Cells were lysed in RIPA buffer containing protease inhibitors. Protein concentration was determined by Bio-Rad Protein Assay (Bio-Rad). Equivalent protein was loaded and resolved by SDS-PAGE. Gels were transferred onto PVDF membranes (Millipore) and immunoblotted using the following antibodies: anti-RalA (BD Transduction Laboratories), anti-GAPDH (Cell Signaling Technology - CST), anti-β-Actin (CST), anti-β -Tubulin (CST), anti-4E-BP1 (CST), anti-phospho-4E-BP1 (Thr37/46 – CST), anti-LC3B (CST), anti-Perilipin 3/Tip47 (Santa Cruz), anti-RalBP1 (Santa Cruz), anti-Vinculin (CST). Goat α-rabbit and goat α-mouse HRP secondary antibodies (Jackson ImmunoResearch Laboratories, Inc) were used for detection. Western blot images were taken on a ChemiDoc (Bio-Rad) using chemiluminescence detection with WesternBright ECL (Advansta). Where applicable, band pixel intensities were quantified using ImageJ.
Anti perilipin 3 tip47
Manufactured by Santa Cruz Biotechnology
Anti-Perilipin 3/Tip47 is an antibody developed by Santa Cruz Biotechnology for the detection and study of Perilipin 3/Tip47, a protein involved in lipid droplet formation and trafficking. The antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to identify and analyze the expression of Perilipin 3/Tip47 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti rala, by BD (2 mentions)
Anti ralbp1, by Santa Cruz Biotechnology (2 mentions)
Goat α rabbit and goat α mouse hrp secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Anti gapdh, by Cell Signaling Technology (2 mentions)
Protein assay, by Bio-Rad (2 mentions)
Chemidoc, by Bio-Rad (2 mentions)
Westernbright ecl, by Advansta (2 mentions)
2 protocols using anti perilipin 3 tip47
1
Western Blot Analysis of Cellular Proteins
2
Western Blot Analysis of Cellular Proteins
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Cells were lysed in RIPA buffer containing protease inhibitors. Protein concentration was determined by Bio-Rad Protein Assay (Bio-Rad). Equivalent protein was loaded and resolved by SDS-PAGE. Gels were transferred onto PVDF membranes (Millipore) and immunoblotted using the following antibodies: anti-RalA (BD Transduction Laboratories), anti-GAPDH (Cell Signaling Technology - CST), anti-β-Actin (CST), anti-β -Tubulin (CST), anti-4E-BP1 (CST), anti-phospho-4E-BP1 (Thr37/46 – CST), anti-LC3B (CST), anti-Perilipin 3/Tip47 (Santa Cruz), anti-RalBP1 (Santa Cruz), anti-Vinculin (CST). Goat α-rabbit and goat α-mouse HRP secondary antibodies (Jackson ImmunoResearch Laboratories, Inc) were used for detection. Western blot images were taken on a ChemiDoc (Bio-Rad) using chemiluminescence detection with WesternBright ECL (Advansta). Where applicable, band pixel intensities were quantified using ImageJ.
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