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Culture flasks and cryotubes

Manufactured by Avantor
Sourced in Norway

Culture flasks and cryotubes are laboratory equipment used for cell and tissue culture applications. Culture flasks provide a sterile, controlled environment for cultivating cells, while cryotubes are used for the storage and preservation of biological samples at cryogenic temperatures.

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2 protocols using culture flasks and cryotubes

1

Detailed Reagent and Equipment Specifications

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Chemicals and reagents were purchased from Merck KGaA (Darmstadt, Germany) unless otherwise stated, and were of analytical grade. Culture flasks and cryotubes were purchased from VWR (Oslo, Norway), centrifuge tubes from Sarstedt (Oslo, Norway) and Petaka® G3 LOT (Celartia, Columbus, OH, USA) hypoxic cell culture bioreactors (hereafter entitled “Petakas”) from Tebu-Bio (Roskilde, Denmark). Horse serum and sodium pyruvate were obtained from Thermo Fisher Scientific (Oslo, Norway) and tetrahydrouridine (THU) from AH diagnostics (Oslo, Norway). Reagents and equipment used for liquid chromatography tandem mass spectrometric methods (LC-MS/MS) are described elsewhere [24 (link),25 (link)].
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2

Reagent and Equipment Sources Documented

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Unless otherwise stated, chemicals and reagents were purchased from Merck KGaA (Darmstadt, Germany) and were of analytical grade. Horse serum and sodium pyruvate were bought from Thermo Fisher Scientific (Oslo, Norway), culture flasks and cryotubes from VWR (Oslo, Norway), centrifuge tubes from Sarstedt (Oslo, Norway), and THU from AH diagnostics (Oslo, Norway). All other reagents and equipment used for liquid chromatography tandem mass spectrometry methods have been described previously (Bjånes et al., 2015 (link); Kamčeva et al., 2015 ).
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