Racemic tocol (50 mg mL -1 ) and tocopherols (α-, β-, γ-, and δ-isoforms) were purchased from Matreya (Pleasant Gap, PA, USA). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), potassium ferricyanide [K 3 Fe(CN) 6 ], ferric chloride (FeCl 3 ), trichloroacetic acid (TCA), β-carotene, linoleic acid, hydrochloric acid (HCl), sodium hydroxide (NaOH), penicillin/ streptomycin solution (100 U mL -1 and 100 mg mL -1 , respectively), α-glucosidase (isolated from Aspergillus Niger), and 4-p-nitrophenyl-α-D-glucopyranoside (4-pNPG) were purchased from Sigma-Aldrich. Mueller-Hinton agar, nutrient broth and Brain Heart Infusion broth (BHI) were purchased from Biolife (Monza, Italy). NaCl was obtained from CHEMI PHARMA, Tunisia. Hexane, chloroform and methanol were purchased from Merck (Darmstadt, Germany).
4 p nitrophenyl α d glucopyranoside 4 pnpg
Manufactured by Merck Group
4-p-nitrophenyl-α-D-glucopyranoside (4-pNPG) is a synthetic compound commonly used as a substrate in enzymatic assays. It is a glycoside composed of a p-nitrophenol group linked to an alpha-D-glucopyranoside moiety. The enzymatic cleavage of the glycosidic bond results in the release of p-nitrophenol, which can be detected spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Trichloroacetic acid, by Merck Group (2 mentions)
Hexane, by Merck Group (2 mentions)
Linoleic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Potassium ferricyanide, by Merck Group (2 mentions)
α glucosidase, by Merck Group (2 mentions)
β carotene, by Merck Group (2 mentions)
Mueller hinton agar (mha), by Biolife (1 mentions)
Racemic tocol, by Matreya (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
Nutrient broth, by Biolife (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl dpph 2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid diammonium salt, by Merck Group (1 mentions)
Brain heart infusion broth, by Biolife (1 mentions)
Butylated hydroxytoluene, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
2 protocols using 4 p nitrophenyl α d glucopyranoside 4 pnpg
1
Antioxidant and Enzyme Inhibition Assays
2
Antioxidant and Anti-Glucosidase Activities
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hexane, chloroform and methanol were purchased from Merck (Darmstadt, Germany). 1,1-diphenyl-2-picrylhydrazyl (DPPH), butylated hydroxytoluene (BHT), potassium ferricyanide [K 3 Fe(CN) 6 ], ferric chloride (FeCl 3 ), trichloroacetic acid (TCA), β-carotene, linoleic acid, hydrochloric acid (HCl), Sodium hydroxide (NaOH), α-Glucosidase (isolated from Aspergillus niger), 4-p-nitrophenyl-α-Dglucopyranoside (4-pNPG) were purchased from Sigma-Aldrich.
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