Histochemical analyses were conducted on fresh leaf and stem sections according to standard staining procedures. Stains used were 0.1% Ruthenium red [27 ]; 1% Toluidine Blue [28 (link),29 ]; Methylene blue [30 (link)]; 0.01% Calcofluor white [31 (link)]; Fast green [32 (link)]; 0.25% Coomassie blue for proteins [33 (link)]; Sudan Black B [34 ] 10% Ferric chloride [27 ,35 ]; under UV light [36 ]; Safranin [37 (link),38 (link)]; Wagner’s and Dittmar reagent [39 (link)] and 0.01% Acridine orange [40 ].
Approximately 10 leaves and stem tissue were sectioned with an Oxford Vibratome into 150–200 μm thick sections, stained and viewed using a Nikon Eclipse 80i compound light microscope (Tokyo, Japan). Images were captured with the NIS-Elements imaging software package. Fluorescence microscopy was carried out using Nikon Eclipse 80i compound light microscope (Tokyo, Japan) equipped with a Nikon DS-Fil fluorescent camera with excitation and DM wavelengths of 330 nm and 400 nm, respectively.
Approximately 10 leaves and stem tissue were sectioned with an Oxford Vibratome into 150–200 μm thick sections, stained and viewed using a Nikon Eclipse 80i compound light microscope (Tokyo, Japan). Images were captured with the NIS-Elements imaging software package. Fluorescence microscopy was carried out using Nikon Eclipse 80i compound light microscope (Tokyo, Japan) equipped with a Nikon DS-Fil fluorescent camera with excitation and DM wavelengths of 330 nm and 400 nm, respectively.