Catalog no 11360 070

The SaOS2-LM7 and its parent cell line SaOS2 were a kind gift from Dr. Eugenie Kleinerman, The University of Texas M.D. Anderson Cancer Center, USA. HOS (Catalog no. CRL-1543) and HOS-143B (Catalog no. CRL-8303) were purchased from ATCC. All cell lines were cultured in Minimal Essential Medium (MEM) (Catalog no. 10320-021, Gibco), supplemented with 10% fetal bovine serum (FBS) (Catalog no. 12483-020, Gibco), 1% penicillin-streptomycin (Catalog no. 15140-122, Gibco), 1mM sodium pyruvate (Catalog no. 11360-070, Gibco) and 2mM L-Glutamine (Catalog no. 25030-081, Gibco) at 37°C and 95% O2 and 5% CO2.

OG2 ESCs were isolated in a previous study [52] (link). For the derivation of OG2 EpiSCs, the late epiblast layer was dissected intact from a pre-gastrulation stage mouse blastocyst (E5.75), maintained in FA medium [DMEM/F12 (Catalog No. SH30023.01B, Hyclone, Buckinghamshire, UK) and Neurobasal medium (Catalog No. 21103049, Gibco, Carlsbad, CA) mixed 1:1, supplemented with 1× non-essential amino acids (Catalog No. 11140050, Gibco), 1× GlutaMAX (Catalog No. 35050061, Gibco), 1 mM sodium pyruvate (Catalog No. 11360070, Gibco), 50 U/ml penicillin/streptomycin (Catalog No. SV30010, Hyclone), 1× N-2 (Catalog No. 17502048, Gibco), 1× B-27 (Catalog No. 17504044, Gibco), 12.5 ng/ml bFGF (Catalog No. 233-FB-050, R & D, Minneapolis, MN), and 20 ng/ml Activin A (Catalog No. 338-AC-050, R & D)] on Matrigel (Catalog No. 354248, Corning, Corning, NY)-coated plates and passaged 4–8 days after isolation.