The femurs were separated and soaked in 4% formalin for at least 48 h, and subsequently used for scanning by micro-computed tomography (CT) and further bone density quantification using the Scanco viva CT 80 at a voxel size of 9 μm with an energy of 55 kV and 145 μA. Cortical and trabecular bone structures near the growth plate of the distal femur were reconstructed after the scan. We chose to reconstruct 1 mm centripetal from the growth plate and quantified cortical and trabecular bone volume by Scanco viva CT 80.
Vivact80
Manufactured by Scanco
Sourced in Switzerland
The VivaCT80 is a high-resolution micro-computed tomography (micro-CT) imaging system designed for in vivo and ex vivo applications. It provides non-invasive, three-dimensional imaging of small animal models and samples.
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Lab products found in correlation
μct evaluation program v6, by Scanco (6 mentions)
Vivact 40, by Scanco (4 mentions)
Ct ray v4.0 4, by Scanco (3 mentions)
Ct evaluation program v6, by Scanco (2 mentions)
Mimics research v19, by Materialise (2 mentions)
A1r mp, by Nikon (2 mentions)
Na edta, by Wuhan Servicebio Technology (2 mentions)
22g needle, by BD (2 mentions)
Tecnai g2 f20, by Thermo Fisher Scientific (2 mentions)
S 4800, by Hitachi (2 mentions)
Amira 3d 2021, by Thermo Fisher Scientific (1 mentions)
Ab93876, by Abcam (1 mentions)
As014, by ABclonal (1 mentions)
Mimics 17, by Materialise (1 mentions)
Axis ultra dld, by Shimadzu (1 mentions)
Tecnai g2 t20, by Thermo Fisher Scientific (1 mentions)
Nicolet 6700, by Thermo Fisher Scientific (1 mentions)
Inveon research workplace 4, by Siemens (1 mentions)
Vivact 80 scanner, by Scanco (1 mentions)
Multifocus, by Hologic (1 mentions)
139 protocols using vivact80
1
Femur Micro-CT Bone Density Analysis
2
Quantifying Abdominal Adiposity in Rats
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Abdominal visceral adiposity parameters of the live animals, including total abdominal fat (TAF), visceral abdominal fat (VAF), and subcutaneous abdominal fat (SAF) volume, were measured non-invasively at 12 weeks using three-dimensional (3D) Scanco VivaCT 80 scanner (Scanco Medical AG, Bassersdorf, Switzerland), following the manufacturer’s instructions.
During the scanning process, the rats were anesthetized with 1% isoflurane (inhalation) and positioned on their back with their face up and head toward the front. Both hind limbs were extended and secured to a specimen holder, forming a 90° angle between the femur and spine, with the legs fully extended. The scanning area covered the region between the proximal end of the L1 and the distal end of the L6 lumbar vertebrae. Scanning was performed with an isotropic voxel size of 18 μm, using parameters of 70 kVp energy, 114 μA intensity, 31.9 mm field of view (FOV)/diameter, and 200 ms integration time. The scanning energy and voxel size were determined based on optimizing scanning time, tissue detail, and minimizing radiation exposure, following the manufacturer’s guidelines.
The acquired micro-CT scans were processed using the imaging software (Scanco Medical AG, vivaCT 80, Bassersdorf, Switzerland), and the TAF, VAF, and SAF were analyzed using the generated 3D reconstructed images.
During the scanning process, the rats were anesthetized with 1% isoflurane (inhalation) and positioned on their back with their face up and head toward the front. Both hind limbs were extended and secured to a specimen holder, forming a 90° angle between the femur and spine, with the legs fully extended. The scanning area covered the region between the proximal end of the L1 and the distal end of the L6 lumbar vertebrae. Scanning was performed with an isotropic voxel size of 18 μm, using parameters of 70 kVp energy, 114 μA intensity, 31.9 mm field of view (FOV)/diameter, and 200 ms integration time. The scanning energy and voxel size were determined based on optimizing scanning time, tissue detail, and minimizing radiation exposure, following the manufacturer’s guidelines.
The acquired micro-CT scans were processed using the imaging software (Scanco Medical AG, vivaCT 80, Bassersdorf, Switzerland), and the TAF, VAF, and SAF were analyzed using the generated 3D reconstructed images.
3
High-Resolution Microstructural Analysis via μCT
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For the method part, the samples were scanned with a μCT (Viva CT 80; Scanco Medical AG, Brüttisellen, Switzerland) operated at 70 kVp, 114 μA, 8-W, 31.9 mm FOV, 1500 projections, and an integration time of 500 ms. The data sets were reconstructed into three-dimensional (3D) volumes with an isotropic nominal resolution of 10.4 μm voxel size.
For the application part, the samples were scanned with a μCT 50 (Viva CT 80; Scanco Medical AG, Brüttisellen, Switzerland) operated at 70 kVp, 76 μA, 300–900 ms integration time and 9-to-10 μm voxel size.
For the application part, the samples were scanned with a μCT 50 (Viva CT 80; Scanco Medical AG, Brüttisellen, Switzerland) operated at 70 kVp, 76 μA, 300–900 ms integration time and 9-to-10 μm voxel size.
4
Rat Bone Structural Analysis via μCT
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The complete left femur of the rats was collected, and the muscle and connective tissues were peeled off before analysis. The structural features of the trabecular bone of the proximal tibia and distal femur were determined by a high-resolution micro-computed tomography (μCT) scanner (VivaCT 80, Scanco, Zurich, Switzerland) according to the manufacturer’s instructions. The experimental conditions were as follows: voxel size, 18 μm (70 kvp, 114 μm, integration time = 200 ms); and FOV/diameter, 31.9 mm. Moreover, the biomechanical properties of the left femur were determined by the μCT analysis software (VivaCT 80, Scanco).
5
Fracture Callus Characterization Protocol
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Fracture calluses were assessed as previously described [25 (link)]. Samples were dissected 21 days after injury (from mice used for the behavioral experiments) and fixed in 10% Zn-formalin at room temperature for 5 days. Micro-computed tomography (microCT) analysis was conducted using a Scanco vivaCT 80 (Scanco Medical, Brüttisellen, Switzerland) at a scan resolution of 8 μm. Calluses were scanned 1 mm proximal and 1 mm distal from the fracture site and assessed for total volume (TV) and bone volume (BV) in mm3, and bone mineral density (BMD) in mg HA/mm3. Fixed fracture calluses were decalcified using 12% EDTA pH 7.4, cleared of EDTA, and embedded into paraffin. Sections were cut at a thickness of 5 μm and stained using safranin-O/fast-green to visualize bone and cartilage. A minimum of five sections was used to conduct computer-assisted histomorphometry analysis, and results were presented as an amount relative to the total area of the fracture callus.
6
Murine Bone Microarchitecture Analysis
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Tibial and lumbar vertebral samples from 24-week-old mice were scanned using a Scanco viva CT80 (Scanco Medical AG, Switzerland) operated with the following parameters: voxel size of 10.4 μm, energy at 55 kVp, intensity of 145 μA, FOV/Diameter of 31.9 mm, and 300 msec integration time. The measurements were quantitatively analyzed. Three-dimensional reconstructions were made using Scanco software. The region of interest for histomorphometric parameters was selected in the 1 mm region starting 0.2 mm below the growth plate.
7
Micro-CT Analysis of Knee Samples
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Knee samples were fixed in 3.5% formafix for 24 h. Afterwards, micro-CT scanning was carried out using Scanco vivaCT 80 (SCANCO Medical, Brüttisellen, Switzerland) with a voxel size of 15.6 µm at 70 kVp, 113 µA and 400 ms integration time. µCT Ray V4.0-4 (Scanco Medical AG, Brüttisellen, Switzerland) and imageJ were used to generate the representative images. For subchondral trabecular bone and osteophyte evaluation, the volume-of-interest (VOI) was defined by manual contouring and calculated using the μCT Evaluation Program V6.6 (Scanco Medical AG, Brüttisellen, Switzerland). Data were reported according to the guidelines for tissue imaging by the American Society of Bone and Mineral Research45 (link).
8
Micro-CT Analysis of Spinal Microarchitecture
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The samples were fixed in 4% paraformaldehyde (PFA) for 24 h. To assess alterations in the microarchitecture of the surgically modeled segment, micro-CT (Scanco Viva-CT80, Scanco Medical AG, Basserdorf, Switzerland) was then used to determine morphometric indices, such as intervertebral disc height and percent bone volume (BV/TV), from the volume of interest (VOI), with the resolution of 11.6 μm, 70kVp, and 114μA. And the three-dimensional (3D) images of the CEP were collected by built-in software.
9
Micro-CT Analysis of Ankle Bone Volume
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Right ankle joints were fixed in 10% formalin for 48 h, washed in phosphate-buffered saline (PBS) for 2 h and then soaked in 75% ethanol, scanned by micro-CT system (Scanco VIVA CT80, SCANCO Medical AG, Switzerland). The scanning parameters were as follows: pixel size 15.6 μm, tube voltage 55 kV, tube current 72 μA, integration time 200 ms. The cross-section images were then reconstructed and realigned in 3D, the bone volume (BV) of astragalus were measured and a density threshold was set from 370 to 1000 as Bone by μCT Evaluation program V6.6 (Scanco Medical AG, Switzerland). A stack of 340–441 cross-sections was reconstructed, with an inter slice distance of 1 pixel (15.6 μm), corresponding to a reconstructed height of 5.3–6.9 mm, recreating the ankle joints.
10
Fracture Callus Analysis by μCT and Histomorphometry
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Fracture calluses were harvested at the indicated time point post‐fracture and fixed in 10% Zn‐formalin at room temperature for 5 days. μCT analysis was conducted using a Scanco vivaCT 80 (Scanco Medical) at a scan resolution of 8 μm. Calluses were scanned 1 mm proximal and 1 mm distal from the fracture site and assessed for TV and BV in mm3, and ratio of BV‐to‐TV (BV/TV). Fixed fracture calluses were decalcified using 12% EDTA, pH 7.4, cleared of EDTA, and embedded in paraffin. Sections were cut at a thickness of 5 μm and stained using Safranin‐O/Fast Green to visualize bone and cartilage. A minimum of five sections were used to conduct computer‐assisted histomorphometry analysis, and results are presented as an amount relative to the total area of the fracture callus.
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