Gatan microscopy suite software

Cryogenic transmission electron microscopy (cryo-TEM) images were obtained using a Tecnai F20 X TWIN microscope (FEI Company, Hillsboro, OR, USA) equipped with a field emission gun, operating at an acceleration voltage of 200 kV. Images were recorded on the Gatan Rio 16 CMOS 4k camera (Gatan Inc., Pleasanton, CA, USA) and processed with Gatan Microscopy Suite (GMS) software (Gatan Inc., Pleasanton, CA, USA). The specimen preparation was done by vitrification of the aqueous solutions on grids with holey carbon film (Quantifoil R 2/2; Quantifoil Micro Tools GmbH, Großlöbichau, Germany). Prior to use, the grids were activated for 15 s in oxygen plasma using a Femto plasma cleaner (Diener Electronic, Ebhausen, Germany). Cryo-samples were prepared by applying a droplet (3 μL) of the suspension to the grid, blotting with filter paper, and immediately freezing in liquid ethane using a fully automated blotting device Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). After preparation, the vitrified specimens were kept under liquid nitrogen until they were inserted into a cryo-TEM-holder Gatan 626 (Gatan Inc., Pleasanton, CA, USA) and analyzed in the TEM at −178 °C.

The TEM imaging of the silica marker was performed in R1 and R2 solutions before spraying them, as well as in solutions obtained after the fourth step of filter extraction (R1a-c ex4 and R2a-c ex4, respectively). The analyzed material was prepared via the cryogenic fixation of the tested samples (cryo-TEM). For the purpose of the research, a Tecnai F20 X-TWIN microscope (FEI Company, Hillsboro, OR, USA) was used. The microscope was equipped with a field emission gun operated at an acceleration voltage of 200 kV. The images were recorded on a Gatan Rio 16 CMOS 4k camera and processed with Gatan Microscopy Suite (GMS) software (Gatan Inc., Pleasanton, CA, USA). Specimen preparation was performed via vitrification of the aqueous solutions on grids with a holey carbon film. Before use, the grids were activated for 15 s in oxygen plasma using a Femto plasma cleaner. Cryo-samples were prepared by applying a droplet (about 3 μL) of the suspension to the grid, blotting with filter paper, and immediately freezing in liquid ethane using a fully automated blotting device. After preparation, the vitrified specimens were kept under liquid nitrogen until they were inserted into a cryo-TEM and analyzed in the TEM at −178 °C.

The morphology of the freeze-dried MS was observed using a scanning electron microscope (SEM; FEI Company, Hillsboro, OR, USA; Quanta 250 FEG). Powder samples were stuck to the microscopic stubs by the double-sided adhesive carbon type. The micrographs were obtained under low vacuum (80 Pa) with an acceleration voltage 5 kV from secondary electrons collected by a Large Field Detector (LFD). The size and size distribution of the MS was evaluated using ImageJ 1.45 s software.
The morphology of the micelles was analyzed using a transmission electron microscope (TEM; Tecnai F20 TWIN, FEI Company, Hillsboro, OR, USA) equipped with a field emission gun (200 kV). Images were recorded on the Gatan Rio 16 CMOS 4k camera (Gatan Inc., Pleasanton, CA, USA) and processed with Gatan Microscopy Suite (GMS) software (Gatan Inc., Pleasanton, CA, USA). The lyophilized micelles were dissolved in deionized water (5 mg/mL) and a drop of the solution was placed on a copper grid covered with carbon film. The samples were observed after negative staining followed by air-drying at RT.

Cryogenic Transmission Electron Microscopy (cryo-TEM) images were collected with a Tecnai F20 X TWIN microscope (FEI Company, Hillsboro, OR, USA) equipped with a field emission gun operating at an acceleration voltage of 200 kV. Images were recorded on the Gatan Rio 16 CMOS 4 k camera and processed with Gatan Microscopy Suite (GMS) software (Gatan Inc., Pleasanton, CA, USA). Specimen preparation was done by the vitrification of the aqueous solutions on grids with holey carbon film (Quantifoil R 2/2; Quantifoil Micro Tools GmbH, Großlöbichau, Germany). Prior to use, the grids were treated for 15 s in oxygen plasma using a Femto plasma cleaner (Diener Electronic, Ebhausen, Germany). Cryo-samples were prepared by applying a droplet (3 μL) of the suspension to the grid, blotting with filter paper, and immediate freezing in liquid ethane using a fully automated blotting device Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). The vitrified specimens were kept under liquid nitrogen prior the insertion into a cryo-TEMholder Gatan 626 (Gatan Inc., Pleasanton, CA, USA) and analyzed at −178 °C.

Cryo-TEM images were obtained using a Tecnai F20 X TWIN microscope (FEI Company, Hillsboro, OR, USA) equipped with a field emission gun and operating at an acceleration voltage of 200 kV. Images were recorded on a Gatan Rio 16 CMOS 4k camera (Gatan Inc., Pleasanton, CA, USA) and processed with Gatan Microscopy Suite (GMS) software (version 3.31.2360.0, Gatan Inc., Pleasanton, CA, USA). Specimen preparation was performed by vitrification of the aqueous solutions on grids with holey carbon film (Quantifoil R 2/2; Quantifoil Micro Tools GmbH, Großlöbichau, Germany). Prior to use, the grids were activated for 15 s in oxygen plasma using a Femto plasma cleaner (Diener Electronic, Ebhausen, Germany). Cryo-samples were prepared by applying a droplet (3 μL) of the suspension to the grid, blotting it with filter paper, and immediately freezing in liquid ethane using a fully automated Vitrobot Mark IV blotting device (Thermo Fisher Scientific, Waltham, MA, USA). After preparation, the vitrified specimens were kept under liquid nitrogen until they were inserted into a cryo-TEM-holder Gatan 626 (Gatan Inc., Pleasanton, CA, USA) and analyzed in the TEM at −178 °C.

Cryogenic Transmission Electron Microscopy (cryo-TEM) images were obtained using a Tecnai F20 X TWIN microscope (FEI Company, Hillsboro, OR, USA) equipped with a field emission gun, operating at an acceleration voltage of 200 kV. Images were recorded on the Gatan Rio 16 CMOS 4 k camera (Gatan Inc., Pleasanton, CA, USA) and processed with Gatan Microscopy Suite (GMS) software (Gatan Inc., Pleasanton, CA, USA). Specimen preparation was done by vitrification of the aqueous solutions on grids with holey carbon film (Quantifoil R 2/2; Quantifoil Micro Tools GmbH, Großlöbichau, Germany). Prior to use, the grids were activated for 15 s in oxygen plasma using a Femto plasma cleaner (Diener Electronic, Ebhausen, Germany). Cryo-samples were prepared by applying a droplet (3 μL) of the suspension to the grid, blotting with filter paper and immediate freezing in liquid ethane using a fully automated blotting device Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA). After preparation, the vitrified specimens were kept under liquid nitrogen until they were inserted into a cryo-TEM-holder Gatan 626 (Gatan Inc., Pleasanton, CA, USA) and analyzed in the TEM at −178 °C.