Pulsed-field gel electrophoresis was performed to determine the relatedness of VRE isolates. Genomic deoxyribonucleic acid was prepared and digested with SmaI (New England BioLabs, Beverly, MA) using a previously described method [14 (link)]. SmaI fragments were separated using a CHEF DR III apparatus (Bio-Rad, Hercules, CA) and compared using BioNumerics software (Applied Maths, Kortrijk, Belgium). Similarity of isolates was calculated using Dice coefficient (BioNumerics software). Isolates were placed in the same pulsotype if their restriction patterns were ≥80% similar [14 (link)].
Dryslide
The DrySlide is a laboratory equipment product designed for slide preparation and storage. It provides a controlled environment for maintaining the integrity of microscope slides during transport and storage. The DrySlide features a compact and durable construction to protect slides from external factors.
2 protocols using dryslide
Detecting and Typing Vancomycin-Resistant Enterococci
Pulsed-field gel electrophoresis was performed to determine the relatedness of VRE isolates. Genomic deoxyribonucleic acid was prepared and digested with SmaI (New England BioLabs, Beverly, MA) using a previously described method [14 (link)]. SmaI fragments were separated using a CHEF DR III apparatus (Bio-Rad, Hercules, CA) and compared using BioNumerics software (Applied Maths, Kortrijk, Belgium). Similarity of isolates was calculated using Dice coefficient (BioNumerics software). Isolates were placed in the same pulsotype if their restriction patterns were ≥80% similar [14 (link)].
Bacteriologic Analysis of Bird Tissues
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