Lipofecatmine 2000 for cell transfection was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against MAZ, ZEB1 and ZEB2 were purchased from Abcam (Cambridge, MA, USA). Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit was from Cell Signaling technology (Danvers, MA, USA). Anti-β-actin antibody was from Signalway Antibody LLC (College Park, Maryland, USA). Unless otherwise noted, all other reagents were from Sigma-Aldrich (St. Louis, MO, USA).
Anti β actin antibody
Manufactured by Signalway Antibody
Sourced in United States
The Anti-β-actin antibody is a primary antibody that specifically binds to the beta-actin protein, a ubiquitous cytoskeletal protein found in all eukaryotic cells. This antibody can be used for the detection and quantification of beta-actin in various applications, such as Western blotting, immunocytochemistry, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Emt antibody sampler kit, by Cell Signaling Technology (2 mentions)
Epithelial mesenchymal transition antibody sampler kit, by Cell Signaling Technology (1 mentions)
Lipofecatmine 2000, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Goat anti mouse and anti rabbit secondary antibodies conjugated to horseradish peroxidase, by Cell Signaling Technology (1 mentions)
4 protocols using anti β actin antibody
1
Antibody-Based EMT Pathway Modulation
2
Antibody-Based EMT Pathway Analysis
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Antibodies against CBX8 (catalog No. ab182627), ZEB1 (catalog No. ab124512) and ZEB2 (catalog No. ab138222) were purchased from Abcam (Cambridge, MA, USA). Epithelial–mesenchymal transition (EMT) antibody sampler kit (catalog No. #9782) and phospho-β-catenin antibody (catalog No. #9561) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibody (catalog No. 21800) was from Signalway Antibody LLC (College Park, Maryland, USA). Lipofecatmine 2000 (catalog No. 11668019) for cell transfection and Trizol (catalog No. 15596018) for RNA extraction were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted.
3
Western Blot Analysis of CMTM2 and EMT
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After extracted from cells using RIPA buffer and quantified, the soluble protein was resolved by SDS-PAGE and transferred to PVDF membranes for Western blot using ECL detection reagents. CMTM2 antibody (catalog No. NBP1-59439) was obtained from Novus (Littleton, CO, USA), EMT antibody sampler kit (catalog No. #9782) from Cell Signaling Technology (Danvers, MA, USA) and anti-β-actin antibody (catalog No. 21800) from Signalway Antibody LLC (College Park, Maryland, USA).
4
Western Blot Analysis of MIRA-1-Treated MM Cells
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MIRA-1-treated MM cells were harvested to isolate proteins. Whole cell lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to PVDF membrane (Millipore, Billerica, MA, USA), as described previously (Saha et al, 2010b (link), 2010c (link), 2010d (link); Saha et al, 2012 (link); Saha et al, 2013b (link)). Immunoblotting was performed using specific antibodies: anti-p53 (DO-7) (Sigma-Aldrich); Mcl-1, caspase-3, and PARP (Cell Signaling Technologies, Beverly, MA, USA); Puma, Bax, and c-Myc (Signalway Antibody, College Park, MD, USA); XBP1 and p-PERK (Thr 981) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Goat anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase were purchased from Cell Signaling and Santa Cruz Biotechnology, respectively. Blots were reprobed with anti-β actin antibody (Signalway Antibody) for equal protein controls.
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