BMSCs were seeded in a 24-well plate and cultured in osteogenic differentiation (OB) medium. After 14 days, the cells were fixed with 4% paraformaldehyde for 30 minutes, washed with phosphate-buffered saline (PBS) three times, and stained with alizarin red working solution (Sigma-Aldrich) for five minutes. After washing with PBS three times, images were captured with a light microscope.
Alizarin red working solution
Manufactured by Merck Group
Sourced in United States
Alizarin red working solution is a laboratory reagent used for the detection and identification of calcium in biological and chemical samples. It provides a colorimetric reaction, resulting in a red-orange color, when it interacts with calcium ions. The solution is prepared from the dye alizarin red S and is commonly used in various analytical techniques, such as histology and mineralogy, to visualize the presence and distribution of calcium-containing compounds.
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Lab products found in correlation
2 protocols using alizarin red working solution
1
Osteogenic Differentiation of BMSCs
2
Multilineage Differentiation of MSCs
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P3 MSCs were collected and added to a 6-well plate with a cell count of 1 × 105 per well. After 48 h, the medium was removed, and osteogenesis induction medium, chondrogenesis induction medium, and lipogenesis induction medium (the ingredients in Additional file 1 : Tables 2–4) were added to each well, respectively. After 21 days, the media were removed and MSCs were washed by PBS. MSCs were fixed with 4% paraformaldehyde for 30 min and stained with Alizarin Red working solution (Sigma-Aldrich, Inc, St Louis, MO, US) at room temperature for 30 min for osteogenesis induction. MSCs were treated with 1% hydrochloric acid for 5 min and stained with Alcian Blue working solution (Sigma-Aldrich, Inc, St Louis, MO, US) at room temperature for 30 min for chondrogenesis induction. MSCs were fixed with 4% paraformaldehyde for 30 min and stained with Oil Red O working solution (Sigma-Aldrich, Inc, St Louis, MO, US) at room temperature for 30 min for lipogenesis induction.
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