The human DLBCL-derived cell lines SU-DHL-4, SU-DHL-6, OCI-LY19, U-2932, and Ri-1 were obtained from the German Collection of Microorganism and Cell Cultures, Department of Human and Animal Cell Cultures; DB, SU-DHL-8, and the BL cell lines RAJI, Ramos, and Daudi were obtained from ATCC. The DLBCL-derived cell lines (HBL-1 and TMD8) were provided by R. E. Davis, MD Anderson Cancer Center, Houston. Cell lines were cultured in RPMI medium 1640 supplemented with 10 or 20% heat-inactivated FBS (Gibco BRL), 1% l -glutamine, and penicillin–streptomycin in a humid environment of 5% CO2 at 37 °C.
Su dhl 8
Manufactured by American Type Culture Collection
Sourced in United States
The SU-DHL-8 is a laboratory equipment product designed for cellular and microbiological applications. It serves as a high-capacity, temperature-controlled incubator for cell culture and microbial growth. The device offers precise temperature regulation and programmable settings to support various experimental requirements.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (5 mentions)
Su dhl 4, by American Type Culture Collection (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Oci ly10, by American Type Culture Collection (2 mentions)
Oci ly1, by American Type Culture Collection (2 mentions)
Su dhl 10, by American Type Culture Collection (2 mentions)
Jeko 1, by American Type Culture Collection (2 mentions)
Heat inactivated fbs, by Thermo Fisher Scientific (1 mentions)
Oci ly01, by American Type Culture Collection (1 mentions)
Facscalibur instrument, by BD (1 mentions)
Nudul 1, by American Type Culture Collection (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Pyrrolidine dithiocarbamate, by Merck Group (1 mentions)
Su dhl 2, by American Type Culture Collection (1 mentions)
Oci ly3, by Leibniz Institute DSMZ (1 mentions)
Rec 1, by American Type Culture Collection (1 mentions)
Jvm 2, by American Type Culture Collection (1 mentions)
Rgfp966, by Selleck Chemicals (1 mentions)
13 protocols using su dhl 8
1
Cell Line Culture Protocol for DLBCL
2
Lentiviral Knockdown of CD300A in DLBCL Cell Lines
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Human DLBCL cell lines OCI-Ly01, VAL, OCI-Ly10, SUDHL-8, Farage, and SUDHL-4 cells were purchased from ATCC (Shanghai, China) and cultured in RPMI-1640 medium (Hyclone, Utah, USA) supplemented with 10% fetal bovine serum (FBS) (Ausgenex, Australia), 100 U/mL penicillin, 100 U/mL streptomycin, and 2 mM L-glutamine. Human embryonic kidney 293T cells were maintained in Dulbecco modified Eagle media (DMEM; Hyclone) with 10% FBS. Cells were cultured at 37°C in a 5% CO2 humidified incubator.
Lentiviral plasmids containing optimized CD300A short hairpin (shRNA) or scramble sequences were produced using 293T cells, and were used to infect DLBCL cells as described [4 (link), 33 (link)]. The sequences for CD300A shRNAs used in the present study included shRNA-1 (5′-GATGTTTCAGAAATGGATCAA-3′), shRNA-2 (5′-CCCAGGGAAGAACTTCACTAT-3′), and a scramble shRNA (5′-CCTAAGGTTAAGTCGCCCTCG-3′).
The surface expression of CD300A in lymphoma cells was determined using a phycoerythrin (PE)-conjugated antibody against human CD300A (clone E59.126, Beckman Coulter, Marseille, France). After staining for 30 min on ice, cells were resuspended in phosphate-buffered saline (PBS) and analyzed using a FACS Calibur instrument (Becton Dickinson, CA, USA). The isotypic antibody (clone 679.1Mc7, Beckman Coulter) was used as control.
Lentiviral plasmids containing optimized CD300A short hairpin (shRNA) or scramble sequences were produced using 293T cells, and were used to infect DLBCL cells as described [4 (link), 33 (link)]. The sequences for CD300A shRNAs used in the present study included shRNA-1 (5′-GATGTTTCAGAAATGGATCAA-3′), shRNA-2 (5′-CCCAGGGAAGAACTTCACTAT-3′), and a scramble shRNA (5′-CCTAAGGTTAAGTCGCCCTCG-3′).
The surface expression of CD300A in lymphoma cells was determined using a phycoerythrin (PE)-conjugated antibody against human CD300A (clone E59.126, Beckman Coulter, Marseille, France). After staining for 30 min on ice, cells were resuspended in phosphate-buffered saline (PBS) and analyzed using a FACS Calibur instrument (Becton Dickinson, CA, USA). The isotypic antibody (clone 679.1Mc7, Beckman Coulter) was used as control.
3
Cell Line Maintenance Protocol
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SU-DHL-4 (CRL-2957), NU-DUL-1 (CRL-2969), and SU-DHL-8 (CRL-2961) cell lines were purchased from the Non-Hodgkin’s Lymphoma Cell Line Panel at ATCC (Manassas, VA, USA) and maintained in RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific). All cell lines were cultured at 37°C under 5% CO2. Media was refreshed every 48 h.
4
Cell Culture Protocols for DLBCL and T Cell Lines
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DLBCL cells SU‐DHL‐8 and OCI‐LY1 were purchased from the ATCC and Shanghai Jingkang Bioengineering Co., Ltd., respectively. A human lymphoblastoid cell line AHH‐1 was acquired from Mingzhoubio. CD8+ T cells were purchased from BlueFBio.
SU‐DHL‐8, OCI‐LY1, and AHH‐1 cells were cultured in RPMI‐1640 medium plus 10% fetal bovine serum (FBS), 2 mmol/Ll ‐glutamine and 100 U/mL penicillin/streptomycin at 37°C in a 5% CO2 incubator. CD8+ T cells were grown in Dulbecco's modified Eagle's medium plus 10% FBS and 100 units/mL penicillin/streptomycin at 37°C in a 5% CO2 incubator. All reagents for cell culture were acquired from Thermo Fisher Scientific Inc.
SU‐DHL‐8, OCI‐LY1, and AHH‐1 cells were cultured in RPMI‐1640 medium plus 10% fetal bovine serum (FBS), 2 mmol/L
5
Molecular Profiling of DLBCL Tissue
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Forty-five cases of lymphoma tissues were obtained from DLBCL patients who were diagnosed and had undergone surgical resection at Xianning Central Hospital (Hubei, China) between August 2010 to March 2016. Additionally, 23 cases of reactive lymphoid hyperplasia tissues were collected in the same period, and were selected as negative controls. No local or systemic therapy was conducted in these participators before operation. Primary CD19+ cells were obtained from the serum of healthy volunteers through positive selection with CD19+ MicroBeads antibody (Miltenyi Biotec, Auburn, CA, USA). Written informed consent was obtained from all participants, and the study was approved by Institutional Review Board of Xianning Central Hospital.
Three DLBCL cell lines (SU-DHL-8, OCI-LY1, and SU-DHL-10) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), and were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Tempe, AZ, USA) plus 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 2 mmol/L L-glutamine (Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin (Thermo Fisher Scientific), at 37℃ in 5% CO2.
Three DLBCL cell lines (SU-DHL-8, OCI-LY1, and SU-DHL-10) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), and were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Tempe, AZ, USA) plus 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 2 mmol/L L-glutamine (Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin (Thermo Fisher Scientific), at 37℃ in 5% CO2.
6
B-Lymphoma Cell Line Culturing
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Human B-lymphoma cell lines SU-DHL-4, SU-DHL-8, human umbilical vein endothelial cell (HUVEC), and murine B-lymphoma cell line A20 were obtained from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in humidified atmosphere of 95% air and 5% CO2 at 37 °C. ABT-199 was purchased from Selleck-Biotool (Houston, TX, USA). Anti-Human ICOS functional grade purified antibody was from Affymetrics Ebioscience (San Diego, CA, USA).
7
DLBCL Cell Line Cultivation
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Male NOD/SCID mice aged 6–8 weeks were purchased from the Laboratory Animal Center, the Academy of Military Medical Sciences, (Peking, China). All animal experiments were performed according to Health guidelines of Nankai University Institutional Animal Use and Care Committee. The normal B cell line HMy2. CIR was purchased from the institutes for biological sciences of Chinese academy of sciences (Shanghai, China). DLBCL lines SU-DHL-2 and SU-DHL-8 were purchased from American Type Culture Collection (Manassas, VA). Five DLBCL cell lines (OCI-Ly3, OCI-Ly19, SU-DHL-4, SU-DHL-6 and VAL) were the kind gift of Dr. Jun Cheng (Tianjin Medical University Cancer Institute and Hospital, Tianjin, China). Pyrrolidine dithiocarbamate (PDTC) was purchased from Sigma-Aldrich (#P8765, St Louis, MO).
8
Cell Line Characterization and Culture
9
DLBCL Cell Line Maintenance Protocol
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The DLBCL cell line (SU-DHL-8) was purchased from American Type Culture Collection (Bethesda, MD, USA). Cells were maintained in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine in a humidified incubator with 5% CO2 at 37 °C.
10
Cell Culture Conditions for Lymphoma Models
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BLBCL cell lines (SU-DHL-8 and OCI-LY-8) and normal lymphoblast cell line (C1R-neo) were used in this study. C1R-neo and SU-DHL-8 cells were obtained from American Type Culture Collection (Manassas, VA, USA) and OCI-LY-8 cells were bought from Shanghai Zishi Biological Technology Co., Ltd. (Shanghai, China). Streptomycin/penicillin (100 U/mL; Invitrogen, Carlsbad, CA, USA) and 10% fetal bovine serum (FBS; Invitrogen) were added into Roswell Park Memorial Institute 1640 (RPMI-1640) medium (Gibco, Carlsbad, CA, USA), which was used to culture cells at a humidified chamber at 37°C supplemented with 5% CO2.
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