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Fty720

Manufactured by Cayman Chemical
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Sourced in United States, United Kingdom

FTY720 is a white, crystalline solid used in research applications. It is a synthetic compound with a molecular formula of C22H33N3O2. FTY720 is used as a research tool to study cellular processes and signaling pathways.

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Lab products found in correlation

Sphingosine 1 phosphate (s1p), by Cayman Chemical (5 mentions) Fty720 p, by Cayman Chemical (5 mentions) Fty720 phosphate, by Cayman Chemical (4 mentions) Sphingosine 1 phosphate (s1p), by Merck Group (4 mentions) Bromodeoxyuridine (brdu), by Merck Group (4 mentions) Flumist quadrivalent, by Sanofi (2 mentions) Fluzone quadrivalent, by Sanofi (2 mentions) αpd l1 10f 9g2, by BioXCell (2 mentions) Rmp1 14, by BioXCell (2 mentions) Sh rf 177, by Cayman Chemical (2 mentions) Sh rf 39, by Cayman Chemical (2 mentions) Fty720 p e, by Cayman Chemical (2 mentions) Sew2871, by Cayman Chemical (2 mentions) Isotype control, by BioXCell (2 mentions) Appropriate isotype control ab, by BioXCell (2 mentions) Anti cd122 ab clone tm β1, by BioXCell (2 mentions) Il 7 receptor blocking antibody clone a7r34, by BioXCell (2 mentions) Rat igg2a, by BioXCell (2 mentions) Depo provera, by Merck Group (2 mentions) Thedotap, by Merck Group (2 mentions)

154 protocols using fty720

1

Immunotherapy Protocol for Tumor Regression

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200 μg of αPD-1 (RMP1.14, BioXCell) or αPD-L1 (10F.9G2, BioXcell) diluted in saline were injected IP on days 7, 10, and 12 following orthotopic tumor implantation. For prolonged treatments, mice received 2 injections per week for 3 weeks also starting at day 7 post orthotopic tumor implantation. For FTY720 experiments, mice were injected with 0.5 mg/kg FTY720 (Cayman Chemical) diluted and administered according to manufacturer’s instructions in 1:1 solution of ethanol:PBS immediately prior to injection.
Burrack A.L., Spartz E.J., Raynor J.F., Wang I., Olson M, & Stromnes I.M. (2019). Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell reports, 28(8), 2140-2155.e6.
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2

Evaluating T Cell Infiltration and Tumor Uptake

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To evaluate the role of infiltrating T cells in the tumor uptake of 68Ga-grazytracer, MC38 tumor–bearing C57BL/6 mice were intraperitoneally injected with 25 μg of FTY-720 (Cayman Chemical) on day –1 and continuous administration of FTY-720 (10 μg daily) was performed from day 0 to day 12. Mice were also treated with three doses of anti–PD-1 (clone RMP1-14; BioXcell; 200 μg × 3) plus anti–CTLA-4 (clone 9D9; BioXcell; 200 μg × 3) antibodies on days 0, 3, and 6. On day 0 (baseline) and day 6, mice were subjected to small-animal PET imaging of 68Ga-grazytracer or 18F-FDG at 0.5 or 1 h postinjection. On day 6, five mice from each group were euthanized, and the tumor tissues were harvested. Each tumor was digested to obtain single-cell suspensions and subsequently sorted for NK1.1, CD4, and CD8 by flow cytometry as described below.
Zhou H., Wang Y., Xu H., Shen X., Zhang T., Zhou X., Zeng Y., Li K., Zhang L., Zhu H., Yang X., Li N., Yang Z, & Liu Z. (2022). Noninvasive interrogation of CD8+ T cell effector function for monitoring early tumor responses to immunotherapy. The Journal of Clinical Investigation, 132(16), e161065.
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3

Immunotherapy Protocol for Tumor Regression

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200 μg of αPD-1 (RMP1.14, BioXCell) or αPD-L1 (10F.9G2, BioXcell) diluted in saline were injected IP on days 7, 10, and 12 following orthotopic tumor implantation. For prolonged treatments, mice received 2 injections per week for 3 weeks also starting at day 7 post orthotopic tumor implantation. For FTY720 experiments, mice were injected with 0.5 mg/kg FTY720 (Cayman Chemical) diluted and administered according to manufacturer’s instructions in 1:1 solution of ethanol:PBS immediately prior to injection.
Burrack A.L., Spartz E.J., Raynor J.F., Wang I., Olson M, & Stromnes I.M. (2019). Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell reports, 28(8), 2140-2155.e6.
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4

FTY720 Treatment Regimen for Candida Infection

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For FTY720 treatments, mice were intraperitoneally injected with FTY720 (Cayman Chemical) 1 μg/g of body weight in normal saline containing 0.5% DMSO daily, starting 2 days prior to C. albicans infection. 500 μg of anti-TCRγδ (clone UC7-13D5) was administered to all animals and 500 μg of anti-CD49d (clone PS/2, BioXCell) and anti-VCAM-1 (clone M/L-2.7, BioXCell) were administered to some animals i.p. once on day −2 as previously described(10 (link)).
Whitley S.K., Li M., Kashem S.W., Hirai T., Igyártó B.Z., Knizner K., Ho J., Ferris L.K., Weaver C.T., Cua D.J., McGeachy M.J, & Kaplan D.H. (2022). Local IL-23 is required for proliferation and retention of skin-resident memory Th17 cells. Science immunology, 7(77), eabq3254.
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5

Immune Cell Depletion and Modulation for Virus Challenge

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To deplete CD11b+ cells, CD11bDTR chimeric mice are treated with 10 ng/g body weight intravenously on days
−4, −1 and 50 ng diphtheria toxin (Sigma) intravaginally on days −4, −3, −2, −1. To
deplete CD4 T cells, mice were treated with 300 µg intravenously on day −4, −1 and 10 µg anti-mouse
CD4 Ab (GK1.5, BioXCell) intravaginally for four consecutive days before virus challenge. For neutralization of IFN-γ, mice
were treated with 500 µg intravenously on day −4, −1 and 10 µg anti-mouse IFN-γ Ab
(R4–6A2, BioXCell) intravaginally for four consecutive days before virus challenge. For fluorescent antibody injection,
mice were injected intravenously with 200 µg of FITC-conjugated mouse IgG antibody (Jackson ImmunoResearch). For HSV-2
monoclonal Ab (MAb) treatment, mice were injected intravenously or intravaginally with 1 µg of mouse IgG1 MAb specific for
glycoprotein D of HSV (E317, Absolute Antibody). Thirty seconds after ivag injection or 3 hours after i.v. injection of MAb, mice
were infected intravaginally with 2500 pfu of WT HSV-2. For FTY720 treatment, immunized mice were given drinking water containing
4 µg/mL of FTY720 (Cayman Chemical) for 2 weeks. For CpG1826 treatment, mice were injected intravaginally with 100
µg of CpG1826 (TriLink BioTechnologies).
Oh J.E., Iijima N., Song E., Lu P., Klein J., Jiang R., Kleinstein S.H, & Iwasaki A. (2019). Migrant memory B cells secrete luminal antibody in the vagina. Nature, 571(7763), 122-126.
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6

FTY720 Delivery Post-Spinal Cord Injury

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FTY720 or saline (vehicle) was injected intraperitoneally 24 h after the SCI. Rats in the FTY720 group received 1.5 mg/kg of FTY720 (Cayman Chemical, Ann Arbor, MI) diluted in saline, whereas the control group received the same volume of plain saline.
Yamazaki K., Kawabori M., Seki T., Takamiya S., Tateno T., Konno K., Watanabe M, & Houkin K. (2020). FTY720 Attenuates Neuropathic Pain after Spinal Cord Injury by Decreasing Systemic and Local Inflammation in a Rat Spinal Cord Compression Model. Journal of Neurotrauma, 37(15), 1720-1728.
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7

FTY720 Dosage Optimization in Ischemia-Reperfusion

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The low-dose group was administered 0.5 mg/kg FTY720 (Cayman Chemical, Ann Arbor, MI, USA) diluted in saline, whereas the high-dose group was treated with 1.5 mg/kg FTY720. FTY720 was intraperitoneally injected immediately before reperfusion.
Wang Z., Higashikawa K., Yasui H., Kuge Y., Ohno Y., Kihara A., Midori Y.A., Houkin K, & Kawabori M. (2020). FTY720 Protects Against Ischemia–Reperfusion Injury by Preventing the Redistribution of Tight Junction Proteins and Decreases Inflammation in the Subacute Phase in an Experimental Stroke Model. Translational Stroke Research, 11(5), 1103-1116.
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8

Respiratory Allergy Model: Tracking Lung Immunity

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Naïve mice were exposed i.n. to OVA plus Alternaria once on day 0. On day 45, mice were challenged i.n. once with 10 μg OVA, and lungs were collected 6 hours later. Mice were labeled by injection of anti-mouse CD45 Ab in vivo 5 min before euthanasia, as described above. Lung homogenates were analyzed for the levels of cytokines and chemokines. Lung single-cell suspensions were analyzed by flow cytometry, as described above. In some experiments, to prevent an influx of lymphocytes into the lung tissues from circulation, mice were treated systemically by intraperitoneal (i.p.) injection of FTY720 (Cayman Chemical, Ann Arbor, MI) 30 (link) (20 μg in 100 μl PBS) for 3 days before OVA challenge; FTY720 causes internalization of the G protein-coupled sphingosine-1phosphate receptor 1 (S1PR1) and prevents lymphocyte egress from lymph nodes. 30 (link)
Kobayashi T., Iijima K., Matsumoto K., Lama J.K, & Kita H. (2023). Lung-resident CD69+ST2+ TH2 cells mediate long-term type 2 memory to inhaled antigen in mice. The Journal of allergy and clinical immunology, 152(1).
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9

Immune Cell Depletion and Modulation for Virus Challenge

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To deplete CD11b+ cells, CD11bDTR chimeric mice are treated with 10 ng/g body weight intravenously on days
−4, −1 and 50 ng diphtheria toxin (Sigma) intravaginally on days −4, −3, −2, −1. To
deplete CD4 T cells, mice were treated with 300 µg intravenously on day −4, −1 and 10 µg anti-mouse
CD4 Ab (GK1.5, BioXCell) intravaginally for four consecutive days before virus challenge. For neutralization of IFN-γ, mice
were treated with 500 µg intravenously on day −4, −1 and 10 µg anti-mouse IFN-γ Ab
(R4–6A2, BioXCell) intravaginally for four consecutive days before virus challenge. For fluorescent antibody injection,
mice were injected intravenously with 200 µg of FITC-conjugated mouse IgG antibody (Jackson ImmunoResearch). For HSV-2
monoclonal Ab (MAb) treatment, mice were injected intravenously or intravaginally with 1 µg of mouse IgG1 MAb specific for
glycoprotein D of HSV (E317, Absolute Antibody). Thirty seconds after ivag injection or 3 hours after i.v. injection of MAb, mice
were infected intravaginally with 2500 pfu of WT HSV-2. For FTY720 treatment, immunized mice were given drinking water containing
4 µg/mL of FTY720 (Cayman Chemical) for 2 weeks. For CpG1826 treatment, mice were injected intravaginally with 100
µg of CpG1826 (TriLink BioTechnologies).
Oh J.E., Iijima N., Song E., Lu P., Klein J., Jiang R., Kleinstein S.H, & Iwasaki A. (2019). Migrant memory B cells secrete luminal antibody in the vagina. Nature, 571(7763), 122-126.
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10

FTY720 Modulation of Inflammatory Responses

Cited 1 time
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For FTY720 treatment in vivo, FTY720 (10006292, 0.3 mg/kg, Cayman Chemical company, USA) was dissolved in PBS and administered intraperitoneally for 3, 10 or 30 consecutive days. For FTY720 treatment in vitro, cells were treated with 100 nM non-phosphorylated FTY720 for 1h ahead and then stimulated with LPS and IFN-γ for another 24h. The dose and duration of FTY720 was selected based on according to our preliminary experiments and previous studies13 (link)–15 (link). Stattic (10 nM) was administered to block STAT3 pathway.
Qin C., Fan W.H., Liu Q., Shang K., Murugan M., Wu L.J., Wang W, & Tian D.S. (2017). FTY720 protects against ischemic white matter damage by modulating microglia toward M2 polarization via STAT3 pathway. Stroke, 48(12), 3336-3346.
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