Immunostaining was performed with antigen retrieval (20–30′ in boiling 10mM sodium citrate pH 6.0, 0.05% Tween 20) and by incubating primary antibodies overnight at 4°C. Detection and staining was performed with Vectastain ABC kit and DAB kits (Vector laboratories). Slides were counterstained with Hematoxylin. Antibodies used: anti-APC #sc-896 and anti-AR #sc-816, from Santa Cruz Technology; anti-β-catenin #610181 (BD); anti-chromogranin A #ab1560-1, anti-MLH1 #ab92312, anti-Sox9 # ab5535, and anti-P-MEK1 ab59329 from Abcam; anti-ck17/19 #3984, anti-MSH2 #D24B5, and anti-γ-H2AX #9718, from Cell Signaling; anti-p21 #LS-C88585 (Lifespan Biosciences), and anti-p53 #CM5 (Vector Laboratories).
Anti p mek1 ab59329
Manufactured by Abcam
Anti-P-MEK1 ab59329 is a primary antibody that recognizes the phosphorylated form of MEK1, a key enzyme in the MAPK signaling pathway. This antibody can be used to detect and quantify the activated state of MEK1 in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti apc sc 896, by Santa Cruz Biotechnology (2 mentions)
Anti β catenin 610181, by BD (2 mentions)
Anti chromogranin a ab1560 1, by Abcam (2 mentions)
Anti mlh1 ab92312, by Abcam (2 mentions)
Anti sox9 ab5535, by Abcam (2 mentions)
Anti ck17 19 3984, by Cell Signaling Technology (2 mentions)
Anti msh2 d24b5, by Cell Signaling Technology (2 mentions)
Anti p21 ls c88585, by LifeSpan BioSciences (2 mentions)
Dab kit, by Vector Laboratories (2 mentions)
Anti ar sc 816, by Santa Cruz Biotechnology (2 mentions)
Anti p53 cm5, by Vector Laboratories (2 mentions)
Anti γh2ax, by Cell Signaling Technology (2 mentions)
Vectastain abc kit, by Vector Laboratories (2 mentions)
2 protocols using anti p mek1 ab59329
1
Immunostaining Protocol with Antigen Retrieval
2
Immunostaining Protocol with Antigen Retrieval
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Immunostaining was performed with antigen retrieval (20–30′ in boiling 10mM sodium citrate pH 6.0, 0.05% Tween 20) and by incubating primary antibodies overnight at 4°C. Detection and staining was performed with Vectastain ABC kit and DAB kits (Vector laboratories). Slides were counterstained with Hematoxylin. Antibodies used: anti-APC #sc-896 and anti-AR #sc-816, from Santa Cruz Technology; anti-β-catenin #610181 (BD); anti-chromogranin A #ab1560-1, anti-MLH1 #ab92312, anti-Sox9 # ab5535, and anti-P-MEK1 ab59329 from Abcam; anti-ck17/19 #3984, anti-MSH2 #D24B5, and anti-γ-H2AX #9718, from Cell Signaling; anti-p21 #LS-C88585 (Lifespan Biosciences), and anti-p53 #CM5 (Vector Laboratories).
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