In the OFT, locomotor activity as total traveled distance, average speed of each animal, time spent on the periphery, and time spent in the center were measured. We also observed exploratory behaviors in terms of rearing, defecating, and comfort behaviors when washing. At the start of the test, the rats were placed in the center of the apparatus. Each trial lasted 6 min. The progress of the animals in OFT was recorded and evaluated using the computerized video-tracking system Smart Junior (Panlab, Barcelona, Spain).
Smart junior
Manufactured by Harvard Apparatus
Sourced in Spain
The Smart Junior is a compact, precise, and programmable syringe pump designed for a wide range of laboratory applications. It features a user-friendly interface, multiple program options, and accurate flow rate control.
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Lab products found in correlation
6 protocols using smart junior
1
Measuring Rodent Locomotor Activity
2
Spatial Memory Assessment using Morris Water Maze
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The Morris water maze task was performed according to the previous method [22 (link)]. Briefly, a circular pool (100 cm diameter 3 × 50 cm height) with a circular acrylic platform (10 cm diameter 3 × 35 cm height) was used and location of platform can be discriminated by visual cue. The pool was filled with milk water (22 ± 1°C) and divided into equal quadrants [23 (link)]. Data were recorded using a video camera connected to the corresponding software (Smart Junior, Panlab SL; Barcelona, Spain). Mice were placed on the platform for 10 s and removed from the pool. Mice were given acquisition trial for 4 days. The escape latency and cumulative path-length were recorded during each acquisition trial. On day 5th, all mice were subjected to probe trial without platform and were recorded for 120 s. The time spent in the target quadrant was measured for spatial learning and memory.
3
Comprehensive Evaluation of Fatigue and ME/CFS Symptoms
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A total of eight behavioral tests assessing fatigue-like behaviors (rota-rod test, fatigue rotating wheel test and home-cage activity test) and ME/CFS symptoms (nest building test for general malaise, plantar test for pain sensitivity, grip strength test for muscular strength, open field test for anxiety, and novel object recognition test for memory impairment) were conducted (Additional file 1 : Fig. S1A, Exp. 1 and 2). All behaviors were recorded, and tracking data were calculated using a video camera connected to corresponding software (Smart Junior, Panlab SL, Barcelona, Spain). Performance was evaluated by researchers blinded to the experimental conditions. The detailed methods of the eight behavioral tests are described in the Additional file 1 .
4
Open-Field Test for Locomotor Activity
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The open‐field test was performed between 9:00 am and 1:00 pm as previously reported (Haurigot et al, 2013 ). Briefly, animals were placed in the center of a brightly lit chamber (41 × 41 × 30 cm) crossed by two bundles of photobeams (LE 8811; Panlab) that detect horizontal and vertical movements. Motor and exploratory activities were evaluated during the first 6 min. The total distance covered was evaluated using a video tracking system (SMART Junior; Panlab).
5
Open-Field Locomotor Activity Assessment
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The apparatus consists of a circular arena (1 m of diameter) limited by a 40 cm high wall and illuminated by four 60 W lamps situated 48 cm above the arena floor, providing illumination around 300 lx. The animals were gently placed in the center of the arena and were allowed to freely explore the area for 5 min. During the experiments, the open-field was video recorded, and the measures of total distance were computed online by an image analyzer system (Smart junior, PanLab, Harvard Apparatus, Spain).
6
Open Field Exploration Behavior
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The apparatus consists of a circular arena ( 1 m of diameter) limited by a 40 cm high wall and illuminated by four 60 W lamps situated 48 cm above the arena floor, providing illumination around 300 lx26 . The animals were gently placed in the center of the arena and were allowed to freely explore the area for 5 min. During the experiments, the open field test was video recorded and the measures of locomotion of the groups were computed by an image analyzer system (Smart junior, PanLab, Harvard Apparatus, Spain).
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