Abiprism 3730x1 genetic analyzer

Manufactured by Thermo Fisher Scientific

The ABIPrism® 3730x1 Genetic Analyzer is a high-performance DNA sequencing instrument designed for DNA analysis. It utilizes capillary electrophoresis technology to separate and detect fluorescently labeled DNA fragments, providing accurate and reproducible DNA sequencing data.

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Lab products found in correlation

Powerplex 18d kit, by Promega (2 mentions) Usinggenemapper v 4, by Thermo Fisher Scientific (2 mentions) Bigdye terminator v3.1 cycle sequencing ready reaction kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using abiprism 3730x1 genetic analyzer

STR DNA Profiling of Fibroblasts and iPSCs

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Patient fibroblasts and derived iPSC lines were sent to the Johns
Hopkins University Genetic Resources Core Facility for STR DNA profile analysis
using a Promega PowerPlex 18D Kit. The PCR product was electrophoresed on an ABI
Prism^® 3730x1 Genetic Analyzer and data was analyzed using
GeneMapper^® v 4.0 software (Applied Biosystems).

Li R., Pradhan M., Xu M., Baskfield A., Farkhondeh A., Cheng Y.S., Beers J., Zou J., Liu C., Might M., Rodems S, & Zheng W. (2018). Generation of an induced pluripotent stem cell line (TRNDi002-B) from a patient carrying compound heterozygous p.Q208X and p.G310G mutations in the NGLY1 gene. Stem cell research, 34, 101362.

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BRAF Exon 15 Mutation Screening

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Exon 15 of the BRAF gene was PCR amplified using genomic DNA from the tumor samples. The forward and reverse primers and the PCR conditions were as described previously [22 (link)]. We limited our search to exon 15 as the previously described mutations were mostly described in this exon. The PCR amplicons were confirmed on 2 % agarose gel and directly sequenced using the Big Dye terminator v3.1 cycle sequencing ready reaction kit (Applied Biosystems) and by ABI PRISM 3730X1, genetic analyzer (Applied Biosystems). Identified mutations were confirmed in both forward and reverse directions by an independent PCR amplification and sequencing reaction. For the confirmed mutations, matched normal tissue samples were further analyzed to check whether the mutations are somatic or germline in nature. The sequencing results were read against the BRAF gene (GeneBank No.: NM_004333.4).

Murugan A.K., Qasem E., Al-Hindi H., Shi Y, & Alzahrani A.S. (2016). Classical V600E and other non-hotspot BRAF mutations in adult differentiated thyroid cancer. Journal of Translational Medicine, 14, 204.

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STR DNA Profiling of Fibroblasts and iPSCs

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