RNA from cells and tissues was extracted by TRIzol (Takara #9109) according to the manufacturer’s protocol. Then, reverse transcription (RT) was performed using the PrimeScriptTM RT reagent kit with gDNA eraser (Takara #RR047A) according to the protocol. Quantitative real-time polymerase chain reaction (RT-qPCR) was conducted using TB GreenTM Premix Ex TaqTM (Tli RNaseH Plus) (Takara #RR420A). The results were quantified using the 2−ΔΔCt method. In the present study, GAPDH and U6 were used as reference genes. The primers of miR-144-3p and U6 were purchased from RiboBio Co., Ltd., China. The primer sequences used are listed in Table S2 .
Primers of mir 144 3p and u6
Manufactured by RiboBio
Sourced in China
Primers of miR-144-3p and U6 are laboratory reagents used for the detection and quantification of specific ribonucleic acid (RNA) molecules. The miR-144-3p primer is designed to target the mature form of the microRNA miR-144-3p, while the U6 primer is used as a reference control. These primers are typically used in techniques such as reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to measure the expression levels of the target RNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using primers of mir 144 3p and u6
1
RNA Extraction, RT-qPCR for miRNA Expression
2
miR-144-3p Expression Analysis
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RNA from cells and tissues was extracted by TRIzol (Takara #9109) according to the manufacturer's protocol. Then, reverse transcription (RT) was performed using the PrimeScriptTM RT reagent kit with gDNA eraser (Takara #RR047A) according to the protocol. Quantitative real-time polymerase chain reaction (RT-qPCR) was conducted using TB GreenTM Premix Ex TaqTM (Tli RNaseH Plus) (Takara #RR420A). The results were quanti ed using the 2 -ΔΔCt method. In the present study, GAPDH and U6 were used as reference genes. The primers of miR-144-3p and U6 were purchased from RiboBio Co., Ltd., China. The primer sequences used are listed in table S2.
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