3.0 t clinical mri scanner system

Firstly, the LTCA solution was placed in a quartz cuvette and irradiated by NIR laser for 10 min at 1 W/cm². C6 cells were seeded in 25 cm² flask and allowed to grow to ~80% density. Then, the medium was replaced by 2 mL of fresh medium containing various concentrations of LTCAs (I^-: 2, 4, 6, 8, and 10 μM). After incubation with LTCAs for 4 h, the medium was replaced by 1.5 mL of cell lysis reagent. The cell lysis solution was then collected for CT imaging. CT scans were performed using a GE Light Speed VCT imaging system at 80 kV and 100 mA, with a slice thickness of 0.625 mm. As a control group, the LTCA solution without irradiation was also evaluated as in the aforementioned procedure. In vitro light-triggered MRI imaging was determined by 3.0-T clinical MRI scanner system (Siemens, Berlin, Germany) at T1WI and T1 map sequences with TR 15 ms and TE 2.1 ms. The cell sample was prepared as in the aforementioned CT procedure; the incubated concentrations of Gd(III) were 0.06, 0.12, 0.18, 0.24, and 0.3 mM.

C6 tumor-bearing BALB/c nude mice were injected with the LTCA solution (200 μL) through the tail vein. After the injection, the tumor CT images of mice with or without laser irradiation were obtained with GE Light Speed VCT imaging system (80 KV, 100 mA, slice thickness 0.625 mm). The in vivo MRI imaging was also evaluated as in the aforementioned procedure and performed on a 3.0 T clinical MRI scanner system (Siemens) at T1WI, T1 map sequences with TR 15 ms and TE 2.1 ms. FI imaging was conducted with a PerkinElmer IVIS system (PerkinElmer, Inc., Santa Clara, USA). Tumor imaging before irradiation was used as control.