Anti s6

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-S6 is a primary antibody that specifically binds to the S6 ribosomal protein. S6 is a component of the 40S subunit of the eukaryotic ribosome and plays a role in protein synthesis.

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Lab products found in correlation

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Anti-p70 S6 Kinase (5 citations) Anti-phospho-S6 (3 citations) Anti-S6 ribosomal protein (2 citations) Anti-phospho-S6 ribosomal protein (Ser235/236) (2 citations)

3 protocols using anti s6

Western Blot Analysis of Tumor Tissue

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Western blot analysis was carried out according to standard procedures using enhanced-chemiluminescence detection (GE Life Science, Chicago, IL, USA). Tumor tissue lysates were prepared using an electric homogenizer for 30 seconds after the addition of lysis buffer. The following antibodies were used: anti-β-actin, anti-acetyl-CoA carboxylase (ACC), anti-phospho-ACC, anti-phospho-AKT, anti-phospho-BRCA1, anti-caspase-3, anti-caspase-7, anti-β-catenin, anti-phospho-cyclin D1, anti-phospho-GSKα/β, anti-MAPK, anti-phospho-MAPK, anti-PARP, anti-PDK1, anti-phospho-PDK1, anti-PI3K, anti-phospho-PI3K, anti-phospho-S6, anti-phospho-mTOR, anti-phospho-Rb, anti-VEGFR, anti-phospho-VEGFR (all from Cell Signaling Technology, Danvers, MA, USA); anti-β-actin, anti-AKT, anti-AKT1, anti-BRCA1, anti-cyclin D1, anti-Rb, anti-S6, anti-mTOR, anti-α-tubulin (all from Santa Cruz, Dallas, TX, USA); and anti-PCNA (Atlas Antibodies, Bromma, Sweden). Horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse antibodies (Jackson Immuno Research, West Grove, PA, USA) were used as secondary antibodies as appropriate.

Baek H.J., Kim S.E., Kim J.K., Shin D.H., Kim T.H., Kim K.G., Deng C.X, & Kim S.S. (2018). Inhibition of AKT suppresses the initiation and progression of BRCA1-associated mammary tumors. International Journal of Biological Sciences, 14(13), 1769-1781.

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Knockdown of Mouse Srsf3 Gene

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Cells were transfected with siRNAs synthesized by Integrated DNA Technologies (IDT) using RNAiMax Transfection reagents (Thermo Fisher Scientific) recommended by the manufacturer’s protocol. siRNA targeting mouse Srsf3: 5′-CGUGAUAUCAAGAAUUGU-3′. The knockdown efficiency of Srsf3 was determined by qPCR with forward primer 5′-GCTGCCGTGTAAGAGTGGAA-3′ and reverse primer 5′-AGGACTCCTCCTGCGGTAAT-3′. The antibodies used for Western blot analysis include anti-Flag (No. F3165, Sigma Aldrich, Burlington, MA, USA), anti-Actin (No. 612657 BD Biosciences, Durham, NC, USA), anti-4EBP1 (#9644, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-S6 (#4857, Cell Signaling Technology), anti-S6 (sc-74576, Santa Cruz Biotechnology, Dallas, TX, USA), anti-Tubulin (12004166, Bio-Rad, Hercules, CA, USA), Anti-Secondary antibody against mouse: goat-anti-mouse IgG-HRP (No. sc-2005, Santa Cruz Biotechnology).

Cheng S., Fahmi N.A., Park M., Sun J., Thao K., Yeh H.S., Zhang W, & Yong J. (2022). mTOR Contributes to the Proteome Diversity through Transcriptome-Wide Alternative Splicing. International Journal of Molecular Sciences, 23(20), 12416.

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Cellular Stress Induction and Detection

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To induce cellular stress, sodium arsenite (arsenite, NaAsO₂; Sigma-Aldrich) and potassium tellurite (tellurite, K₂TeO₃ kindly gifted by Dr. José Manuel Pérez-Donoso, UNAB, Chile) were used. Cycloheximide and Puromycin were obtained from Sigma Aldrich. The antibodies used in this work included the following: anti-phospho-eIF2α (rabbit polyclonal) and anti-GAPDH (mouse monoclonal) from Abcam; anti-eIF2α (mouse monoclonal), anti-phospho-S6K1 (rabbit monoclonal), anti-phospho-S6 (rabbit monoclonal), anti-phospho-4EBP1 (rabbit monoclonal) from Cell Signaling Technology; anti-Puromycin (mouse monoclonal) from Millipore; anti-DCP1a (mouse monoclonal), anti-eIF3b (mouse monoclonal), anti-TIAR (goat polyclonal), anti-DDX3 (rabbit polyclonal), anti-phospho-mTOR (mouse monoclonal), anti-mTOR (mouse monoclonal), anti-S6K1 (mouse monoclonal), anti-S6 (mouse monoclonal), anti-4EBP1 (mouse monoclonal) from Santa Cruz Biotechnology and anti-SC35 (mouse monoclonal) kindly provided by Dr. Verónica Noches, PUC, Chile. Secondary antibodies used for immunofluorescence included the following: Alexa Fluor 594 donkey anti-mouse, Alexa Fluor 594 donkey anti-rabbit and Alexa Fluor 647 donkey anti-mouse or anti-goat from Life Technologies; and secondary antibodies for western blot (horseradish peroxidase-conjugated) anti-mouse and anti-rabbit were purchased from Jackson Immunoresearch.

Gaete-Argel A., Velásquez F., Márquez C.L., Rojas-Araya B., Bueno-Nieto C., Marín-Rojas J., Cuevas-Zúñiga M., Soto-Rifo R, & Valiente-Echeverría F. (2021). Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage. Frontiers in Cell and Developmental Biology, 9, 622057.

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