Recombinant human light rhlight

Recombinant human LIGHT (rhLIGHT) was purchased from R&D Systems (Minneapolis, MN), and diluted in 0.1% BSA-PBS buffer. The CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega (Madison, WI, USA). StemPro^® MSC SFM CTS^™, StemPro^® Adipogenesis Differentiation Kit, StemPro^® Chondrogenesis Differentiation Kit, StemPro^® Osteogenesis Differentiation Kit, and fetal bovine serum (FBS) were obtained from GibcoBRL (Grand Island, NY, USA). Oil Red O staining kit (for adipocytes), Alcian blue staining kit (for chondrocytes) and Alizarin red staining (for osteocytes) were purchased from Invitrogen (Camarillo, CA, USA). The antibodies for western blotting, namely, anti-CDK2, cyclin E, and β-actin, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-p27, p-STAT3, p-Smad3, STAT3, Smad3, CDK1, cyclin B1, cyclin D1, and cyclin D3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-HVEM, LTβR, CD19, CD34, CD45, CD44, CD90, and CD105 antibodies and PI/RNase solution were purchased from BD Bioscience (San Jose, CA, USA). ELISA for PDGF-BB and TGF-β1 were purchased from R&D Systems. The Cell Proliferation ELISA, BrdU Assay Kit was purchased from Roche Diagnostics (San Francisco, CA, USA). All reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Recombinant human LIGHT (rhLIGHT) was purchased from R&D Systems (Minneapolis, MN, USA). Anti-human CD90-fluorescein isothiocyanate (CD90-FITC), anti-human CD44-FITC, anti-human CD105-FITC, anti-human CD34-FITC, anti-human CD45-FITC, anti-human CD19-FITC, anti-LTβR-PE, and FITC Mouse immunoglobulin G (IgG)-isotype control antibodies were purchased from BD Biosciences (San Diego, CA, USA). The Mesenchymal Stem Cell Growth Medium BulletKit™ (MSCGM™) was obtained from Lonza (Basel, Switzerland). For western blotting, antibodies against albumin, SRY-box transcription factor 17 (SOX17), forkhead box A2 (FOXA2), cyclin dependent kinase 1 (CDK1), CDK6, cyclin B1, cyclin D3, B-cell lymphoma-extra large (Bcl-xl), inhibitor of κB-α (IκB-α), protein kinase B (AKT), phospho-AKT, signal transducer and activator of transcription 3 (STAT3), phospho-STAT3, STAT5, phospho-STAT5, Rabbit IgG-horseradish peroxidase (IgG-HRP), and Mouse IgG-HRP were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against cytokeratin-18 (CK-18), CK-19, cytochrome P450 family 1 subfamily A member 1 (CYP1A1), CYP1A2, CYP3A4, CDK2, CDK4, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, Dallas, TX, USA). All reagents were obtained from Sigma–Aldrich (St. Louis, MO, USA).