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Sp220i syringe pump

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The SP220I syringe pump is a precise and reliable laboratory instrument designed for fluid delivery applications. It features a microprocessor-controlled stepper motor that can accurately dispense liquid volumes from microliters to milliliters per minute. The pump can accommodate a wide range of syringe sizes and types, providing users with flexibility in their experimental setups.

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Syringe pump (26 citations)

3 protocols using sp220i syringe pump

1

Muscle Stem Cell Transplantation

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MuSCs were freshly isolated from donor mice and expanded in 12 well dishes in the presence of PFI-2 or DMSO (vehicle). After 7 days, expanded MuSCs were pelleted, resuspended in 30–50µl PBS and counted using a haemocytometer. Recipient mice received randomly via intramuscular injection, MuSCs into the TA. Where stated recipient mice also received a single injection of 0.15µg Notexin (NTX) and/or a non-lethal dose of 20Gy irradiation to the hindlimb legs 24 hours prior to transplantation. The injection was performed either by hand or with a Hamilton syringe controlled by an automated micropump (SP220I syringe pump, World Precision Instruments; Sarasota, FL).
Judson R.N., Quarta M., Oudhoff M.J., Soliman H., Yi L., Chang C.K., Lou G., Werff R.V., Cait A., Hamer M., Blonigan J., Paine P., Doan L.T., Groppa E., He W., Su L., Zhang R.H., Xu P., Eisner C., Low M., Barta I., Lewis C.A., Zaph C., Karimi M.M., Rando T.A, & Rossi F.M. (2018). Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential. Cell stem cell, 22(2), 177-190.e7.
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2

Transplantation of Muscle Stem Cells

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Recipient nude mice were pre-injured with Cardiotoxin under anesthesia 12 hours before transplantation. Mice received randomly via intramuscular injection 50 to 1000 MuSCs either in suspension, associated with viable muscle fibers, or associated with AMFs. Live MuSCs (expressing YFP, GFP or immunostained) were counted at a fluorescent microscope prior to transplantation. The injection was performed with a pulled transparent glass needle pre-coated with FBS, that was connected with silicon tubing, pre-loaded with mineral oil, to a Hamilton syringe. The syringe was controlled by an automated micropump (SP220I syringe pump, World Precision Instruments; Sarasota, FL). Cell suspension or individual fibers were carefully loaded in the needle under a microscope. A small opening of the skin was performed to expose the TA muscles. The needle was inserted into the proximal TA muscle and the cells or fibers were slowly injected into the muscle. The needle was left undisturbed for 5 minutes in the muscle. Before extracting the needle, the injection site was sealed with surgical glue (Tiessel, fibrin sealant, Baxter) to prevent leaking of cells or fibers. The skin wound was carefully sutured with one point stich (Coated Vicryl suture, 8-0, Ethicon).
Quarta M., Brett J.O., DiMarco R., De Morree A., Boutet S.C., Chacon R., Gibbons M.C., Garcia V.A., Su J., Shrager J.B., Heilshorn S, & Rando T.A. (2016). A bioengineered niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nature biotechnology, 34(7), 752-759.
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3

Transplantation of Muscle Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recipient nude mice were pre-injured with Cardiotoxin under anesthesia 12 hours before transplantation. Mice received randomly via intramuscular injection 50 to 1000 MuSCs either in suspension, associated with viable muscle fibers, or associated with AMFs. Live MuSCs (expressing YFP, GFP or immunostained) were counted at a fluorescent microscope prior to transplantation. The injection was performed with a pulled transparent glass needle pre-coated with FBS, that was connected with silicon tubing, pre-loaded with mineral oil, to a Hamilton syringe. The syringe was controlled by an automated micropump (SP220I syringe pump, World Precision Instruments; Sarasota, FL). Cell suspension or individual fibers were carefully loaded in the needle under a microscope. A small opening of the skin was performed to expose the TA muscles. The needle was inserted into the proximal TA muscle and the cells or fibers were slowly injected into the muscle. The needle was left undisturbed for 5 minutes in the muscle. Before extracting the needle, the injection site was sealed with surgical glue (Tiessel, fibrin sealant, Baxter) to prevent leaking of cells or fibers. The skin wound was carefully sutured with one point stich (Coated Vicryl suture, 8-0, Ethicon).
Quarta M., Brett J.O., DiMarco R., De Morree A., Boutet S.C., Chacon R., Gibbons M.C., Garcia V.A., Su J., Shrager J.B., Heilshorn S, & Rando T.A. (2016). A bioengineered niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nature biotechnology, 34(7), 752-759.
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