R-HL60 cells were established from parental HL60 cells (ATCC, Rockville, MD, USA) and were characterized as described previously [1 (link)]. HL60 and R-HL60 cells were cultured in RPMI 1640 medium (Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Amarillo, TX, USA) and 1% antibiotic-antimycotic reagents (Gibco). Cells were cultured at an incubator with 5% CO2 at a temperature of 37 °C, and the culture medium was changed every 2–3 days. Cytarabine, bortezomib, carfilzomib, and marizomib were purchased from Sigma Aldrich (St. Louis, MO, USA). R-HL60 cells were exposed to different concentrations of Cytarabine, bortezomib, carfilzomib, and marizomib, and the cell viability was measured using the CCK-8 assay (Sigma Aldrich, St. Louis, MO, USA) All data were compared with the vehicle control (ddH2O for Cytarabine treatment; dimethyl sulfoxide [DMSO] for bortezomib, carfilzomib, and marizomib treatment).
Cytarabine
Manufactured by Merck Group
Sourced in United States, Germany, Ireland, United Kingdom
Cytarabine is a synthetic nucleoside analog used as a chemotherapeutic agent. It is a key component in the treatment of various types of cancer, including acute myeloid leukemia, acute lymphoblastic leukemia, and non-Hodgkin's lymphoma.
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Lab products found in correlation
Doxorubicin, by Merck Group (8 mentions)
Daunorubicin, by Merck Group (8 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Methotrexate, by Merck Group (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Papain, by Thermo Fisher Scientific (4 mentions)
Idarubicin, by Merck Group (3 mentions)
Dmem f12, by Thermo Fisher Scientific (3 mentions)
Vincristine, by Merck Group (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Poly l lysine (pll), by Merck Group (3 mentions)
Paclitaxel, by Merck Group (3 mentions)
Etoposide, by Merck Group (3 mentions)
L glutamine, by Biosera (3 mentions)
Azacytidine, by Merck Group (3 mentions)
Poly d lysine, by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Neurobasal plus medium, by Thermo Fisher Scientific (3 mentions)
95 protocols using cytarabine
1
Characterization and Drug Response of R-HL60 Cells
2
Cytarabine-Induced AML Cell Death
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The human AML cell lines U937 and HL60 were obtained from American Type Culture Collection (ATCC). These cell lines were cultured in RPMI 1640 medium conditions similar to those for primary AML cells. For cytarabine treatment, cells were seeded with a density of 5×105 cells/ml one day before the experiment, and fresh medium was added together with different concentrations of cytarabine according to experimental design, with or without 30 μM chloroquine (Sigma-Aldrich).
3
Hippocampal Neuron Isolation and Transduction
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The neonatal mice within 24 h were sterilized with 75% medical alcohol, after which the brain of the mice was cut out and the hippocampal tissue was quickly separated, and cut into small pieces. The hippocampal tissue pieces were digested with 0.125% trypsin for 15 min at 37°C, ground and centrifuged. Cells were seeded in 10-mm dishes coated with polylysine (10 mmol/L) at a density of 1×106 cells/mL and maintained in Neural Basal Medium (Gibco, Carlsbad, CA, USA) with 2% B27 (Gibco) and 0.25% Glumax (Gibco). After 3 days, 2.5 μg/mL cytarabine (Sigma-Aldrich) was added to the medium for 24 h to suppress the proliferation of glial cells. Next, 50% of the medium was renewed every three days. Cells were cultured at 37°C with 5% CO2 for 14 days before experimentations [23 (link)].
Hippocampal neurons were then transduced with lentivirus (Genechem; multiplicity of infection=5) carrying sh-NC, sh-MALAT1-1, sh-MALAT1-2, sh-MALAT1-3, oe-NC, oe-MALAT1, sh-METTL3-1, sh-METTL3-2, sh-METTL3-3, and oe-METTL3. After 48 h, cells were harvested for subsequent experimentations.
Hippocampal neurons were then transduced with lentivirus (Genechem; multiplicity of infection=5) carrying sh-NC, sh-MALAT1-1, sh-MALAT1-2, sh-MALAT1-3, oe-NC, oe-MALAT1, sh-METTL3-1, sh-METTL3-2, sh-METTL3-3, and oe-METTL3. After 48 h, cells were harvested for subsequent experimentations.
4
Synergistic Anti-Cancer Drug Combination
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Triptolide (TPL, C20H24O6, MW: 360.40), cytarabine (araC, C9H13N3O5, MW: 243.22), doxorubicin (ADM, C27H29NO11, MW: 543.52), and N-acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). TPL was dissolved in dimethyl sulfoxide (Sigma-Aldrich, Dorset, UK) as a 100 μM stock solution, and was freshly diluted in culture medium before use. cytarabine, doxorubicin, were dissolved in phosphate-buffered saline (PBS) as a 100 mM stock solution at -20°C. Light exposure was kept to a minimum for all drugs used.
5
Cytarabine Metabolism Quantification
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Cytarabine, 3,4,5,6‐tetrahydrouridine, and NBMPR were purchased from Sigma‐Aldrich (St. Louis, MO). [13C,15N2]‐Cytarabine was purchased from Alsa Chim (Illkirch Graffenstaden, France). All other chemicals were purchased from Thermo Fisher Scientific (Waltham, MA) unless otherwise specified.
6
Preparation and Characterization of Sodium Alginate Solutions
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Two percent sodium alginate was prepared by dissolving sodium alginate (Sigma-Aldrich) in phosphate-buffered saline (PBS) and sterilizing by heating to 80°C in a water bath for 15 min (Leo et al., 1990 (link)). Anti-CD150-PE, anti-GRPC5C Alexa Fluor-405, and anti-Ki67-Alexa Fluor 405 antibodies for flow cytometry were purchased from R&D Systems. Anti-CD49f-PE and anti-CD90-VioBlue were purchased from Miltenyi Biotec. Transforming growth factor beta-1 (TGFβ-1) and fibroblast growth factor were obtained from PeproTech. Fms-like tyrosine kinase-3 (Flt-3), G-CSF, stem cell factor (SCF), and interferon alpha were purchased from ImmunoTools. All-trans retinoic acid (ATRA) was obtained from Sigma-Aldrich. Hoechst 33342, propidium iodide (PI), and pyronin Y (PY) were purchased from Sigma-Aldrich. The CountBrightTM Absolute Counting Beads was purchased from Thermo Fisher Scientific. MHY1485, AZD8055, rosiglitazone, KU-55933, LEE011, roscovitine (seliciclib, CYC202), glasdegib (PF-04449913), sodium butyrate, dasatinib, BIO, plerixafor (AMD3100), quizartinib, and sorafenib were purchased from Selleckchem. Adiponectin, triglitazone LE135, PD169316, harmine, nilotinib, ethylisopropyl amiloride, anisomycin, and curcumin were purchased from Sigma-Aldrich, while prostaglandin E2 was from BioVision/Cambridge Bioscience. Cytarabine and daunorubicin were purchased from Sigma-Aldrich.
7
Multicompound Cancer Treatment Preparation
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Three-drug Daunorubicin (DNR), Cytarabine (Ara-C) and Idarubicin (IDR) (Sigma-Aldrich St. Louis, MO, USA) were dissolved in purified water and make a stoke for treat cell lines.
8
Selenium Supplementation in Neuronal Cells
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2 mg/mL cytarabine (Sigma, USA) was supplemented into the culture medium after 24h. Then, the cell monolayers were washed three times with PBS (0.1 M, pH 7.4) and incubated in neurobasal culture medium at 37°C with 5% CO2 humidified atmosphere. After being cultured for 48h, neurons were respectively cultured in 2 mL fresh complete medium and incubated in the presence of 0 (Control), 10−9 (Se-I), 10−8 (Se-II), 10−7 (Se-III), 10−6 (Se-IV) or 10−5 (Se-V) mol/L of Se as Na2SeO3 for 0 h, 3 h, 6 h, 12 h, 24 h and 48 h.
9
Culturing mouse neuroblastoma and rat cortical neurons
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Mouse neuroblastoma N2a cells were grown in Dulbecco modified Eagle's medium (DMEM) (Hyclone, Logan, UT, USA) supplemented with 10%(V/V) fetal bovine serum (Gibco, Grand Island, NY, USA) at 37 °C with 5% CO2 in a humid incubator. Cerebral cortex neurons were prepared from neonatal rats within 72 h of birth. Cerebral cortex neurons were isolated as described previously (Zhu et al., 2016 ). Briefly, the cortices were digested with DMEM containing 2 mg mL−1 papain (Invitrogen, Carlsbad, CA, USA) and 50 mg mL−1 DNase (Sigma‐Aldrich, St. Louis, MO, USA) for 50 min at 37 °C. Digested tissues were filtered with a 70 μm nylon cell strainer (Corning, Corning, NY, USA). Isolated cells were seeded in 0.05 mg mL−1 poly‐L‐lysine (Solarbio, Beijing, China)‐coated plates at a density of 2 × 105 cells per well in a 24‐well plate. Neurons were cultured in DMEM/F12 (Gibco) containing 1% B27 serum‐free supplement (Invitrogen), and 1% penicillin/streptomycin (Gibco). After 48 h, 10 mm cytarabine (Sigma‐Aldrich) was added to suppress the growth of glial cells. Experimental treatments were performed after 7 days of culture.
10
Primary Olfactory Bulb Neuron Culture
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Primary olfactory bulb cultures were prepared from E16.5 embryos. Olfactory bulb pieces were incubated in 0.05% of Trypsin-EDTA (Gibco) for 6 min at 37°C. Trypsination was stopped by DMEM-F12 containing 10% of FCS. Afterward, tissue was dissociated in HBSS and centrifuged for 2 min at 500 × g. The pellet was resuspended in primary growth medium (PGM), and cells were plated at 1 × 105 per cm2 on poly-d-lysine-coated cell culture plates. We maintained cells in Primary Neuron Growth Medium BulletKit (PNGM, Lonza) at 37°C, 5% of CO2, and relative humidity of 98%. To inhibit glial proliferation, we added cytarabine (Sigma, 1 μM = AraC) and maintained cultures for 14–18 days in vitro.
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