculated based on PAS staining according to the percentage of cortical tubular necrosis with an assigned value: 0, none; 1, 10%; 2, 10% to 25%; 3, 25% to 75%; and 4, >75% [16 (link)]. Immunohistochemical staining (IHC) was performed with Biotin-Streptavidin HRP-based SPlink Detection Kits (ZSGB-Bio, China, Cat# SP-9002) and all procedures followed the manufacturer’s instructions. Antibodies used in IHC were mouse anti-α-SMA (1: 100, Boster, China, Cat# BM0002) and mouse anti-fibronectin (1: 100, DHSB, USA, Cat# P1H11). Images were taken with a light microscope (Nikon Eclipse 80i, Japan) at 200× magnification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!