Proteins from cell cultures were extracted in lysis buffer (radioimmunoprecipitation assay; Cell Signaling), and protein concentrations were assessed using the Bradford Protein Assay Kit (Thermo Fisher). Protein samples were separated on a 4–12% SDS-PAGE gel and transferred onto nitrocellulose membranes using semi-dry transfer (Bio-Rad). To prevent nonspecific binding, membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 for 1 h at room temperature. The following antibodies were used: anti-TAP1 (dilution 1:1,000, 4°C; Cell Signaling), anti–β-actin (dilution 1:1,000, 4°C; Cell Signaling), and HRP-linked mouse anti-rabbit IgG (dilution 1:5,000, room temperature; Cell Signaling).
Anti tap1
Manufactured by Cell Signaling Technology
Anti-TAP1 is a laboratory reagent used to detect the presence and quantify the levels of the TAP1 protein in biological samples. TAP1 is a component of the transporter associated with antigen processing (TAP) complex, which plays a critical role in the immune response by transporting peptides into the endoplasmic reticulum for presentation on MHC class I molecules. The Anti-TAP1 reagent can be used in various immunodetection techniques, such as Western blotting, immunoprecipitation, and flow cytometry, to study the expression and function of the TAP1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Semi dry transfer, by Bio-Rad (1 mentions)
Bradford protein assay kit, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti cyclophilin b, by Abcam (1 mentions)
Anti ovalbumin, by Santa Cruz Biotechnology (1 mentions)
Anti bptf, by Merck Group (1 mentions)
Anti psmb8, by Cell Signaling Technology (1 mentions)
Anti mouse or anti rabbit horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Tri reagent, by Merck Group (1 mentions)
Anti histone h3, by Abcam (1 mentions)
Anti β actin ac 15, by Merck Group (1 mentions)
3 protocols using anti tap1
1
Western Blot Protein Analysis
2
Immunoblotting of Cell Signaling Proteins
3
Antibody Panel for Western Blotting
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The following primary antibodies were used for western blotting (WB): anti-SMYD3 (D2Q4V) (rabbit #12859); Cell Signaling Technology; dilution used in WB: 1:1000, anti-TAP1 (rabbit #12341); Cell Signaling Technology; dilution used in WB: 1:1000, anti-histone H3 (tri methyl K4) (ab8580); Abcam; dilution used in WB: 1:10000, anti-histone H3 (ab1791); Abcam; dilution used in WB: 1:20000), anti-β-actin (AC-15) (mouse #A5441); Sigma-Aldrich; dilution used in WB: 1:5000.
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