Fitc conjugated goat fab anti human igm

Manufactured by Southern Biotech

FITC conjugated goat (Fab') anti-human IgM is a laboratory reagent used for the detection and identification of human immunoglobulin M (IgM) in various immunoassays. It is a fragment antigen-binding (Fab') portion of a goat-derived antibody that has been conjugated with the fluorescent dye fluorescein isothiocyanate (FITC).

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Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions)

Close spelling variants of this product

Goat anti-human IgM Anti-IgM-FITC Anti-human IgM IgM-FITC

2 protocols using fitc conjugated goat fab anti human igm

Antibody Binding on Pig Endothelial Cells

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Heat-inactivated serum samples were diluted 1/10 and incubated at room temperature with endothelial cells from GTKO and WT pigs. Cells were washed and incubated with either FITC conjugated goat (Fab') anti-human IgM (Southern Biotech, Birmingham, AL) or IgG (γ-chain specific) (Sigma, St. Louis, MO). Cells were then washed, resuspended in FACS buffer and analyzed on a FACSCalibur Flow Cytometer (Becton Dickinson, San Diego, CA) using FlowJo software (Tree Star, Ashland, OR).

Hawthorne W.J., Salvaris E.J., Phillips P., Hawkes J., Liuwantara D., Burns H., Barlow H., Stewart A.B., Peirce S.B., Hu M., Lew A.M., Robson S.C., Nottle M.B., D'Apice A.J., O'Connell P.J, & Cowan P.J. (2014). Control of IBMIR in Neonatal Porcine Islet Xenotransplantation in Baboons. American Journal of Transplantation, 14(6), 1300-1309.

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Xenoantibody Levels in Baboon Sera

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Xenoantibody levels in the sera of recipient baboons were determined at 28 days after transplantation of genetically modified porcine NICC. Heat-inactivated baboon serum samples were diluted 1/10 and were incubated at room temperature with endothelial cells from both GTKO and wild type pigs. Cells were washed twice with cold FACS buffer and incubated with FITC conjugated goat (Fab’) anti-human IgM (Southern Biotech, Birmingham, AL) or FITC conjugated goat anti-human IgG (γ-chain specific) (Sigma, St. Louis, MO). The cells were washed, resuspended in cold FACS buffer and analyzed on a FACSCalibur Flow Cytometer (Becton Dickinson, San Diego, CA) using FlowJo software (Tree stars, Ashland, Oregon).

Chen Y., Stewart J., Gunthart M., Hawthorne W., Salvaris E., O’Connell P., Nottle M., d’Apice A., Cowan P, & Kearns-Jonker M. (2014). Xenoantibody Response to Porcine Islet Cell Transplantation using GTKO, CD55, CD59, and Fucosyl-Transferase Multiple Transgenic Donors. Xenotransplantation, 21(3), 244-253.

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