A549 cis cells

The CIS-resistant NSCLC cells (A549/CIS cells) and Human normal lung epithelial cells (BEAS-2B cells) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured (37°C, 5% CO₂) in DMEM medium (10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin).

Soya lecithin (SL, injectable) was provided by Lipoid GmbH (Ludwigshafen, Germany). Compritol^® 888 ATO (ATO) was a gift from Gattefossé (Saint-Priest, Lyon, France). DSPE-PEG₂₀₀₀-COOH was produced by Seebio Biotech (Shanghai) Co., Ltd. (Shanghai, China). Transferrin, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-Hydroxysuccinimide (NHS), and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were bought from Sigma Aldrich (St. Louis, MO). The CIS resistant NSCLC cell line (A549/CIS cells), another NSCLC cell line (H1975 cells), and human normal lung epithelial cells (BEAS-2B cells) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in RPMI Medium 1640 supplemented with 10% (v/v) FBS and 1% (v/v) penicillin–streptomycin at 37°C in an atmosphere of 5% CO₂.