Fluoromax 4

Manufactured by Horiba

Sourced in Japan, United States, France, Germany, United Kingdom, China

The FluoroMax-4 is a high-performance, research-grade fluorescence spectrometer designed for a wide range of applications. It features advanced optics, high sensitivity, and user-friendly software for accurate and reliable fluorescence measurements.

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Lab products found in correlation

Escalab 250xi, by Thermo Fisher Scientific (10 mentions) Prism 6, by GraphPad (4 mentions) Jem 2100, by JEOL (4 mentions) Uv 1800 spectrophotometer, by Shimadzu (3 mentions) Infinite 200 pro, by Tecan (3 mentions) Picoharp 300, by PicoQuant (3 mentions) Pma165a n m, by PicoQuant (3 mentions) Pdl 800 b, by PicoQuant (3 mentions) D8 advance, by Bruker (3 mentions) Cary 5000 spectrophotometer, by Agilent Technologies (3 mentions) Lambda 750 uv vis spectrometer, by PerkinElmer (3 mentions) Nicolet 6700, by Thermo Fisher Scientific (3 mentions) Escalab 250, by Thermo Fisher Scientific (3 mentions) Prism 5, by GraphPad (2 mentions) Dfhbi 1t, by Horiba (2 mentions) Suprasil ultra micro cell, by Hellma (2 mentions) Jsm 6700f, by JEOL (2 mentions) Uv 3600 spectrophotometer, by Shimadzu (2 mentions) Spark plate reader, by Tecan (2 mentions) H 7650, by Hitachi (2 mentions)

Close spelling variants of this product

Fluoromax-4 spectrometer (50 citations) Fluoromax-4 spectrophotometer (45 citations) FluoroMax-4 fluorometer (40 citations) FluoroMax-4 fluorescence spectrometer (28 citations) FluoroMax 4 fluorescence spectrophotometer (11 citations) Jobin Yvon Fluoromax-4 (9 citations) Fluoromax-4 luminescence spectrometer (8 citations) FluoroMax 4 system (5 citations) JOBIN YVON Fluoromax-4 spectrometer (4 citations) Yvon fluoromax-4 spectrofluorimeter (3 citations)

420 protocols using fluoromax 4

Emission and Excitation Spectra of Metal Complexes

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Emission and excitation spectra in solution
for complexes 10 and 13–16 were recorded on a FluoroMax 4 (JY Horiba Inc.) spectrofluorometer
in a 1 cm quartz cuvette (freshly distilled tetrahydrofuran, concentration
ca. 10^–5 M, 298 K). The emission quantum yields
in solution were determined under oxygen-free conditions by a comparative
method using LED 365 nm as excitation source and Rhodamine 6G in ethanol
(Φ = 95%) as the reference dye.^{33 (link)} The steady-state emission and excitation spectra of complexes 1 and 5–17 in the solid state
at 298 K and at 77 K were measured on HR2000 (Ocean Optics), FluoroLog
3, and FluoroMax 4 (JY Horiba Inc.) spectrofluorometers. The xenon
lamps (300 and 450 W) served as excitation sources. The pulse laser
DTL-399QT “Laser-export Co. Ltd” (351 nm, 50 mW, pulse
width 6 ns, repetition rate 1 kHz), a monochromator MUM (LOMO, bandwidth
of slit 1 nm), photon counting head H10682 (Hamamatsu), and multiple-event
time digitizer P7887 (FAST ComTec GmbH) were used for lifetime measurements.
The samples were placed in a cryostat optCRYO 105 for measurements
in the temperature range 77–270 K. The absolute emission quantum
yields of solid samples, which were loaded in Teflon cuvettes and
covered by a quartz glass ring, were measured using a FluoroLog 3
(JY Horiba Inc.) spectrofluorometer and a Quanta-phi integration sphere
(Horiba).

Chakkaradhari G., Eskelinen T., Degbe C., Belyaev A., Melnikov A.S., Grachova E.V., Tunik S.P., Hirva P, & Koshevoy I.O. (2019). Oligophosphine-thiocyanate Copper(I) and Silver(I) Complexes and Their Borane Derivatives Showing Delayed Fluorescence. Inorganic Chemistry, 58(6), 3646-3660.

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Fluorescent Dye Binding Assay for Amyloid Proteins

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Recombinant Aβ₄₂, K18 tau, and αSyn were expressed and produced by E. coli as described previously [34 (link)–36 (link)]. The fluorescent dyes were dissolved in Milli-Q H₂O or dimethyl sulfoxide (DMSO) and further diluted in 1×PBS pH 7.4. The absorbance of the compounds was measured. Thioflavin T assays against Aβ₄₂ and K18 tau and αSyn fibrils were performed as described previously [34 (link), 36 (link)], with two independent experiments and three technical replicates (Fluoromax 4, Horiba Scientific, Japan). The dyes were then mixed with either 2 μL of αSyn, 5 μL of K18 tau (380 μg/ml) or 5 μL of Aβ₄₂ fibril (80 μg/ml) solution in a 45 μL quartz cuvette (quartz SUPRASIL Ultra Micro Cell, Hellma). The solutions were incubated for 1 min at room temperature and resuspended, and fluorescence was measured with a spectrofluorometer (FluoroMax-4, Horiba Jobin Yvon, Japan) using a known excitation wavelength for the ligands.

Straumann N., Combes B.F., Dean Ben X.L., Sternke-Hoffmann R., Gerez J.A., Dias I., Chen Z., Watts B., Rostami I., Shi K., Rominger A., Baumann C.R., Luo J., Noain D., Nitsch R.M., Okamura N., Razansky D, & Ni R. (2023). Visualizing alpha-synuclein and iron deposition in M83 mouse model of Parkinson’s disease in vivo. bioRxiv.

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Cellular Uptake and Endocytic Pathways

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For cellular uptake of CuS-NCs, A549-WT and A549-TRPV1 KD cells were seeded in plates (1.0 × 10⁶ cells/well) followed by the addition of CuS-NCs (0.1 mM Cu) and further incubation for 6, 12 and 24 h, respectively. Then, the cells were collected for cell counting and disruption under ultrasonication, and ICP-OES was used to determine the Cu amount. As to the cellular uptake of IS-Micelles in 4T1-Luc breast cancer cells, IS-Micelles and I-Micelles (10.0 μg mL⁻¹ ICG) were incubated with 4T1-Luc cells for 6, 12 and 24 h, respectively. Then, the amount of ICG was determined using the fluorescence spectrophotometer (Fluoromax-4, HORIBA) after extraction of ICG from the disrupted 4T1-Luc cells. For the endocytic pathway, the inhibitors including 10.0 µg·mL⁻¹ chlorpromazine, 100.0 µg·mL⁻¹ amiloride, 5.0 µg·mL⁻¹ nystatin were added into 4T1-Luc tumor cells (1.0 × 10⁶ cells/well) followed by 1 h incubation at 37 °C or 4 °C in serum-free RPMI 1640 medium^{39 (link)}. Then, IS-Micelles (10.0 μg mL⁻¹ ICG) were added into the medium for 2 h incubation. Afterward, the cells were collected through trypsin treatment, centrifugation, and lysis under ultrasonication. Finally, the amount of ICG was determined by the fluorescence spectrophotometer (Fluoromax-4, HORIBA).

Li T., Jiang S., Zhang Y., Luo J., Li M., Ke H., Deng Y., Yang T., Sun X, & Chen H. (2023). Nanoparticle-mediated TRPV1 channel blockade amplifies cancer thermo-immunotherapy via heat shock factor 1 modulation. Nature Communications, 14, 2498.

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Comprehensive Structural Characterization of Material

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The crystalline structure and composition were performed by X-ray diffraction analysis (XRD, Rigaku RINT-2000, Cu Kα radiation at 40 kV and 40 mA) and X-ray photoelectron spectroscopy (XPS, ESCALAB 250xi, Thermo Fisher Scientific). The elemental contents were tested by inductively coupled plasma optical emission spectroscopy (ICP-OES-720ES, Agilent, USA). The morphology was observed using field emission scanning electron microscopy (FE-SEM, Supra 55, Zeiss, Germany) and transmission electron microscopy (TEM, JEM-2100F, JEOL, Japan) systems. The cross-sectional SEM image was taken by argon-ion milling machine (GATAN, ILION693) with a voltage of 5 kV, following with SEM observation. The aberration-corrected high-angle annular dark-field scanning transmission electron microscopy was performed using JEM-ARM200F. UV-visible diffuse reflectance spectra were implemented on a UV-2600 (Shimadzu) spectrometer using BaSO₄ as the reference. Photoluminescence (PL) spectra were performed on a HORIBA Fluoromax-4 (HORIBA JY, HORIBA Fluoromax-4, USA) under laser excitation at 350 nm. The electron paramagnetic resonance (EPR) measurements were recorded using a JES-FA200 spectrometer at low temperature (−150 °C).

Gao R.T., Zhang J., Nakajima T., He J., Liu X., Zhang X., Wang L, & Wu L. (2023). Single-atomic-site platinum steers photogenerated charge carrier lifetime of hematite nanoflakes for photoelectrochemical water splitting. Nature Communications, 14, 2640.

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Fluorescent Intensity Analysis of PMs

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Fluorescein-loaded PMs and solutions of fluorescein of equivalent concentration (274 μM) were analysed for fluorescence intensity over a range of wavelengths (increment of 1 nm from 485 nm to 600 nm) using a FluoroMax-4 (Horiba Scientific, Stanmore, UK). Release of fluorescein into supernatant after storage was also measured using a FluoroMax-4 (Horiba Scientific, Stanmore, UK).

Scarpa E., Bailey J.L., Janeczek A.A., Stumpf P.S., Johnston A.H., Oreffo R.O., Woo Y.L., Cheong Y.C., Evans N.D, & Newman T.A. (2016). Quantification of intracellular payload release from polymersome nanoparticles. Scientific Reports, 6, 29460.

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Spectroscopic Characterization of Compounds

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The absorption spectra were recorded on a JASCO V-650 UV-Vis Spectrophotometer and the fluorescence emission spectra on a Horiba Jobin-Yvon Scientific Fluoromax-4. Spectra of solid samples were collected with a Horiba-Jobin-Yvon Fluoromax-4^® spectrofluorometer using an optic fibre connected to the equipment, by exciting the solid compounds at appropriated λ (nm). A correction for the absorbed light was performed when necessary. Lifetime studies were carried out on TemPro, Deltahub Nanoled of Horiba Jobin-Yvon, with a 455 nm Nanoled. All instruments were provided by Proteomass-BIOSCOPE facility.
The mass spectra were obtained using the Thermo Scientific mass spectrometer (ISQ LT Single Quadrupole Mass Spectrometer) by electron impact with ionizing electron energy of 70 eV.
The NMR spectra were recorded on a Bruker Avance spectrometer in DMSO-d₆ at 25 °C using glass ampoules: ¹H NMR spectrum at 500 MHz and ¹³C NMR spectrum at 126 MHz. An internal standard was used for tetramethylsilane (SiMe₄) or a signal of residual protons and carbon of ¹³C solvent with respect to SiMe₄ (for DMSO-d₆: δ_H 2.50 ppm, δ_C 39.5 ppm). The following abbreviations were used to describe the spectrum ¹H NMR: s—singlet, d—doublet, m—multiplet.
Melting points were measured on a Stuart melting point apparatus SMP30 and were uncorrected.

Pedro G., Duarte F., Cheptsov D.A., Volodin N.Y., Ivanov I.V., Santos H.M., Capelo-Martinez J.L., Cuerva C., Oliveira E., Traven V.F, & Lodeiro C. (2023). Exploring Coumarin-Based Boron Emissive Complexes as Temperature Thermometers in Polymer-Supported Materials. Sensors (Basel, Switzerland), 23(3), 1689.

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Amyloid Aggregation Monitoring by Nile Red

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Samples of ApCPEB PLD (5 μM in PBS, pH 7.0) in the presence or absence of 4:1 M excess of AmB and EGCG were removed at 100 min of incubation at 37 °C. A threefold excess of Nile Red was added, and the fluorescence spectrum was recorded using a Jobin Yvon Horiba FluoroMax 4 instrument (Horiba Jobin Yvon) and a 3-mm square quartz cuvette at 25.0 °C. Spectra were recorded in an emission wavelength range of 550–750 nm, with a 120 nm/min scan speed. Spectra of AmB and EGCG alone were also recorded and subtracted from the spectrum of the ApCPEB PLD complex. The excitation and emission wavelengths were calibrated using a fine Xe emission line and the Raman water peak, respectively.

Hervás R., del Carmen Fernández-Ramírez M., Galera-Prat A., Suzuki M., Nagai Y., Bruix M., Menéndez M., Laurents D.V, & Carrión-Vázquez M. (2021). Divergent CPEB prion-like domains reveal different assembly mechanisms for a generic amyloid-like fold. BMC Biology, 19, 43.

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Comprehensive Characterization of TiO2/CdS Composites

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The structure, morphology, specific surface areas, optical properties, and carrier migration and recombination of the samples were investigated using powder X-ray diffraction (XRD, XRD-6100, Shimadzu, Kyoto, Japan), field emission scanning electron microscope (FESEM, Regulus-8100, Tokyo, Japan), energy dispersive X-ray spectroscopy (EDX, Hitachi Limited, Tokyo, Japan), transmission electron microscopy (TEM, FEI TALOS 200S, Portland, Oregon, America), high-resolution TEM (HRTEM, FEI TALOS 200S, Portland, Oregon, America), specific surface area and porosity analyzer (micromeritics ASAP 2020, Atlanta, GA, USA), fluorescence spectrometer (PL, HORIBA Fluoromax-4, Kyoto, Japan), and electrochemical impedance spectroscopy (EIS, CHI600E, Shanghai Chenhua Instrument Co. Ltd., Shanghai, China); moreover, ultraviolet-visible spectrophotometer (TU-1901, Beijing Purkinje General Instrument Co. Ltd., Beijing, China) was employed to study the obtained TiO₂/CdS composites from a photocatalytic point of view.

Du Y.E., Niu X., He X., Hou K., Liu H, & Zhang C. (2021). Synthesis and Photocatalytic Activity of TiO2/CdS Nanocomposites with Co-Exposed Anatase Highly Reactive Facets. Molecules, 26(19), 6031.

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Weak-Affinity Protein Binding Assay

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To make the weak-affinity column, C-terminal His₆-tagged PMs or PC portal at 2 mg was applied to a 1-ml TALON Superflow and IMAC (Clontech) equilibrated in 20 mM Hepes (pH 7.5) and 70 mM KAc buffer. MV portal (without His₆ tag) at 2 mg was conjugated to NHS-activated agarose slurry (Pierce), as per the manufacturer’s instructions. The MV portal–conjugated beads were packed into 1-ml disposable syringe and washed with 20 mM Hepes (pH 7.5) and 70 mM KAc buffer. One hundred microliters of SP or CP was applied onto the columns at 0.2 mg/ml, and 250 μl fractions were collected. Ovalbumin at 0.2 mg/ml was used as a negative control for binding to PM or portal ring (PC portal or MV portal) weak-affinity columns. The tryptophan fluorescence emission of the fractions was measured on an AMINCO-Bowman AB2 spectrofluorometer or Horiba FluoroMAX 4 spectrofluorometer at an excitation wavelength of 295 nm and an emission wavelength of 340 nm, with bandpasses of 1 and 8, respectively. The emitted light in AMINCO-Bowman AB2 fluorometer was recorded in arbitrary units (A.U.), whereas Horiba FluoroMAX 4 spectrometer was measured in counts per second (CPS).

Motwani T., Lokareddy R.K., Dunbar C.A., Cortines J.R., Jarrold M.F., Cingolani G, & Teschke C.M. (2017). A viral scaffolding protein triggers portal ring oligomerization and incorporation during procapsid assembly. Science Advances, 3(7), e1700423.

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Photophysical Characterization of Compounds

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UV/vis absorption spectra were recorded on the Shimadzu UV-1800 Spectrophotometer (Shimadzu, Duisburg, Germany) using quartz cells with 1 cm path length at room temperature. Emission spectra were measured on the Horiba FluoroMax-4 (HORIBA Ltd., Kyoto, Japan) at room temperature using quartz cells with 1 cm path length. The fluorescence quantum yield of the target compounds in solution and solid state were measured by using the Integrating Sphere Quanta-φ of the Horiba-Fluoromax-4. Time-resolved fluorescence measurements were carried out using time-correlated single-photon counting (TCSPC) with a nanosecond LED (λ = 370 nm).

Kopotilova A.E., Moshkina T.N., Nosova E.V., Lipunova G.N., Starnovskaya E.S., Kopchuk D.S., Kim G.A., Gaviko V.S., Slepukhin P.A, & Charushin V.N. (2023). 3-Aryl-5-aminobiphenyl Substituted [1,2,4]triazolo[4,3-c]quinazolines: Synthesis and Photophysical Properties. Molecules, 28(4), 1937.

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