Rabbit anti lc3 nb100 2220

Rabbit anti-AMPKα1/2 (sc-25792) was purchased from Santa Cruz. Rabbit anti-ULK1 (#5723-1) was purchased from Epitomics. Rabbit anti-phospho-AMPKα (Thr172) (#2535), rabbit anti-phospho-ULK1 (Ser555, #5869) (Ser757, #6888), rabbit anti-CaMKII (#4436), rabbit anti-BECN1 (#3738) and anti-mTOR (#2972) were purchased from Cell Signaling Technology. Rabbit anti-LKB1 (L7917) and rabbit anti-p62/SQSTM1 (P0067) were purchased from Sigma. Rabbit anti-LC3 (NB100-2220) was obtained from Novus Biologicals. Mouse anti-β-actin (Sr-25113) was purchased from Subrray Biotechnology. A mouse PrP-specific antibody 6D11 (sc-58581) that recognises PrP^C and PrP^Sc was purchased from Santa Cruz. Horseradish peroxidase (HRP)-conjugated goat anti-mouse or rabbit IgG (#31430 or #31460) was purchased from Thermo. Both si-RNA-ULK1 (Stealth RNAi) and si-RNA-NC were designed and synthesised by Life Technologies. DAPI (D1306) was from Invitrogen. Lysosome inhibitor NH₄Cl (A9434) was purchased from Sigma. Protease inhibitor cocktail set III was from Merck (539134) and the enhanced chemoluminescence system (ECL) was from PerkinElmer (NEL103E001EA). Lipofectamine 2000 was purchased from Life Technologies.

Genistein (G6649), Filipin (F9765), and Chloroquine (C6628) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Torin 1 (4247) was purchased from Tocris Bioscience (Minneapolis, MN, USA). U18666A (BML-S200) was purchased from Enzo Life Sciences Inc. (Farmingdale, NY, USA). The β-Hexosaminidase substrate 4-methylumbelliferyl N-acetyl-β-d-glucosaminide (M2133) was purchased from Sigma–Aldrich (St. Louis, MO, USA). Antibodies for rabbit anti-TFEB (4240) and rabbit anti-Histone H3 (9715) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Mouse anti-GAPDH (Human Glyceraldehyde-3-phosphate dehydrogenase) (sc-32233) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit anti-LC3 (NB100-2220) was purchased from Novus Biologicals. Rabbit anti-p62 (56416) was purchased from Abcam (Cambridge, UK).