Yeasts were grown on SD and after in Yeast Nitrogen Base broth (YNB -Sigma-Aldrich) at 37°C for 24 h each growth. Fungal suspension was centrifuged at 2000 rpm/10 min (MPW-350, Warsaw, Poland), supernatant was replaced with saline (0.9% NaCl) and another centrifugation was performed, this procedure was performed twice. Turbidity of the fungal suspension was adjusted in a spectrophotometer (530 nm; 0.381 ± 0.02) to obtain an inoculum of 10 7 CFU/mL. This standardized suspension was added in microplate wells (200 μL/well) and incubation (37°C/90 min) under agitation (75 rpm -Quimis, Diadema, Brazil) was performed. After preadherence of the fungal cells, supernatant was replaced with YNB broth and biofilm was formed for 48 h. Culture medium was exchanged after 24 h of incubation (de Oliveira et al. 2017) .
Yeast nitrogen base broth
Manufactured by Merck Group
Sourced in United States
Yeast Nitrogen Base broth is a microbiological culture medium used for the growth and maintenance of a variety of microorganisms, particularly yeast. It provides a balanced source of nitrogen, vitamins, and other essential nutrients required for microbial cultivation.
Automatically generated - may contain errors
Lab products found in correlation
Brain heart infusion broth, by HiMedia (1 mentions)
Menadione, by Merck Group (1 mentions)
P aeruginosa, by American Type Culture Collection (1 mentions)
P gingivalis, by American Type Culture Collection (1 mentions)
S mutans, by American Type Culture Collection (1 mentions)
C albicans, by American Type Culture Collection (1 mentions)
Hemin, by Merck Group (1 mentions)
S aureus, by American Type Culture Collection (1 mentions)
E faecalis, by American Type Culture Collection (1 mentions)
F nucleatum, by American Type Culture Collection (1 mentions)
P micra, by American Type Culture Collection (1 mentions)
Brucella broth, by Acumedia (1 mentions)
2 protocols using yeast nitrogen base broth
1
Fungal Biofilm Formation Protocol
2
Standardized Microbial Culture Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reference strains from American Type Culture Collection (ATCC) were used such as C. albicans (ATCC 18804), E. faecalis (ATCC 4083), P. aeruginosa (ATCC 15442), S. aureus (ATCC 6538), S. mutans (ATCC 35688), F. nucleatum (ATCC 25586), P. endodontalis (ATCC 35406), P. gingivalis (ATCC 33277), and P. micra (ATCC 23195). The microorganisms were frozen at -80 °C, and activated in a specific culture medium according to the metabolic needs, being Yeast Nitrogen Base broth (YNB -Sigma-Aldrich, St. Louis, USA), for C. albicans, and Brain Heart Infusion broth (BHI -Himedia), for aerobic bacteria. They were incubated at 37 °C for 24 h, with 5% CO2 for S. mutans. For anaerobic bacteria, Brucella broth (Acumedia, Michigan, USA) supplemented with 5% sterile defibrinated sheep blood (Newprov, Pinhais-PR, Brazil), 1% hemin (Sigma-Aldrich), and 1% menadione (Sigma-Aldrich) was used. These bacteria were incubated in anaerobiosis with nitrogen 80.01%, carbon dioxide 10.02%, and hydrogen 9.97%; (Whitley DG250 Workstation, West Yorkshire, UK) at 37 ºC for 48 h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!