Ab117819

Manufactured by Abcam

Ab117819 is a recombinant monoclonal antibody. It is a laboratory product intended for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Wortmannin, by Thermo Fisher Scientific (1 mentions) Ab105459, by Abcam (1 mentions) Rapamycin, by Merck Group (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Lysotracker deep red, by Thermo Fisher Scientific (1 mentions) Mouse anti lamp1, by BD (1 mentions) Bafilomycin, by Merck Group (1 mentions) Mouse anti gfp, by Roche (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) Mouse anti actin, by Proteintech (1 mentions) Rabbit anti fip200, by Proteintech (1 mentions) Rabbit anti fip200, by Merck Group (1 mentions) Rabbit anti vapa, by Proteintech (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Rabbit anti atg13, by Cell Signaling Technology (1 mentions) Sab4200135, by Merck Group (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Rabbit anti lc3, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Ma5 15738, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab117819

Autophagy Protein Immunodetection Assays

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The following antibodies were used for immunostaining or immunoblotting assays: mouse anti-LC3 (MBL, M152-3), rabbit anti-LC3 (2775S; Cell Signaling Technology), rabbit anti-p62 (MBL, PM045), rabbit anti-FIP200 (SAB4200135; Sigma-Aldrich), rabbit anti-FIP200 (17250-1-AP; Proteintech), rabbit anti-ULK1 (8054; Cell Signaling Technology), rabbit anti-Atg13 (13468S; Cell Signaling Technology), rabbit anti-ATL2 (16688-1-AP; Proteintech), rabbit anti-ATL3 (ab117819; Abcam), mouse anti-WIPI2 (ab105459; Abcam), mouse anti-VAPB (66191-1-IG; Proteintech), rabbit anti-VAPA (15275-1-AP; Proteintech), mouse anti-LAMP1 (553792; BD Biosciences), rabbit anti-PTPIP51 (20641-1-AP; Proteintech), rabbit anti-ATG101 (13492; Cell Signaling Technology), rabbit anti-Myc (2278S; Cell Signaling Technology), mouse anti-Myc (M5546; Sigma-Aldrich), mouse anti-GFP (11814460001; Roche), mouse anti-actin (60008-1-IG; Proteintech), and rabbit anti-HA (H6908; Sigma-Aldrich).
The following reagents were used in this study: wortmannin (PHZ1301; Life Technologies), bafilomycin (B1793; Sigma-Aldrich), rapamycin (R0395; Sigma-Aldrich), Lipofectamine 2000 (12566014; Life Technologies), Lipofectamine RNAi MAX (13778150; Life Technologies), and LysoTracker Deep Red (L12492; Thermo Fisher Scientific).

Liu N., Zhao H., Zhao Y.G., Hu J, & Zhang H. (2021). Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. The Journal of Cell Biology, 220(7), e202012091.

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ATL2 Antibody Production and Characterization

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Anti-ATL2 rabbit polyclonal antibodies were custom-raised against the his₆-tagged cytosolic domain (1−479 a.a.) of ATL2 (AbFrontier). These antibodies were affinity-purified using their antigens bound to SulfoLink resin (20401; Thermo Fisher Scientific). Anti-ATL3 rabbit polyclonal (ab117819; Abcam), anti-myc mouse monoclonal (2276S; Cell Signaling technology), anti-GFP mouse monoclonal (MA5-15256; Invitrogen), anti-Gluc rabbit polyclonal (E8023; New England BioLabs), anti-calnexin mouse monoclonal (sc-23954; Santa Cruz), anti-GAPDH mouse monoclonal (MA5-15738; Thermo Fisher Scientific), and anti-protein disulfide isomerase mouse monoclonal (MA3-019; Invitrogen) antibodies were purchased. Anti-Yet3p rabbit sera were a generous gift from C. Barlowe (Geisel School of Medicine at Dartmouth). Alexa Fluor 594-conjugated anti-mouse goat IgG (A-11005) was purchased from Invitrogen. Peroxidase-conjugated anti-mouse goat IgG (AB_10015289) and peroxidase-conjugated anti-rabbit goat IgG (AB_2313567) were purchased from Jackson ImmunoResearch.

Jang E., Moon Y., Yoon S.Y., Diaz J.A., Lee M., Ko N., Park J., Eom S.H., Lee C, & Jun Y. (2023). Human atlastins are sufficient to drive the fusion of liposomes with a physiological lipid composition. The Journal of Cell Biology, 222(4), e202109090.

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