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T aoc

Manufactured by Solarbio
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Sourced in China

The T-AOC is a laboratory equipment used to measure the total antioxidant capacity of a sample. It provides an analytical measurement of the cumulative capacity of all the antioxidants present in a sample to neutralize free radicals or other oxidative species.

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Lab products found in correlation

Tnf α, by Beyotime (2 mentions) Superoxide dismutase sod, by Solarbio (2 mentions) Interleukin 6 (il 6), by Abcam (2 mentions) Mcp 1, by Abcam (2 mentions) Lipopolysaccharides (lps), by Cusabio (2 mentions) Cerulein, by Merck Group (1 mentions) Malondialdehyde mda, by Solarbio (1 mentions) Resveratrol, by Merck Group (1 mentions) Glutathione (gsh), by Nanjing Jiancheng (1 mentions) Human gingival fibroblasts hgfs, by ScienCell (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Tb green premix ex taq 2 kit, by Takara Bio (1 mentions) Zn no3 2 6h2o, by Merck Group (1 mentions) 2 methylimidazole, by Merck Group (1 mentions) Murine macrophages raw 264.7 cell line, by American Type Culture Collection (1 mentions) Synergy 2 microplate reader, by Agilent Technologies (1 mentions) T aoc, by Beyotime (1 mentions) Gsh px, by NJJCBIO (1 mentions) Gsh px, by Solarbio (1 mentions)

6 protocols using t aoc

1

Resveratrol Modulates Inflammation and Oxidation

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Resveratrol (≥99%, Sigma, USA), sodium carboxymethyl cellulose (UNIVAL, Germany), cerulein (Sigma, USA), total cholesterol (TC) and total triglyceride (TG) content assay kits (Solarbio, China), LPS (cusabio, China), MCP-1 (Abcam, USA), TNF-α (Beyotime, China), IL-6 (Abcam, USA) malondialdehyde (MDA) (Solarbio, China), superoxide dismutase (SOD) (Solarbio, China), and total antioxidant capacity (T-AOC) (Solarbio, China) ELISA kits.
Zhang X., Yang G., Chen Y., Mu Z., Zhou H, & Zhang L. (2022). Resveratrol pre-treatment alleviated caerulein-induced acute pancreatitis in high-fat diet-feeding mice via suppressing the NF-κB proinflammatory signaling and improving the gut microbiota. BMC Complementary Medicine and Therapies, 22, 189.
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2

Proinflammatory and Antioxidant Markers in Mice

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Blood was collected from mice in each group and centrifuged at 15000 rpm for 10 min to obtain the serum. In the first part of experiments, the levels of proinflammatory cytokines including LPS (cusabio, China), MCP-1 (Abcam, USA), TNF-α (Beyotime, China), and IL-6 (Abcam) were determined by ELISA according to the manufacturer’s instructions. In the second part of experiments, the levels of malondialdehyde (MDA) (Solarbio, Beijing, China), superoxide dismutase (SOD) (Solarbio, Beijing, China), and total antioxidant capacity (T-AOC) (Solarbio, Beijing, China) were measured by ELISA according to the manufacturer’s instructions. The OD value of each well was immediately read at 450 nm.
Zhang X., Yang G., Chen Y., Mu Z., Zhou H, & Zhang L. (2022). Resveratrol pre-treatment alleviated caerulein-induced acute pancreatitis in high-fat diet-feeding mice via suppressing the NF-κB proinflammatory signaling and improving the gut microbiota. BMC Complementary Medicine and Therapies, 22, 189.
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3

Evaluating oxidative stress in HK-2 cells

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HK-2 cells were inoculated into six-well plates at 1 × 105/well. The cells were then digested with trypsin and collected. MDA (Nanjing Jiancheng, China, A003-2-2), T-AOC (Solarbio, Beijing, China, BC1310), and GSH (Nanjing Jiancheng, China, A006-2-1) levels were detected according to the instructions.
Ma Y., Zhou Q., Zhao P., Lv X., Gong C., Gao J, & Liu J. (2023). Effect of transferrin glycation induced by high glucose on HK-2 cells in vitro. Frontiers in Endocrinology, 13, 1009507.
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4

Synthesis and Biological Evaluation of Zinc-based Biomaterials

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No further purification was required as all chemicals were obtained from commercial suppliers. Zn (NO3) 2.6H2O and 2-Methylimidazole were obtained from Sigma (Aldrich, St. Louis, MO, United States). RIZOL was purchased acquired from Life Technologies (Carlsbad, CA, United States). Takara (Shiga, Japan) supply RNA-iso Plus, Prime Script RT reagent kit and TB Green Premix Ex Taq II kit. Kits for the detection of superoxide dismutase (SOD), catalase (CAT) and total antioxidant volume (T-AOC) were purchased from Solarbio (Beijing, China). In addition to human gingival fibroblasts (HGFs, ScienCell, SanDiego, CA, United States), murine macrophages (RAW 264.7 cell line), Escherichia coli (E. coli) ATCC 25922 and Staphylococcus aureus (S. aureus) ATCC 29213 were obtained from American Type Culture Collection (ATCC, Manassas, VA). Rutin hydrate, Calcian-AM/PI Double Staining Kit and all chemical reagents and other biological kits were obtained from Dalian Meilun Biotechnologies Co., Ltd. No additional purification of all reagents used.
Xia X., Song X., Li Y., Hou W., Lv H., Li F., Li Y., Liu J, & Li X. (2022). Antibacterial and anti-inflammatory ZIF-8@Rutin nanocomposite as an efficient agent for accelerating infected wound healing. Frontiers in Bioengineering and Biotechnology, 10, 1026743.
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5

Antioxidant Capacity Evaluation of ZLHXTY

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The antioxidant capacity was determined in serum samples using a total antioxidant capacity test kit (T-AOC) purchased from Solarbio, Beijing, China (Cat#BC1315) according to the manufacturer’s protocol. Four graded concentrations of ZLHXTY water extract, i.e., 0.07, 0.14, 0.28, and 0.42g per 1 ml of extraction solution, were also evaluated for their intrinsic antioxidant potential and all the samples were measured under BioTek Synergy 2 microplate reader at the absorbance of 593 nm.
Mazhar M., Yang G., Xu H., Liu Y., Liang P., Yang L., Spáčil R., Shen H., Zhang D., Ren W, & Yang S. (2023). Zhilong Huoxue Tongyu capsule attenuates intracerebral hemorrhage induced redox imbalance by modulation of Nrf2 signaling pathway. Frontiers in Pharmacology, 14, 1197433.
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6

Antioxidant Biomarker Quantification Protocol

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Malondialdehyde(MDA) levels, superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione peroxidase (GSH-Px) activity, and total antioxidant capacity (T-AOC) were detected using the MDA (Solarbio, Shanghai, China, Cat. No.A003-1), SOD (NJJCBIO, Nanjing, China, Cat. No.A001-3), CAT (A007-1-1, NJJCBIO, Nanjing, China, Cat. No.A007-1), GSH-Px (NJJCBIO, Nanjing, China, Cat. No.A005), and T-AOC (Beyotime, Shanghai, China, Cat. No.A015-3-1) assay kits, respectively. The working solution and the sample were mixed and added to 96-well plates, the absorbance of the samples was measured. Calculations were done as per the instructions.
He T., Sun P., Liu B., Wan S., Fang P., Chen J., Huang G, & Min W. (2022). Puffball spores improve wound healing in a diabetic rat model. Frontiers in Endocrinology, 13, 942549.
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