RNeasy mini kit (74104), RT2 SYBRR Green qPCR master mix (330502), human primer β-actin (PPH00073G-200), and STAT3 (PPH00708F-200) were purchased from Qiagen;
Superscript R III first-strand synthesis system for RT-PCR (18080-051) was purchased from Invitrogen-Life Technology; selection antibiotic Puromycin dihydrochloride (P-8833) was purchased from Sigma and Blasticidin S hydrochloride (A4166) was purchased from Biometric. Primary antibodies anti-STAT3 (9139) and phospho-STAT3/Tyr705 (9145) were obtained from Cell Signalling Technology; phospho-STAT3/S727 (ab32143) was obtained from Abcam, β actin mouse monoclonal (A2228) was obtained from Sigma; Inhibitor of STAT3, Sttatic (BML-EI368) was purchased from Enzo Life Sciences.
Reagents for routine techniques were purchased from Bio-Rad laboratories, Thermo Fisher Scientific and Sigma Aldrich.
Superscript R III first-strand synthesis system for RT-PCR (18080-051) was purchased from Invitrogen-Life Technology; selection antibiotic Puromycin dihydrochloride (P-8833) was purchased from Sigma and Blasticidin S hydrochloride (A4166) was purchased from Biometric. Primary antibodies anti-STAT3 (9139) and phospho-STAT3/Tyr705 (9145) were obtained from Cell Signalling Technology; phospho-STAT3/S727 (ab32143) was obtained from Abcam, β actin mouse monoclonal (A2228) was obtained from Sigma; Inhibitor of STAT3, Sttatic (BML-EI368) was purchased from Enzo Life Sciences.
Reagents for routine techniques were purchased from Bio-Rad laboratories, Thermo Fisher Scientific and Sigma Aldrich.