Log In Sign up
The largest database of trusted experimental protocols

Mouse cytokine elisa plate array

Manufactured by Signosis
Visit Supplier

The Mouse Cytokine ELISA Plate Array is a laboratory equipment designed for the simultaneous detection and quantification of multiple mouse cytokines in a single sample. It provides a reliable and efficient method for analyzing the expression levels of various cytokines in mouse-based research applications.

Automatically generated - may contain errors

Lab products found in correlation

Mouse tgf β1 duoset elisa development system, by R&D Systems (1 mentions) Golgistop, by BD (1 mentions) Golgiplug, by BD (1 mentions) Anti fitc microbeads, by Miltenyi Biotec (1 mentions) Cytofix cytoperm plus kit, by BD (1 mentions)

6 protocols using mouse cytokine elisa plate array

1

Purification and Stimulation of CD4+ T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4+T cells were purified from splenic single cell suspensions using FITC-conjugated monoclonal antibody to CD4 molecule (GK 1.5) and anti-FITC microbeads (Miltenyi Biotec K.K., Tokyo, Japan). Purified CD4+T cells (2.5 × 105/200 µl) were stimulated with 5 µg/mL anti-CD3 + 2.5 µg/mL anti-CD28 in the presence of GolgiPlug and GolgiStop (BD PharMingen Co. Ltd.) for 5 h. Cells were fixed and permeabilized with the Cytofix/Cytoperm Plus kit (BD Biosciences Co. Ltd.) according to the manufacturer’s directions. Cells were stained with FITC or PE-conjugated monoclonal Ab against each cytokine. Purified CD4+T cells (2.5 × 105/200 µl) were also stimulated with 5 µg/mL anti-CD3+2.5 µg/mL anti-CD28 for 48 h, and culture supernatants were analyzed for cytokine production using the Mouse Cytokine ELISA Plate Array (Signosis, Inc., Santa Clara, CA) according to the manufacturer’s directions. Samples in 96-well microtiter plates were analyzed on a LAS3000 (FujiFilm Ltd., Tokyo, Japan) and the chemiluminescent intensity of each sample was evaluated by Image Gauge ver. 4.0 (FUJIFILM Software, Yokohama, Japan). The ratios of each sample to a blank of intensity per mm3 were obtained. The production of TGF-β was analyzed with a mouse TGF-β1 DuoSet ELISA Development system (R&D Systems, Inc., Minneapolis, MN).
Miyake K., Adachi K., Watanabe M., Sasatomi Y., Ogahara S., Abe Y., Ito K., Dan Justin Y.K., Saito T., Nakashima H, & Hamano S. (2014). Parasites alter the pathological phenotype of lupus nephritis. Autoimmunity, 47(8), 538-547.
+ Open protocol
+ Expand
2

Mouse Cytokine ELISA Profiling in AD

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mouse cytokine ELISA plate array was purchased from Signosis (Santa Clara, CA). The AD/ApoE2 and AD/ApoE4 mice (6 mice/genotype) were euthanized at 6 months of age and their brains were harvested and homogenized. Three sets of pooled homogenates were created per genotypic group (pool 2 brain homogenates x3). Applied each pooled sample to one ELISA plate (therefore, 3 plates per genotypic group), and the analysis was performed as per manufacturer's instructions. The cytokine values were corrected to the blank and the average level of each cytokine was plotted for the two groups.
Dorey E., Bamji-Mirza M., Najem D., Li Y., Liu H., Callaghan D., Walker D., Lue L.F., Stanimirovic D, & Zhang W. (2017). Apolipoprotein E Isoforms Differentially Regulate Alzheimer's Disease and Amyloid-β-Induced Inflammatory Response in vivo and in vitro. Journal of Alzheimer's disease : JAD, 57(4).
+ Open protocol
+ Expand
3

Screening Microglia Cytokine Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols
The secreted cytokines from primary microglia 18 hrs after α-syn PFF with either PBS or NLY01 treatment were screened using a mouse cytokine ELISA plate array (Signosis, Santa Clara, CA) according to the manufacturer instructions.
Yun S.P., Kam T.I., Panicker N., Kim S., Oh Y., Park J.S., Kwon S.H., Park Y.J., Karuppagounder S.S., Park H., Kim S., Oh N., Kim N.A., Lee S., Brahmachari S., Mao X., Lee J.H., Kumar M., An D., Kang S.U., Lee Y., Lee K.C., Na D.H., Kim D., Lee S.H., Roschke V.V., Liddelow S.A., Mari Z., Barres B.A., Dawson V.L., Lee S., Dawson T.M, & Ko H.S. (2018). Block of A1 astrocyte conversion by microglia is neuroprotective in models of Parkinson’s disease. Nature medicine, 24(7), 931-938.
+ Open protocol
+ Expand
4

Screening Microglia Cytokine Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols
The secreted cytokines from primary microglia 18 hrs after α-syn PFF with either PBS or NLY01 treatment were screened using a mouse cytokine ELISA plate array (Signosis, Santa Clara, CA) according to the manufacturer instructions.
Yun S.P., Kam T.I., Panicker N., Kim S., Oh Y., Park J.S., Kwon S.H., Park Y.J., Karuppagounder S.S., Park H., Kim S., Oh N., Kim N.A., Lee S., Brahmachari S., Mao X., Lee J.H., Kumar M., An D., Kang S.U., Lee Y., Lee K.C., Na D.H., Kim D., Lee S.H., Roschke V.V., Liddelow S.A., Mari Z., Barres B.A., Dawson V.L., Lee S., Dawson T.M, & Ko H.S. (2018). Block of A1 astrocyte conversion by microglia is neuroprotective in models of Parkinson’s disease. Nature medicine, 24(7), 931-938.
+ Open protocol
+ Expand
5

Histamine Promotes Intrahepatic Biliary Proliferation

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Alterations in intrahepatic biliary mass (IBDM) are a hallmark feature of PSC and since our studies focus on the role of cholangiocytes during liver damage, we evaluated the role of histamine on the promotion of IBDM and biliary proliferation by semi-quantitative immunohistochemistry and analysis for CK-19 (biliary-specific marker) and Ki-67, respectively 2 (link), 13 (link).
Liver inflammation was determined by qPCR in total liver samples for tumor necrosis factor (TNF)-α. Additionally, the number of Kupffer cells in our animal groups was evaluated by F4/80 immunohistochemistry and semi-quantitative analysis using the Visiopharm VIS 2017.2 software (Broomfield, CO). Furthermore, we evaluated the serum levels of various pro-inflammatory mediators using the Mouse Cytokine ELISA Plate Array (Signosis, Inc., Santa Clara, CA). Since histamine may interact through downstream interleukin signaling, we measured the expression of IL-6 by immunohistochemistry in our animal models.
Kennedy L., Meadows V., Demieville J., Hargrove L., Virani S., Glaser S., Zhou T., Rinehart E., Jaeger V., Kyritsi K., Pham L., Alpini G, & Francis H. (2020). Biliary damage and liver fibrosis are ameliorated in a novel mouse model lacking l-histidine decarboxylase/histamine signaling. Laboratory investigation; a journal of technical methods and pathology, 100(6), 837-848.
+ Open protocol
+ Expand
6

Histamine Promotes Intrahepatic Biliary Proliferation

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Alterations in intrahepatic biliary mass (IBDM) are a hallmark feature of PSC and since our studies focus on the role of cholangiocytes during liver damage, we evaluated the role of histamine on the promotion of IBDM and biliary proliferation by semi-quantitative immunohistochemistry and analysis for CK-19 (biliary-specific marker) and Ki-67, respectively 2 (link), 13 (link).
Liver inflammation was determined by qPCR in total liver samples for tumor necrosis factor (TNF)-α. Additionally, the number of Kupffer cells in our animal groups was evaluated by F4/80 immunohistochemistry and semi-quantitative analysis using the Visiopharm VIS 2017.2 software (Broomfield, CO). Furthermore, we evaluated the serum levels of various pro-inflammatory mediators using the Mouse Cytokine ELISA Plate Array (Signosis, Inc., Santa Clara, CA). Since histamine may interact through downstream interleukin signaling, we measured the expression of IL-6 by immunohistochemistry in our animal models.
Kennedy L., Meadows V., Demieville J., Hargrove L., Virani S., Glaser S., Zhou T., Rinehart E., Jaeger V., Kyritsi K., Pham L., Alpini G, & Francis H. (2020). Biliary damage and liver fibrosis are ameliorated in a novel mouse model lacking l-histidine decarboxylase/histamine signaling. Laboratory investigation; a journal of technical methods and pathology, 100(6), 837-848.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!