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Mab628

Manufactured by R&D Systems

MAB628 is a monoclonal antibody that specifically recognizes and binds to a specified target antigen. This product is intended for laboratory research use only.

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2 protocols using mab628

1

In Vitro Stimulant Experiments

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The following stimulants were used for in vitro experiments (concentration and vendor in parentheses): MDP (0.5 or 1 μg ml−1, Invivogen; 53678–77-6), lipid A (0.5 μg ml−1, innaxon IAX100–004-C250), Pam3Cys (2.5 μg ml−1, Sigma-Aldrich; 506350–2MG), BZA (1 or 2 μM, Selleck Chemicals; S2128); and human anti-gp130 monoclonal antibody (0.05 μg ml−1, R&D Systems; MAB628).
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2

Fibrosis Signaling Pathway Analysis

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BIP (3177, CST), Cleaved-Caspase 3 (9664, CST), Caspase 3 (9662, CST), CHOP (5554, CST), Collagen I (ab34710, Abcam), phospho-ERK1/2 (4370, CST), ERK1/2 (4695, CST), GAPDH (2118, CST), neutralizing anti-human gp130 (MAB628, R&D Systems), anti-gp130 (PA5-99526, Thermo Fisher), gp130 (PA5-28932, Thermo Fisher, IF), IgG (11E10, Aldevron), neutralizing anti-IL11 (X203, Aldevron), commercial anti-IL11 (MAB218, R&D Systems), neutralizing anti-IL11RA (X209, Aldevron), IL11RA (ab125015, abcam, IF), phospho-mTOR (2971, CST), mTOR (2972,CST), phospho-p70S6K (9205, CST), p70S6K (2708, CST), p-PDGFRB (3124, CST), PDGFRB (3169, CST), phospho-S6 ribosomal protein (4858, CST), S6 ribosomal protein (2217, CST), ⍺-SMA (ab7817, Abcam, Operetta), ⍺-SMA (19245, CST, WB), phospho-SMAD2 (3108, CST), SMAD2 (5339, CST), phospho-STAT3 (4113, CST), STAT3 (4904, CST), XBP-1 (sc-8015, SantaCruz), mouse Alexa Fluor 488 secondary antibody (ab150113, Abcam), mouse HRP (7076, CST), rabbit Alexa Fluor 488 secondary antibody (ab150077, Abcam), rabbit HRP (7074, CST).
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