The feed wastewater characterization and reactor performance were monitored by wet analyses. For the suspended solid (TSS) parameter, standard methods (APHA, 1998) were applied. COD is one of the most important parameters in determining the degree of organic pollution in wastewaters. COD analyses were carried out using the microdigestion and titration method specified in the standard methods (SM 5220B) [20 ].
An HPLC (Shimadzu) equipped with a Diode-Array Detection (DAD) detector and LC-QTOF-MS/MS (Agilent 6530 Accurate Mass–ESI Interface, Santa Clara, CA, USA) was used for liquid chromatography analysis. To achieve the chromatographic separation, the Purospher Star RP-18 column (125 mm × 2.0 mm, particle size 5 µm) was supplied with a C18 guard column by Merck (Darmstadt, Germany). The analyses were performed in Positive Ionization mode with eluent A (acetonitrile-methanol (2:1)) and eluent B (ammonium acetate 5 mM at pH 4.7 (acetic acid)). The flow rate was selected as 0.3 mL/min, and the injection volume was determined as 10 µL. According to the selected method, the eluent gradient started from 5% and rose to 95% of eluent A in 5 min (was held for 4 min) and turned to the initial condition in 5 min.
An HPLC (Shimadzu) equipped with a Diode-Array Detection (DAD) detector and LC-QTOF-MS/MS (Agilent 6530 Accurate Mass–ESI Interface, Santa Clara, CA, USA) was used for liquid chromatography analysis. To achieve the chromatographic separation, the Purospher Star RP-18 column (125 mm × 2.0 mm, particle size 5 µm) was supplied with a C18 guard column by Merck (Darmstadt, Germany). The analyses were performed in Positive Ionization mode with eluent A (acetonitrile-methanol (2:1)) and eluent B (ammonium acetate 5 mM at pH 4.7 (acetic acid)). The flow rate was selected as 0.3 mL/min, and the injection volume was determined as 10 µL. According to the selected method, the eluent gradient started from 5% and rose to 95% of eluent A in 5 min (was held for 4 min) and turned to the initial condition in 5 min.