For the preparation of nanoparticles: Magnetic stirrer (C-MAG HS 7, IKA -Staufen, Germany), pHmeter (D-22, Digimed -São Paulo, Brazil). Determination of Particle size, polydispersity index, and zeta potential: Malvern Zetasizer Nano S (Malvern Instruments, Worcestershire, UK). Enzymatic activity: UV/Vis Spectrophotometer (Libra S22, Biochrom -Cambridge, UK), centrifuge (Rotina 380 R Benchtop, Andreas Hettich GmbH & Co. KG -Tuttlingen, Germany). For Fourier transform infrared spectroscopy (FTIR), Thermogravimetric and Differential scanning calorimetry (DSC) analyzes were used: Cary 630 Spectrophotometer (Agilent Technologies -California, USA), SDT equipment model Q600 (TA Instruments -Delaware, USA), DSC-50 cell (Shimadzu -Kyoto, Japan), respectively. For Scanning electron microscopy (SEM) was used an Inspect F50 microscope (FEI Company -Tokyo, Japan).
Rotina 380 r benchtop
Manufactured by Hettich
Sourced in United Kingdom, Germany
The Rotina 380 R Benchtop is a centrifuge designed for general laboratory use. It features a capacity of up to 4 x 100 mL and a maximum speed of 4,000 rpm. The centrifuge includes a brushless motor and an imbalance detection system.
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Lab products found in correlation
2 protocols using rotina 380 r benchtop
1
Nanoparticle Characterization Protocol
2
Nanoparticle Encapsulation Efficiency Determination
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Nanoparticles containing I3C were centrifuged at 10,000 xg, 4 • C for 1 h in a Rotina 380 R Benchtop centrifuge (Andreas Hettich GmbH & Co. KG -Tuttlingen, Germany). EE, defined as the amount of drug associated with the nanoparticles, was determined (Eq. ( 1)):
where I3C o is the concentration of total drug offered during the encapsulation process and I3C f represents the free drug present in the dispersion supernatant, determined after centrifugation. The quantification was performed on a spectrophotometer (280 nm), and the I3C concentration was estimated through the previously established standard curve.
where I3C o is the concentration of total drug offered during the encapsulation process and I3C f represents the free drug present in the dispersion supernatant, determined after centrifugation. The quantification was performed on a spectrophotometer (280 nm), and the I3C concentration was estimated through the previously established standard curve.
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