Rabbit monoclonal anti ppar γ

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit monoclonal anti-PPAR-γ is a primary antibody that specifically recognizes the peroxisome proliferator-activated receptor gamma (PPAR-γ) protein. PPAR-γ is a nuclear receptor that plays a key role in the regulation of adipocyte differentiation and glucose and lipid metabolism.

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Lab products found in correlation

Rabbit monoclonal anti tgfβ, by Abcam (1 mentions) Rabbit monoclonal anti il 6, by Cell Signaling Technology (1 mentions) Rabbit polyclonal anti mmp 9, by Abcam (1 mentions) Eclipse 80i, by Nikon (1 mentions) Non immunized rabbit igg, by Thermo Fisher Scientific (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Anti tgf β1, by Santa Cruz Biotechnology (1 mentions) Vb 7000, by Keyence (1 mentions)

Spelling variants of this product

Monoclonal rabbit (3 citations) Anti-rabbit PPAR-γ (2 citations) Anti-PPAR (2 citations)

2 protocols using rabbit monoclonal anti ppar γ

Comprehensive Protein Analysis Protocol

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Protein was extracted by RIPA buffer containing protease and phosphatase inhibitors. 6-10% Bis-Tris protein gels were equally loaded with 30μg protein, electrophoresed at 110 V, and electro-transferred to PVDF membranes. Membranes were blocked in 10% milk/TBST and immunoblotted with the following antibodies: rabbit polyclonal anti-MMP-9 (Abcam, 1:1000), rabbit monoclonal anti-TGF-β (Abcam, 1:1000), rabbit polyclonal anti-ARG1 (Abcam, 1:1000), rabbit monoclonal anti-VEGF-A (Abcam, 1:1000), rabbit monoclonal anti-S100A8 (Abcam, 1:1000), rabbit monoclonal anti-TNF-α (Abcam, 1:1000), rabbit monoclonal anti-IL-6 (Cell Signaling Technology, 1:1000), rabbit polyclonal anti-DNMT1 (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-EGR1(Abcam, 1:1000), rabbit monoclonal anti-EGR2 (Abcam, 1:1000), rabbit monoclonal anti-PPAR-γ (Cell Signaling Technology, 1:1000), mouse monoclonal anti-Maf-b (Santa Cruz Biotechnology, 1:100), rabbit monoclonal anti-p50 (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-p52 (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-Rel-B (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-p65 (Cell Signaling Technology, 1:1000), mouse monoclonal antî-Actin (Sigma Aldrich, 1:10000). The loading control antibodies (ant-β-Actin) in all cases were applied. Information about all the antibodies used is provided in Supplementary Table 3.

Lu Z., Zou J., Li S., Topper M.J., Tao Y., Zhang H., Jiao X., Xie W., Kong X., Vaz M., Li H., Cai Y., Xia L., Huang P., Rodgers K., Lee B., Riemer J.B., Day C.P., Yen R.W., Cui Y., Wang Y., Wang Y., Zhang W., Easwaran H., Hulbert A., Kim K., Juergens R.A., Yang S.C., Battafarano R.J., Bush E.L., Broderick S.R., Cattaneo S.M., Brahmer J.R., Rudin C.M., Wrangle J., Mei Y., Kim Y.J., Zhang B., Wang K.K., Forde P.M., Margolick J.B., Nelkin B.D., Zahnow C.A., Pardoll D.M., Housseau F., Baylin S.B., Shen L, & Brock M.V. (2020). Epigenetic Therapy Inhibits Metastases by Disrupting Premetastatic Niches. Nature, 579(7798), 284-290.

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Immunofluorescent Characterization of Platelet Proteins

Cited 1 time

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Fixed platelets on the cp-Ti plates were directly subjected to treatment with primary antibodies, such as mouse monoclonal anti-CD62P antibody (1:20 dilution; BioLegend, San Diego, CA, USA), rabbit polyclonal anti-PDGF-B (1:200 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-TGFβ1 (1:200 dilution; Santa Cruz Biotechnology), rabbit monoclonal anti-PPARγ (1:100 dilution; Cell Signaling Technology, Danvers, MA, USA), and mouse monoclonal anti-fibrin antibodies (1:400 dilution; GeneTex, Inc., Irvine, CA, USA) overnight at 4 °C. In this study, based on our preliminary observations that our routine blocking or washing with Tween-20-containing PBS also visualized intra-platelet binding of the antibodies we used, we did not perform blocking or use such a detergent-containing PBS in the main experiments in this study.
The samples were then probed with the corresponding secondary antibodies or non-immunized rabbit IgG (Life Technologies Corporation, Carlsbad, CA, USA) as an isotype control for 60 min at ambient temperature in the dark. The samples were finally mounted using an antifade mounting medium (Vectashield; Vector Laboratories, Burlingame, CA, USA) and examined under a fluorescence microscope (ECLIPSE 80i; Nikon) connected with a cooled CCD camera (VB-7000; Keyence) [23 (link)].

Tsujino T., Takahashi A., Watanabe T., Isobe K., Kitamura Y., Okuda K., Nakata K, & Kawase T. (2019). Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets. Dentistry Journal, 7(4), 109.

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