D8740

Human colon cancer cell line: SW620, CW-2, LoVo and HCT116 purchased from ATCC (90% DMEM + 10% FBS) 0.1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000(ammonium salt) (DSPE-PEG2000-NH2) were purchased from Ananti Polar Lipids, Inc. (Beijing, China). Cetuximab, a monoclonal antibody anti-EGFR, was purchased from Shanghai TheraMabsBiotech (product number TM-Beva-00002). DMSO (D8371, Solarbio), dialysis bag (3.5KD, F132590, BBI), DMEM medium (Cat. No. 11965-126), fetal bovine serum (FBS) (product number 16000-044), trypsin and double antibodies were purchased from Gibco company. MTT powder was purchased from Azerbaijan Latin (Cat. No. T100896-1g), irinotecan (Irinotecan, CPT-11), purchased from Solarbio (SI8480-20 mg), EDC purchased from Sigma (D8740-500 mg), NHS purchased from Sigma (D8740-500 mg) Balb/c nude mice were purchased from Weitong Lihua.
Main instruments: ordinary microscope (OLYMPUS company in Japan), small desktop refrigerated centrifuge (Technology University Zhongjia), cell incubator (American Thermoelectric), heating magnetic stirrer (C-MAG HS 10, IKA) CM-H₂DCFDA were obtained from ThermoFisher.