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Monoclonal anti myc and anti his alkaline phosphatase conjugated antibodies

Manufactured by Merck Group

Monoclonal anti-Myc and anti-His alkaline phosphatase (AP) conjugated antibodies are laboratory reagents used for detection and purification of proteins that contain Myc or His tags. These antibodies are conjugated to the enzyme alkaline phosphatase, which can be used to generate a colorimetric or chemiluminescent signal when the antibody binds to the target protein.

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2 protocols using monoclonal anti myc and anti his alkaline phosphatase conjugated antibodies

1

Isolation and Modification of LDL

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Plasma was obtained from healthy donors after an overnight fast following consent under a protocol approved by the UCSD Human Research Protections Program. LDL was isolated by sequential ultracentrifugation, and modified with malondialdehyde (MDA), malondialdehyde-acetaldehyde-adducts (MAA) or CuSO4 to generate MDA-LDL, MAA-LDL or copper-oxidized LDL (Cu-OxLDL) respectively, as previously described31 (link). Phosphocholine-BSA (PC-BSA) was from Biosearch Technologies and POVPC-BSA was prepared as described32 (link). Monoclonal anti-Myc and anti-His alkaline phosphatase (AP) conjugated antibodies were from Sigma.
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2

Isolation and Modification of LDL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma was obtained from healthy donors after an overnight fast following consent under a protocol approved by the UCSD Human Research Protections Program. LDL was isolated by sequential ultracentrifugation, and modified with malondialdehyde (MDA), malondialdehyde-acetaldehyde-adducts (MAA) or CuSO4 to generate MDA-LDL, MAA-LDL or copper-oxidized LDL (Cu-OxLDL) respectively, as previously described31 (link). Phosphocholine-BSA (PC-BSA) was from Biosearch Technologies and POVPC-BSA was prepared as described32 (link). Monoclonal anti-Myc and anti-His alkaline phosphatase (AP) conjugated antibodies were from Sigma.
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