Materials rDIA gene was codon-optimized and synthesized by Sangon Inc. (Shanghai, China). Both of the E. coli BL21(DE3) and expression vector pET28a were purchased from Invitrogen Inc. (Carlsbad, USA). Protein marker, gene marker and restriction enzymes were obtained from Tiangen Inc. (Beijing, China). Ni-NTA columns, AKTA Pure chromatography system and PD-10 Desalting Columns were purchased from GE Healthcare. Coomassie brilliant blue G250, peptone, glycine and SDS-PAGE were purchased from Solarbio Inc (Beijing, China). Monopotassium, sodium hydrogen phosphate, ammonia chloride, sodium sulfate and magnesium sulfate were purchased from Tianjin jiangtian chemical technology Co. Ltd. (Tianjin, China). Unless noted, all used reagents were of analytical grade.
Both of the e coli bl21 de3 and expression vector pet28a
Manufactured by Thermo Fisher Scientific
Sourced in United States
E. coli BL21(DE3) is a bacterial strain commonly used for recombinant protein expression. It is a derivative of the E. coli B strain that contains the DE3 lysogen, which carries the T7 RNA polymerase gene under the control of the lac UV5 promoter. The pET28a vector is a plasmid commonly used for cloning and expression of recombinant proteins in E. coli. It features a T7 promoter, a polylinker region for inserting the gene of interest, and a kanamycin resistance marker for selection.
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Lab products found in correlation
Sds page, by Solarbio (2 mentions)
Akta pure chromatography system, by GE Healthcare (2 mentions)
Pd 10 desalting column, by GE Healthcare (2 mentions)
Ni nta column, by GE Healthcare (2 mentions)
Peptone, by Solarbio (2 mentions)
Coomassie brilliant blue g250, by Solarbio (2 mentions)
Glycine, by Solarbio (2 mentions)
Akta pure, by Cytiva (2 mentions)
2 protocols using both of the e coli bl21 de3 and expression vector pet28a
1
Recombinant Protein Expression in E. coli
2
Recombinant Protein Expression in E. coli
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Materials rDIA gene was codon-optimized and synthesized by Sangon Inc. (Shanghai, China). Both of the E. coli BL21(DE3) and expression vector pET28a were purchased from Invitrogen Inc. (Carlsbad, USA). Protein marker, gene marker and restriction enzymes were obtained from Tiangen Inc. (Beijing, China). Ni-NTA columns, AKTA Pure chromatography system and PD-10 Desalting Columns were purchased from GE Healthcare. Coomassie brilliant blue G250, peptone, glycine and SDS-PAGE were purchased from Solarbio Inc (Beijing, China). Monopotassium, sodium hydrogen phosphate, ammonia chloride, sodium sulfate and magnesium sulfate were purchased from Tianjin jiangtian chemical technology Co. Ltd. (Tianjin, China). Unless noted, all used reagents were of analytical grade.
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