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Dryslide

Manufactured by BD
Sourced in Canada

The DrySlide is a laboratory equipment product designed for slide preparation and storage. It provides a controlled environment for maintaining the integrity of microscope slides during transport and storage. The DrySlide features a compact and durable construction to protect slides from external factors.

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2 protocols using dryslide

1

Detecting and Typing Vancomycin-Resistant Enterococci

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Bacti-swabs (Remel, Lenexa, KS) were used by trained research personnel to obtain patient and environmental samples. Patients’ nares, oral cavity, groin, and perianal site swabs were cultured on bile-esculin plates with 6 mg/L vancomycin (BEV6) on the same day. Hands and environmental swabs (bed controls, side table top and bottom, nurse call button, curtain, toilet seat, door knob, TV remote control, wheelchair, and other equipment when available) were enriched overnight in Brain Heart Infusion broth at 36ºC before culturing on BEV6 plates. Growth suggestive of VRE was confirmed by pyrrolidonyl arylamidase testing (DrySlide; BD, Franklin Lakes, NJ).
Pulsed-field gel electrophoresis was performed to determine the relatedness of VRE isolates. Genomic deoxyribonucleic acid was prepared and digested with SmaI (New England BioLabs, Beverly, MA) using a previously described method [14 (link)]. SmaI fragments were separated using a CHEF DR III apparatus (Bio-Rad, Hercules, CA) and compared using BioNumerics software (Applied Maths, Kortrijk, Belgium). Similarity of isolates was calculated using Dice coefficient (BioNumerics software). Isolates were placed in the same pulsotype if their restriction patterns were ≥80% similar [14 (link)].
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2

Bacteriologic Analysis of Bird Tissues

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Liver and lung tissue from all birds were submitted for bacteriologic analysis. For birds with gross lesions, tissues from affected organs also were submitted. All fresh samples were cultured aerobically on blood agar (comprised of tryptic soy agar [BD, Mississauga, Canada] and 6% sheep blood) and MacConkey II agar (BD) at 35 C for 24-48 h. Bacterial colonies were identified by selective growth, colony morphology, Gram stain, and biochemical characteristics. Samples from the first five carcasses were screened using API 20E test strips (BioMerieux, Marcy l'Etoile, France). All subsequent isolates were identified based on a combination of catalase, oxidase (Dryslide; BD), indole (Kovac's), and sugar fermentation activity (TSI Slant; Oxoid, Nepean, Canada). Lyophilized isolates were sent in Aimes transport medium to the US Geological Survey-National Wildlife Health Center in Madison, Wisconsin, US for somatic serotyping according to the Heddleston scheme (Heddleston et al. 1972) .
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