The total protein was extracted from the mouse colon tissue according to the method of Wang et al. [22] (link). The protein concentration was measured by the bicinchoninic acid (BCA) assay, and the same concentration was used in all subsequent experiments. After electrophoresis, the sample was transferred to a membrane made of polyvinylidene fluoride (PVDF). These were then blocked with 3% bovine serum albumin (BSA) at 25 °C for 1 h. The rabbit antibodies used included anti-β-actin (Solarbio, China), anti-TLR4 (No. bs-20595R; Bioss Antibodies Inc., Woburn, MA, USA), anti-MyD88 (No. GTX112987; GeneTex In c., Irvine, CA, USA), anti-PI3K (No. ab40776; Abcam, Cambridge, UK), anti-p-PI3K (No. ab278545; Abcam), anti-Akt (No. N3C2; GeneTex Inc.), anti-Akt (No. GTX128414; Gene-Tex Inc.), anti-NF-κB (No. ab16502; Abcam), and anti-IκBα (No. ab32518; Abcam). The membrane was incubated at 4 °C overnight, subjected to horseradish peroxidase (HRP)-coupled secondary antibody, and kept in the dark for 1 h. The bands were detected with Cytiva ImageQuant™ LAS 4000 (Cytiva Life Sciences, Marlborough, MA, USA), and the proteins were expressed as a percentage of β-actin.
Anti myd88
Manufactured by GeneTex
Sourced in United States
Anti-MyD88 is a primary antibody that specifically targets the MyD88 protein. MyD88 is an adaptor protein involved in the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. The Anti-MyD88 antibody is used for the detection and analysis of MyD88 expression in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Solarbio (1 mentions)
Anti akt, by GeneTex (1 mentions)
Anti iκbα, by Abcam (1 mentions)
Anti nf κb, by Abcam (1 mentions)
Anti p pi3k, by Abcam (1 mentions)
Anti pi3k, by Abcam (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by GeneTex (1 mentions)
Anti gapdh, by GeneTex (1 mentions)
2 protocols using anti myd88
1
Protein Extraction and Western Blot Analysis from Mouse Colon
2
Western Blot Analysis of MyD88 and NF-κB
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After lysis, lung tissue samples (50 μg) were run on 12.5% sodium dodecyl sulfate polyacrylamide gels and transferred to polyvinylidene fluoride membranes. After blocking with 5% bovine serum albumin in Tris-buffered saline, the membranes were incubated overnight at 4 °C with primary antibodies, anti-MyD88 (Gene Tex, GTX112987), or anti-phospho-NFκB (Gene Tex, GTX50254). Then, the membranes reacted with horseradish peroxidase-conjugated secondary antibody (Gene Tex, GTX213110-01) at room temperature for 1 h. As a loading control, the same blots were re-probed with anti-GAPDH (Gene Tex, GTX100118) and anti-mouse horseradish peroxidase antibodies. All samples were performed in triplicate.
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