The microbial growth, including the total viable count (TVC), coliforms (COS), and yeast and mold (YAM) of the shepherd’s purse, were determined by standard enumeration methods on a microbial media (PDA, Scharlau Chemie, SA., Barcelona, Spain).
The shepherd’s purse samples (5 ± 0.1 g) from the microbe group were homogenized with peptone Physiological Solution (PPS) (1 g/L peptone (Sinopharm Group Co., Ltd., Shanghai, China) + 8.5 g/L NaCl (Sinopharm)) in a sterile stomacher bag. Then, the decimal dilution series was prepared in PPS, and the appropriate dilutions were plated on different media for the enumeration of different microbial populations. The whole process was performed in a laminar flow bench (SJ-CJ-1D, Suzhou Purification Equipment Co. Ltd., Suzhou, China). The total viable count (TVC, Hopebio, Qingdao, China) was determined by spread plating on plate count agar (PCA, Hopebio) plates and incubated at 30 °C for 3 days. The cell counts of coliforms were determined on a MacConkey agar (Oxoid, Hampshire, UK) and incubated at 37 °C for 3 days. Yeasts and molds (Y&M, Hopebio) were counted on a Rose Bengal Chloramphenicol Agar (RBCA; Hopebio) and incubated at 22 °C for 5 days. All microbial counts were expressed as log CFU/g.
The shepherd’s purse samples (5 ± 0.1 g) from the microbe group were homogenized with peptone Physiological Solution (PPS) (1 g/L peptone (Sinopharm Group Co., Ltd., Shanghai, China) + 8.5 g/L NaCl (Sinopharm)) in a sterile stomacher bag. Then, the decimal dilution series was prepared in PPS, and the appropriate dilutions were plated on different media for the enumeration of different microbial populations. The whole process was performed in a laminar flow bench (SJ-CJ-1D, Suzhou Purification Equipment Co. Ltd., Suzhou, China). The total viable count (TVC, Hopebio, Qingdao, China) was determined by spread plating on plate count agar (PCA, Hopebio) plates and incubated at 30 °C for 3 days. The cell counts of coliforms were determined on a MacConkey agar (Oxoid, Hampshire, UK) and incubated at 37 °C for 3 days. Yeasts and molds (Y&M, Hopebio) were counted on a Rose Bengal Chloramphenicol Agar (RBCA; Hopebio) and incubated at 22 °C for 5 days. All microbial counts were expressed as log CFU/g.