THP-1 macrophage-like cells, BMDM, HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE, and transferred onto a PVDF membrane. The membranes were immunoblotted with antibodies against Phospho-NF-κB p65 (3033, Cell Signaling), Phospho-IκBα (9246, Cell Signaling), IκBα (4812, Cell Signaling), Phospho-ERK (4370, Cell Signaling), ERK (4695, Cell Signaling), Phospho-JNK (4668, Cell Signaling), Phospho-AKT (4060, Cell Signaling), AKT (9272, Cell Signaling), Phospho-STAT3 (9145, Cell Signaling), Flag®M2 (F1804, Sigma-Aldrich), SARS-CoV-2 S (GTX632605, GeneTex) and β-actin (A2228, Sigma). Immunoreactive protein bands were detected using ECL super signal west femto substrate reagent (Thermo Scientific).
Sars cov 2 s
Manufactured by GeneTex
The SARS-CoV-2 S is a recombinant protein that represents the spike (S) protein of the SARS-CoV-2 virus. The S protein is a key structural component of the virus and plays a crucial role in its attachment and entry into host cells.
Automatically generated - may contain errors
Lab products found in correlation
Complete protease inhibitor and phosphatase inhibitor cocktail, by Roche (2 mentions)
Ecl super signal west femto substrate reagent, by Thermo Fisher Scientific (2 mentions)
Phospho jnk, by Cell Signaling Technology (2 mentions)
Phospho erk, by Cell Signaling Technology (2 mentions)
Phospho iκbα, by Cell Signaling Technology (2 mentions)
Phospho stat3, by Cell Signaling Technology (2 mentions)
Phospho akt, by Cell Signaling Technology (2 mentions)
Phospho nf κb p65, by Cell Signaling Technology (2 mentions)
β actin, by Merck Group (2 mentions)
Flag m2, by Merck Group (1 mentions)
2 protocols using sars cov 2 s
1
Immunoblotting Analysis of Signaling Pathways
2
Western Blot Analysis of Immune Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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THP1 cells, BMDM, HEK293T, A549, and Calu3 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE, and transferred onto a PVDF membrane. The membranes were immunoblotted with antibodies against Phospho-NF-κB p65 (3033, Cell Signaling), Phospho-IκBα (9246, Cell Signaling), IκBα (4812, Cell Signaling), Phospho-ERK (4370, Cell Signaling), ERK (4695, Cell Signaling), Phospho-JNK (4668, Cell Signaling), Phospho-AKT (4060, Cell Signaling), Phospho-STAT3 (9145, Cell Signaling), SARS-CoV-2 S (GTX632604, GeneTex), and β-actin (A2228, Sigma-Aldrich). Immunoreactive protein bands were detected using ECL super signal west femto substrate reagent (Thermo Fisher Scientific).
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