The specimens were stained with Mayer’s paracarmine (Merck, Darmstadt, Germany), dehydrated in an ethanol series, cleared in methyl salicylate and mounted in Canada balsam for morphological analysis. Specimens were then examined using a compound microscope equipped with a bright field (Leica DM 1000 LED microscope, Leica, Wetzlar, Germany). Measurements were taken using Leica Application Suite microscope software (Leica Microsystems GmbH, Wetzlar, Germany) and are given in micrometres as range followed by mean in parentheses. Some specimens from each locality were dehydrated in ethanol series, critical-point dried, sputter coated with gold, and examined with a Hitachi Stereoscan Model S-2469N scanning electron microscope operating at 15 kV. Adults and metacercariae were deposited in the Colección Nacional de Helmintos (CNHE) of the Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), México City (CNHE. No. 12063–12066).
Mayer s paracarmine
Manufactured by Merck Group
Sourced in Germany
Mayer's paracarmine is a laboratory staining reagent used in histology and cytology for the visualization of nuclei and other cellular structures. It is a form of carmine dye that is used to stain tissues and cells, providing contrast for microscopic examination. The core function of Mayer's paracarmine is to facilitate the identification and analysis of cellular components through staining.
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2 protocols using mayer s paracarmine
1
Morphological Analysis of Parasite Specimens
2
Staining and Microscopic Analysis of Digeneans
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Digeneans preserved in 100% ethanol were stained with Mayer's paracarmine (Merck, Darmstadt, Germany), dehydrated in ethanol series, cleared in methyl salicylate and mounted in Canada balsam for morphological analysis. Specimens were examined using a compound microscope equipped with a bright field Leica DM 1000 light emitting diode microscope (Leica, Wetzlar, Germany). Measurements were taken using Leica Application Suite microscope software (Leica Microsystems GmbH, Wetzlar, Germany) and are given in micrometres and presented with the range followed by the mean in parentheses. Some specimens were dehydrated with an ethanol series, critical point dried, sputter coated with gold and examined with a Hitachi Stereoscan Model S-2469N scanning electron microscope operating at 15 kV. Voucher specimens from the present study were deposited in the Colección Nacional de Helmintos (CNHE) from Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Mexico City.
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