Csu 10 spinning disk head

Manufactured by Hamamatsu Photonics

The CSU-10 spinning disk head is a key component of confocal microscopy systems. It consists of a spinning disk with thousands of pinholes that rapidly scans a sample, allowing for high-speed, low-phototoxicity imaging of live cells and tissues.

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Lab products found in correlation

Axio observer, by Zeiss (1 mentions) C9100 13 emccd camera, by PerkinElmer (1 mentions) Axioskop 2 mot, by Zeiss (1 mentions) Orca flash4.0 v2 scmos camera, by Hamamatsu Photonics (1 mentions) Ti e microscope, by Yokogawa (1 mentions) Imageem emccd camera, by Hamamatsu Photonics (1 mentions)

2 protocols using csu 10 spinning disk head

Worm Immobilization for Confocal Imaging

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Worms were immobilized with 40 mM levamisole or 0.1 μm diameter polystyrene microspheres in water (Polysciences 00876‐15, 2.5% w/v suspension) on 10% agarose inwater pads. Unless noted otherwise, imaging was performed on a spinning disc confocal system (WaveFX system from Quorum Technologies), consisting of an inverted Zeiss AxioObserver equipped with a 100× oil (N.A. 1.4) objective, Yokogawa CSU-10 spinning disk head, 491 & 561 nm lasers, and a Hamamatsu C9100‐13 EMCCD camera, controlled by Volocity software version 6.5.1 (PerkinElmer). Body-length imaging was completed on a Zeiss Axioskop 2 mot with a 100× oil (N.A. 1.3) objective, FluoArc/HBO103 illuminator, via a Hamamatsu Orca ER C4742‐80 CCD camera and Open Lab (V5) (Agilent) software.

Chrystal P.W., Lambacher N.J., Doucette L.P., Bellingham J., Schiff E.R., Noel N.C., Li C., Tsiropoulou S., Casey G.A., Zhai Y., Nadolski N.J., Majumder M.H., Tagoe J., D’Esposito F., Cordeiro M.F., Downes S., Clayton-Smith J., Ellingford J., Mahroo O.A., Hocking J.C., Cheetham M.E., Webster A.R., Jansen G., Blacque O.E., Allison W.T., Au P.Y., MacDonald I.M., Arno G, & Leroux M.R. (2022). The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for vision. Nature Communications, 13, 6595.

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Visualizing cortical and embryonic microtubule networks

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Gravid adults were dissected directly on polylysine-coated
coverslips in a drop of egg buffer and were gently flattened by mounting
with 22.8 μm beads (Whitehouse scientific, Chester, UK) as spacers.
Cortical images were acquired using a Nikon Ti-E microscope equipped with a
100X, 1.49 NA objective; a Yokogawa CSU-X1 spinning disk head; and a
Hamamatsu ImageEM EM-CCD camera. Embryo cross-section images were acquired
using a Nikon TE-2000 microscope equipped with a 60X, 1.4 NA objective; a
Yokogawa CSU-10 spinning disk head; and a Hamamatsu Orca-Flash4.0
V2+ scMOS camera. For embryo imaging, mNG was excited using a 40 mW,
514 nm laser, which produced less autofluorescent background and
phototoxicity compared to 488 nm. GFP was excited using a 50 mW, 488 nm
laser and mKate2 was excited using a 50 mW, 561 nm laser.

Dickinson D.J., Schwager F., Pintard L., Gotta M, & Goldstein B. (2017). A single-cell biochemistry approach reveals PAR complex dynamics during cell polarization. Developmental cell, 42(4), 416-434.e11.

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