Milli q pure water system

Manufactured by Merck Group

Sourced in United States

The Milli-Q pure water system is a laboratory equipment manufactured by Merck Group. It is designed to produce high-quality, ultrapure water by purifying and deionizing tap water. The system utilizes a combination of filtration and ion exchange technologies to remove impurities, resulting in water that meets the stringent requirements for various laboratory applications.

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8 protocols using milli q pure water system

Liquid Chromatography-Mass Spectrometry Analysis

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Liquid chromatography (LC) system (AB Sciex Jasper HPLC) was coupled with mass spectrometer (MS) system (AB Sciex Triple Quad 4500). AB Sciex Jasper HPLC consisted of a binary pump, an autosampler, a column oven, and a controller. AB Sciex Triple Quad 4500MD (Applied Biosystems Sciex, Ontario, Canada) was operated in positive ion mode with electrospray ion (ESI) source, using multiple reaction monitoring (MRM). Analyst software version 1.6.3 (Sciex, Toronto, Canada) was used for system control, data acquisition, and processing.
Electronic balance (MettlerToledoXP26, d = 0.001 mg, Switzerland), vortex oscillator (Ql-901, Jiangsu, China), high-speed low-temperature centrifuge (Eppendorf5417c, Germany), and Milli-Q pure water system (Millipore, USA) were also used.

Zheng Y., Xu N., Hu X., Zhang Q., Liu Y, & Zhao Q. (2020). Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry, 2020, 8838219.

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Analytical Method for Abexinostat and Givinostat

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Abexinostat (Fig. 1A) and givinostat (used as internal standard, IS, Fig. 1B) were provided by Shanghai Chuangsai Technology Co., Ltd. (Shanghai, China) with > 98% purity. The methanol and acetonitrile used in the experimental procedure were both HPLC grade, provided by Merck Company (Darm-stadt, Germany). Formic acid provided by Anaqua Chemicals Supply (ACS, American) also was HPLC grade. Milli-Q pure water system was used to prepare ultrapure water (Millipore, Bedford, USA).

Yu Y., Hu J., Chen X., Wu H.L., Wang A, & Tang C. (2024). Development of an UPLC-MS/MS method for quantitative analysis of abexinostat levels in rat plasma and application of pharmacokinetics. BMC Chemistry, 18(1), 37.

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Moxidectin Formulation Development

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MXD (moxidectin, ≥95.5%) was donated by Xinyu Pharmaceutical Co., Ltd. (Suzhou, China). Poloxamer 407 and Poloxamer 188 were purchased from Guangzhou Ruixin Chemical Industry Co., Ltd. (Guangzhou, China). Polyoxyethylene (20) Oleyl Ether (Brij^® 98, CAS:9004-98-2) was produced by Sigma-Aldrich (Shanghai, China). All the solvents and reagents used in the extraction procedure were of HPLC grade or analytical grade. Ultra-pure water was obtained from a Milli-Q pure water system (Millipore, Burlington, MA, USA).

Ruan X., Hu J., Lu L., Wang Y., Tang C., Liu F., Gao X., Zhang L., Wu H., Huang X, & Wei Q. (2022). Poloxamer 407/188 Binary Thermosensitive Gel as a Moxidectin Delivery System: In Vitro Release and In Vivo Evaluation. Molecules, 27(10), 3063.

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Optimized Proteomics Sample Preparation

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Tetrahydrofuran (THF), acetic acid, o-dichlorobenzene (o-DCB), n-BuOH, 1,3,5-tri-(4-aminophenyl)benzene (TAPB), 2,5-dimethoxyterephthalaldehyde (DMTA), bovine serum albumin (BSA), cytochrome c (Cyt c), trypsin, ammonium formate, ammonium acetate, ammonium bicarbonate (NH₄HCO₃), urea, dithiothreitol (DTT) and iodoacetamide (IAA) were ordered from Sigma-Aldrich (St. Louis, MO). The standard peptide (with sequence of SLHTLFGELCK) was synthesized by Jietai biotechnology Co. Ltd. (Shanghai, China). HPLC grade acetonitrile (ACN) was from Merck Life Science Pvt. Ltd. (Worli, Mumbai, India). Formic acid (FA) was obtained from Acros Organics (Geel, Belgium). The mesoporous MCM-41 was purchased from Nankai catalyst factory (Tianjin, China). GELoader tips were from Eppendorf AG (Hamburg, Germany). Deionized water was purified by Milli-Q pure water system (Millipore, Bedford, MA, USA).

Zhang X., Qing G., Yu L., Kang H., Chen C., Li X, & Liang X. (2018). Novel nanoporous covalent organic frameworks for the selective extraction of endogenous peptides. RSC Advances, 8(65), 37528-37533.

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HPLC Analysis of Azobenzene Derivatives

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MB (C₁₆H₁₈ClN₃S, 98%) and AZB (C₁₅H₁₆ClN₃S, 98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). AZA (C₁₄H₁₄ClN₃S, 80%) and AZC (C₁₃H₁₂ClN₃S, 50%) were purchased from West Asia Chemical Industry Co. Ltd. (Shandong, China). Methanol and acetonitrile (HPLC grade) were obtained from J. T. Baker Chemical Co. (Phillipsburg, NJ, USA). Polytetrafluoroethylene (PTFE, 0.22 µm) filters were supplied by Branch Billion Lung Experimental Equipment Co., Ltd. (Tianjin, China). Ammonium acetate (HPLC grade) was obtained from Honeywell (Morris Plains, NJ, USA). Formic acid (HPLC grade) was supplied by Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The ultrapure water was produced using Milli-Q Pure Water System (Millipore, Billerica, MA, USA). Alumina-neutral (ALN) solid-phase extraction (SPE) cartridges (3 mL/500 mg) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrophilic–lipophilic balanced (HLB) cartridges (6 mL/200 mg) were supplied by Waters (Taunton, MA, USA). The columns were Sunfire C₁₈ column (150 mm × 2.1 mm, 5 µm, Waters Technologies Ltd., Wexford, Ireland), Symmetry C₁₈ column (100 mm × 2.1 mm,3.5 µm, Waters Technologies Ltd., Wexford, Ireland), Hypersil ODS C₁₈ column (150 mm × 2.1 mm, 5 μm, Thermo Fisher Scientific Ltd., Bartlesville, OK, USA), and Capcell Pak C₁₈ column (100 mm × 2 mm, 5 µm, Shiseido Ltd., Tokyo, Japan).

Zhang X., Hui Y., Fang C., Wang Y., Han F., Lou X., Fodjo E.K., Cai Y, & Kong C. (2021). Determination of Methylene Blue and Its Metabolite Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry. Molecules, 26(16), 4975.

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Synthesis of Functionalized TiO2 Nanoparticles

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TiO₂ nanoparticles (anatase, >99.9%), 3-amino-propyltrimethoxysilane (APTMS), LA, and FA were purchased from Aladdin (Shanghai, China) and used as received. Toluene was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and was freshly distilled before use. Other reagents were of analytical grade and used without further purification. Experimental water was prepared by a Millipore Milli-Q pure water system.

Zhou S., Lei M., Bai J., Liu X., Wu L., Long M., Huang K, & Yin D. (2022). Titania Nanotubes-Bonded Sulfamic Acid as an Efficient Heterogeneous Catalyst for the Synthesis of n-Butyl Levulinate. Frontiers in Chemistry, 10, 894965.

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Characterization of Nanomaterials via Spectroscopy

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The UV-1800 ultraviolet–visible light spectrophotometer was purchased from Shimadzu Instrument Equipment Co., Ltd. (Tokyo, Japan) The transmission electron microscope (Philips TECNAI G2F20 company), CHI660E electrochemical workstation, working glassy carbon electrode (Φ = 3 mm), reference Ag/AgCl electrode, counter platinum wire electrode was all purchased from Shanghai Chenhua Instrument Co., Ltd. (Shanghai, China) The ultrasonic cleaner was obtained from Kunshan ultrasonic equipment Co., Ltd. (Kunshan, China), while the electric blast drying oven was purchased from Shanghai Yiheng Science Equipment Co., Ltd. (Shanghai, China). An ultrapure water preparation machine was used Millipore Milli-Q pure water system. (Shanghai Yarong Biochemical Equipment and Apparatus Co., Ltd., Shanghai, China)

Peng D., Lu D, & Pang G. (2020). Study on FcγRn Electrochemical Receptor Sensor and Its Kinetics. Molecules, 25(14), 3206.

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Prenatal Rat Euthanasia Study

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All animal studies, containing rats euthanasia procedures, were approved and complied with AALAC and IACUC guidelines and guidelines for institutional animal care in Shanghai Jiao Tong University (Permit number: A2016077). Sixteen SD rats weighing 400-500 g within 18 days of gestation were bought from IACUC of Shanghai Jiao Tong University.
All rats did not carry viruses, bacteria and parasites. Animal health were continuously recorded by a sentinel program. Rats were kept in Individual ventilated plastic cages (420mm X 250mm X 230mm). A standard cardbox house was provided, autoclaved hay (about 8 12 g/cage) and two Nestlets™ (about 5 × 5 cm) were supplied. Rats were fed a pelleted rat diet by Jiangsu cooperative medical bio-engineering co..LTD and ordinary pure water after high temperature sterilization. The rats were housed in an environment with temperature of 23±2°C, relative humidity of 50 ±15% and a light/dark cycle of 12/12hr(approximately 20 lx in the cage), the air pressure was controlled at 10 Pa. HPLC(High Performance Liquid Chromatography)-grade acetonitrile and formic acid were purchased from Merck (USA), calibration solution for TOF-MS was bought from AB SCIEX (AB SCIEX,Foster City, USA), ultra-pure water was generated by a Milli-Q pure water system (Millipore,USA).

Jin Y., Yu W., Hu X., Meng F., Luo Q., Chen Z., & Yang Z. (2021). Sevoflurane Exposure of Clinical Dose in Pregnant Rat Induces Vcan Changes Without Significant Neural Apoptosis in the Offspring.

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