Tomato lectin fluorescein

Manufactured by Vector Laboratories

Tomato lectin-fluorescein is a laboratory reagent produced by Vector Laboratories. It is a fluorescently labeled lectin, specifically derived from the tomato plant. Lectins are proteins that bind to carbohydrates, and the fluorescein label allows for visualization and detection of the lectin-carbohydrate interactions.

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Lab products found in correlation

Donkey anti goat cy5, by Jackson ImmunoResearch (2 mentions) Cy2 donkey anti rabbit, by Jackson ImmunoResearch (2 mentions) Donkey anti rabbit cy3, by Jackson ImmunoResearch (2 mentions) Donkey anti mouse cy3, by Jackson ImmunoResearch (2 mentions) Anti sma, by Agilent Technologies (1 mentions) M0851, by Agilent Technologies (1 mentions)

Spelling variants of this product

Tomato lectin (21 citations) Lectins (7 citations) Fluorescein lycopersicon esculentum (tomato) lectin (5 citations) Tetramethylrhodamine β-isothiocyanate (TRITC)- or fluorescein isothiocyanate (FITC)-conjugated Lycopersicon esculentum agglutinin (LEA) tomato lectin (2 citations) Fluorescein-conjugated tomato lectin (2 citations)

2 protocols using tomato lectin fluorescein

Multicolor Immunofluorescence of Vasopressin

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The following primary and secondary antibodies were used: anti-AVP (rabbit, ImmunoStar, 20069, Hudson, WI); antitype IV collagen (goat, SouthernBiotech, 1340-01, Birmingham, AL); anti-SMA (1:67, mouse, Dako, M0851, Santa Clara, CA): donkey antirabbit Cy2 (Jackson ImmunoResearch, 711-225-152, West Grove, PA); donkey antirabbit Cy3 (Jackson ImmunoResearch, 711-165-152); donkey antimouse Cy3 (Jackson ImmunoResearch, 715-165-151); donkey antigoat Cy5 (Jackson ImmunoResearch, 705-175-147); tomato lectin-fluorescein (Vector laboratories, FL-1171, Burlingame, CA).

Yao Y., Taub A.B., LeSauter J, & Silver R. (2021). Identification of the suprachiasmatic nucleus venous portal system in the mammalian brain. Nature Communications, 12, 5643.

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Multimarker Labeling of Vasculature and SCN

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The following primary antibodies were used in the iDISCO protocol: for labeling the SCN, AVP antibody (1:2500, rabbit; ImmunoStar, Hudson, WI); for labeling arteries, smooth muscle actin (SMA) antibody (1:67, mouse; Dako, M0851, Santa Clara, CA); for labeling the entire vasculature, Type IV Collagen-UNLB antibody (1:50, goat; SouthernBiotech, Birmingham, AL). For the sections, the rabbit AVP antibody was used at 1:5000. The following secondary antibodies were used in the iDISCO protocol; for AVP, donkey anti-rabbit Cy2 (1:200; Jackson ImmunoResearch); for SMA, donkey anti-mouse Cy3 (1:200; Jackson ImmunoResearch, West Grove, PA); for collagen, donkey anti-goat Cy5 (1:200; Jackson ImmunoResearch), and for sections, for AVP, donkey anti-rabbit Cy3 (1:200; Jackson ImmunoResearch); for the entire vasculature, tomato lectin-fluorescein (1:100; Vector Labs, Newark, CA).

Bacigalupo J., Johnson E.C., Vergara C, & Lisman J.E. (1991). Light-dependent channels from excised patches of Limulus ventral photoreceptors are opened by cGMP.. Proceedings of the National Academy of Sciences of the United States of America, 88(18), 7938-7942.

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