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Thermo scientific pepmap c18 50 cm x 75 mm id column

Manufactured by Thermo Fisher Scientific
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The Thermo Scientific PepMap C18 50 cm x 75 mm ID column is designed for reversed-phase liquid chromatography (RPLC) separation of peptides and proteins. It features a porous silica particle with a C18 bonded phase.

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Lab products found in correlation

Thermo scientific ultimate 3000 rslcnano system, by Thermo Fisher Scientific (2 mentions) Q exactive hf, by Thermo Fisher Scientific (1 mentions) Orbitrap fusion lumos mass spectrometer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Thermo Scientific C18 column PepMap C18 column Thermo C18 column PepMap C18 C18 column

2 protocols using thermo scientific pepmap c18 50 cm x 75 mm id column

1

Comprehensive Proteomic Analysis by LC-MS/MS

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For proteome analysis, LC-MS/MS analysis was performed with a Thermo Scientific UltiMate 3000 RSLCnano system (Thermo Fisher Scientific) coupled to a Q Exactive HF (Thermo Fisher Scientific). The fractionated peptides were separated using Thermo Scientific PepMap C18 50 cm x 75 mm ID column (Thermo Fisher Scientific) with a 5%-28% ACN gradient in 0.1% FA over 200 min at a flow rate of 250 nL/min. The spectra of full MS scan (m/z 375-1600) were acquired in the Orbitrap mass analyzer at 120,000 resolution for a maximum injection time of 50 ms with an AGC target value of 3e6. Up to 15 precursors were selected for MS2 analysis with an isolation window of 0.7 Th and dynamic exclusion time was set to 20 s. Precursors were fragmented by HCD using a normalized collision energy of 33% and analyzed using the Orbitrap at 60,000 resolution for a maximum injection time of 120 ms with AGC target value of 1e5. Precursor ions with unassigned charge state as well as charge state of 1+, or superior to 7+ were excluded from fragmentation selection.
Chen Y.J., Roumeliotis T.I., Chang Y.H., Chen C.T., Han C.L., Lin M.H., Chen H.W., Chang G.C., Chang Y.L., Wu C.T., Lin M.W., Hsieh M.S., Wang Y.T., Chen Y.R., Jonassen I., Ghavidel F.Z., Lin Z.S., Lin K.T., Chen C.W., Sheu P.Y., Hung C.T., Huang K.C., Yang H.C., Lin P.Y., Yen T.C., Lin Y.W., Wang J.H., Raghav L., Lin C.Y., Chen Y.S., Wu P.S., Lai C.T., Weng S.H., Su K.Y., Chang W.H., Tsai P.Y., Robles A.I., Rodriguez H., Hsiao Y.J., Chang W.H., Sung T.Y., Chen J.S., Yu S.L., Choudhary J.S., Chen H.Y., Yang P.C, & Chen Y.J. (2020). Proteogenomics of Non-smoking Lung Cancer in East Asia Delineates Molecular Signatures of Pathogenesis and Progression. Cell, 182(1).
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2

Phosphoproteome Analysis by LC-MS/MS

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For phosphoproteome analysis, LC-MS/MS analysis was performed with a Thermo Scientific UltiMate 3000 RSLCnano system (Thermo Fisher Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Enriched phosphopeptides were dissolved in 1% formic acid and separated using Thermo Scientific PepMap C18 50 cm x 75 mm ID column (Thermo Fisher Scientific) with a 5%-28% ACN gradient in 0.1% FA over 200 min at a flow rate of 250 nL/min. The full MS spectra (m/z 375-1,600) were acquired in Orbitrap at 120,000 resolution with an AGC target value of 4e5 charges for a maximum injection time of 50 ms. Precursors for MS2 analysis were selected using a Top10 method with an isolation window of 0.7 Th. MS2 precursors were fragmented by HCD using a normalized collision energy of 36%. The AGC target value was set to 5e4 and the dynamic exclusion was set to 20 s. MS2 spectra were acquired in Orbitrap with a maximum injection time of 150 ms at a resolution of 60,000. Precursor ions with the charge state of unassigned, 1+, or superior to 7+ were excluded from fragmentation selection.
Chen Y.J., Roumeliotis T.I., Chang Y.H., Chen C.T., Han C.L., Lin M.H., Chen H.W., Chang G.C., Chang Y.L., Wu C.T., Lin M.W., Hsieh M.S., Wang Y.T., Chen Y.R., Jonassen I., Ghavidel F.Z., Lin Z.S., Lin K.T., Chen C.W., Sheu P.Y., Hung C.T., Huang K.C., Yang H.C., Lin P.Y., Yen T.C., Lin Y.W., Wang J.H., Raghav L., Lin C.Y., Chen Y.S., Wu P.S., Lai C.T., Weng S.H., Su K.Y., Chang W.H., Tsai P.Y., Robles A.I., Rodriguez H., Hsiao Y.J., Chang W.H., Sung T.Y., Chen J.S., Yu S.L., Choudhary J.S., Chen H.Y., Yang P.C, & Chen Y.J. (2020). Proteogenomics of Non-smoking Lung Cancer in East Asia Delineates Molecular Signatures of Pathogenesis and Progression. Cell, 182(1).
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