Sulforhodamine b

In this study, we work on human MCF7 breast cancer cells (ATCC® HTB-22), human PC3 prostate cancer cell line (ATCC® CRL-1435), A375 human skin cancer cell line (ATCC® CRL-1619), PANC1 pancreatic cell line (ATCC® CRL-1469), A549 lung cancer cell line (ATCC® CCL-185) and colorectal cancer cell line CACO2 (ATCC HTB-37).[33 (link)] All of the cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose containing 10% Fetal Bovine Serum (FBS) (Bio Whittaker, Verviers, Belgium), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Buffer (10 mM), gentamicin (50 μg/mL), L-glutamine (100 μg/mL), streptomycin (100 μg/mL), penicillin (100 μg/mL), (Sigma, St. Luis, MO, USA). Trypsin-EDTA (Biowest, USA) was routinely used for subcultures. Cell growth was accomplished at 37°C in a 5% carbon dioxide 95% air atmosphere in CO2 incubator (EuroClone, Italy). Sulforhodamine B[33 (link)] was from Santa Cruz Biotechnology, Inc. Texas (USA). 3-(4,5-dimethylthiazol-2-yl)−2,5- diphenyltetrazolium bromide (MTT) was from (Sigma, USA).

Cells were plated into 96‐well plates (10³ cells/well) and left overnight at standard growth conditions. At each desired time point, cells were fixed with 50% Trichloroacetic acid at 4°C for 1 h. After removing fixative solution, cells were stained with 0.4% Sulforhodamine B (Santa Cruz) in 1% acetic acid. The dye taken up by cells was then dissolved in 10 mM Tris‐base, pH 10.5. The absorbance was measured at 560 nm with TriStar² Multimodal Reader LB942 (Berthold Technologies).

Human MCF7 breast cancer cells (ATCC® HTB-22), human PC3 prostate cancer cell line (ATCC® CRL-1435), A375 human skin cancer cell line (ATCC® CRL-1619), PANC1 pancreatic cell line (ATCC® CRL-1469), A549 lung cancer cell line (ATCC® CCL-185) and colorectal cancer cell line CACO2 (ATCC HTB-37) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% Foetal Bovine Serum (FBS) (Bio Whittaker, Verviers, Belgium), 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid HEPES Buffer (10 mM), gentamicin (50 mg/mL), L- glutamine (100 mg/mL), streptomycin (100 mg/mL), penicillin (100 mg/mL), (HEPES) Buffer, (Sigma, St. Luis, MO, USA) whereas Sulforhodamine B was from Santa Cruz Biotechnology, Inc. Texas, USA. Nicotinic acid derivatives (Sigma Aldrich, USA) were a gift of Professor Yusuf AL-Hiari (School of Pharmacy/University of Jordan), [Table 1], while the capsaicin was procured from Abcam (USA).