Cxcl10

Western blots were performed according to standard protocols. A 12% SDS-PAGE gel was used to separate total proteins extracted from mice colon tissue. Then, proteins were transferred onto polyvinylidene fluoride membranes. The membranes were incubated with primary antibodies for CXCL8 (Novus) and CXCL10 (Affinity) overnight at 4 °C after blocking with 5% non-fat dry milk in PBST. Anti-GAPDH (Beyotime, 1:1000) was used as the control. Each experiment has been repeated at least three times.

The following antibodies were used: RNF8 (Proteintech, # 14112-1-AP), β-tubulin (Affinity, # T0023), CD3 (Affinity, # DF6594), IL-12 (Affinity, # AF5133), IFN-γ (Affinity, # DF6045), CXCL-9 (Affinity, # DF9920), CXCL-10 (Affinity, # DF6417), galectin-3 (Proteintech, # 14979-1-AP), HIF-1α (Proteintech, # 66730-1-Ig), p-IκBα (Abcam, # ab133462), NF-κB (Abcam, # ab16502), P-NF-κB (Santacruze, # sc-101752), PCNA (Proteintech, # 60097-1-Ig), Flag (Proteintech, # 20543-1-AP), HA (Proteintech, # 66006-2-Ig), His (Proteintech, # 66005-1-Ig), ubiquitin (CST, # 91112 S), K48 (CST, # 12805), K63 (CST, # 5621S). Secondary anti-mouse (# SA00001-1), and anti-rabbit (# SA00001-2) horseradish-coupled antibodies were from Proteintech.