Log In Sign up
The largest database of trusted experimental protocols

Nicolet is5 ft ir spectrophotometer

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States, United Kingdom

The Nicolet iS5 FT-IR spectrophotometer is a compact and versatile instrument designed for infrared spectroscopy analysis. It utilizes Fourier transform infrared (FT-IR) technology to measure the absorption and transmission of infrared light by samples, providing information about the molecular composition and structure of materials.

Automatically generated - may contain errors

Lab products found in correlation

7 tesla solarix ftms system, by Bruker (4 mentions) Gf254 plates, by Qingdao Haiyang Chemical (3 mentions) L 2130 pump, by Hitachi (2 mentions) L 2130, by Hitachi (2 mentions) Ecz nmr spectrometer, by JEOL (2 mentions) Rp 18w uv254, by Macherey-Nagel (2 mentions) Silica gel, by Merck Group (2 mentions) Kieselgel 60 f254, by Merck Group (2 mentions) L 2455, by Hitachi (2 mentions) Id7 atr, by Thermo Fisher Scientific (2 mentions) Luna 5 m c18 2 100, by Phenomenex (1 mentions) Agilent cary 60 uv vis spectrophotometer, by Agilent Technologies (1 mentions) Axis ultra dld spectrometer, by Shimadzu (1 mentions) Vario el cube elemental analyzer, by Elementar (1 mentions) Lab ram hr evolution instrument, by Horiba (1 mentions) 1200 hplc system, by Agilent Technologies (1 mentions) Avance 300, by Bruker (1 mentions) Organic solvents, by Merck Group (1 mentions) Topspin 3, by Bruker (1 mentions) Autopol 4 automatic, by Rudolph Research Analytical (1 mentions)

24 protocols using nicolet is5 ft ir spectrophotometer

1

Characterization of Developed Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols
The presence of characteristic groups in the structure of the developed materials was determined using Fourier transform infrared (FT-IR) spectroscopy. For this purpose, a Thermo Scientific Nicolet iS5 FT-IR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an iD7 ATR was used. FT-IR spectra were recorded within the 4000–400 cm−1 wavelength range at room temperature.
Kędzierska M., Drabczyk A., Jamroży M., Kudłacik-Kramarczyk S., Głąb M., Potemski P, & Tyliszczak B. (2022). Iron Oxide Magnetic Nanoparticles with a Shell Made from Nanosilver—Synthesis Methodology and Characterization of Physicochemical and Biological Properties. Materials, 15(12), 4050.
+ Open protocol
+ Expand
2

Spectroscopic Characterization of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
The specific rotation values and IR spectra were measured using a JASCO P-2000 digital polarimeter and a THERMO Scientific Nicolet iS5 FT-IR spectrophotometer, respectively. ESIMS and HRESIMS were recorded using a BRUKER 7 Tesla solariX FTMS system. NMR spectra were obtained from a JEOL Resonance ECZ 400S or an ECZ 600R NMR spectrometer, with the residual signals of CHCl3 (δH 7.26 ppm) and CDCl3 (δC 77.0 ppm) used as the internal standards for 1H and 13C NMR, respectively. Coupling constants (J) are provided in Hz. Column chromatography was carried out with a silica gel (230~400 mesh, MERCK) column. Thin-layer chromatography was performed on plates precoated with silica gel 60 F254 (0.25-mm-thick, MERCK); the plates then sprayed with 10% (v/v) H2SO4 in methanol, followed by heating to visualize the spots. A normal-phase (NP) HPLC was performed using a system comprised of a HITACHI 5110 pump, a RHEODYNE 7725i injection port and a NP column (YMC pack SIL, 5 μm, 12 nm, 250 × 20 mm, YMC group). Reverse-phase (RP) HPLC was performed using a system comprised of a HITACHI L-2130 pump, a HITACHI L-2455 photodiode array detector, a RHEODYNE 7725i injection port and a RP column (Luna 5 µm C18(2) 100 Å, 250 × 21.2 mm, Phenomenex).
Huynh T.H., Liu C.J., Liu Y.H., Chien S.Y., Wen Z.H., Fang L.S., Chen J.J., Wu Y.C., Su J.H, & Sung P.J. (2023). Briavioids E–G, Newly Isolated Briarane-Diterpenoids from a Cultured Octocoral Briareum violaceum. Marine Drugs, 21(2), 124.
+ Open protocol
+ Expand
3

Characterizing Hydrogel Structure via FT-IR

Check if the same lab product or an alternative is used in the 5 most similar protocols
The next analysis included verifying the presence of characteristic functional groups in the structure of hydrogels using FT-IR spectroscopy. This study was used also to verify whether the incubation of hydrogels in simulated physiological liquids affected their structure; therefore, the samples were subjected to the analysis before and after the incubation. The study was performed using a Thermo Scientific Nicolet iS5 FT-IR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). FT-IR spectra of hydrogel samples were recorded within the wavenumber range 4000–500 cm−1 (32 scans and the resolution 4.0 cm−1).
Kędzierska M., Bańkosz M, & Potemski P. (2022). Studies on the Impact of the Photoinitiator Amount Used during the PVP-Based Hydrogels’ Synthesis on Their Physicochemical Properties. Materials, 15(17), 6089.
+ Open protocol
+ Expand
4

Spectral Analysis of Modified Epoxy Resins

Check if the same lab product or an alternative is used in the 5 most similar protocols
0.5–1 mg of unmodified epoxy resin, lysine, BCN and BCN-modified epoxy resin were mixed with KBr in an agate mortar and ground for 1 min separately to obtain the test sample for tableting, respectively. The FT-IR spectra of the samples were recorded using a Thermo Nicolet iS5 FT-IR spectrophotometer from 4000 to 500 cm−1.
Li H., Yin Y., Wang A., Li N., Wang R., Zhang J., Chen X., Pei X, & Xie T. (2020). Stable immobilization of aldehyde ketone reductase mutants containing nonstandard amino acids on an epoxy resin via strain-promoted alkyne–azide cycloaddition. RSC Advances, 10(5), 2624-2633.
+ Open protocol
+ Expand
5

FT-IR Analysis of Composite Degradation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The FT-IR test of the composites before and after degradation was carried out by using a Thermo Scientific Nicolet iS 5 FTIR spectrophotometer in transmission mode.
Wu L., Liu J., Liu Y., Huang R., Tang N., Wang X, & Hu L. (2021). In situ growth of Fe3O4 on montmorillonite surface and its removal of anionic pollutants. RSC Advances, 11(53), 33399-33407.
+ Open protocol
+ Expand
6

Comprehensive Characterization of Porous Carbon

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Zeiss Merlin scanning electron microscopy (SEM, Zeiss, Jena, Germany) was used for characterizing the surface morphologies of samples. N2 adsorption/desorption isotherms were measured at liquid nitrogen temperature using a Quantachrome Autosorb-iQ gas sorption analyzer (Quantachrome, Boynton Beach, FL, USA). Before the measurements, the porous carbon sample with a mass of approximately 100 mg was pretreated at 150 °C for 4 h under vacuum condition. A Nicolet iS5 FT-IR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used for recording the IR spectra. An Axis Ultra DLD spectrometer (Kratos Analytical, Manchester, UK) was applied for performing the XPS measurements. A LabRAM HR Evolution instrument (Horiba, Longjumeau, France) was applied for measuring the Raman spectra. The optical adsorptions of RhB and CAP solutions were measured on an Agilent Cary60 UV-Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). Element analysis of C, H, N, S, and O was performed on a vario EL Cube elemental analyzer (Elementar Analysensysteme GmbH, Langenselbold, Germany).
Liang D., Tian X., Zhang Y., Zhu G., Gao Q., Liu J, & Yu X. (2022). A Weed-Derived Hierarchical Porous Carbon with a Large Specific Surface Area for Efficient Dye and Antibiotic Removal. International Journal of Molecular Sciences, 23(11), 6146.
+ Open protocol
+ Expand
7

Instrumental Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optical rotation values were measured using a JASCO P-1010 digital polarimeter. IR spectra were obtained with a Thermo Scientific Nicolet iS5 FT-IR spectrophotometer. NMR spectra were recorded on a 400 MHz Jeol ECZ NMR spectrometer using the residual CHCl3 (δH 7.26 ppm) and CDCl3 signals (δC 77.0 ppm) as internal standards for 1H and 13C NMR, respectively; coupling constants (J) are presented in Hz. ESIMS and HRESIMS were recorded using a Bruker 7 Tesla solariX FTMS system. Column chromatography was carried out with silica gel (230–400 mesh, Merck). TLC was performed on plates precoated with silica gel 60 F254 (Merck) and RP-18W/UV254 (0.15 mm-thick, Macherey-Nagel), then sprayed with 20% H2SO4 solution followed by heating to visualize the spots. NP-HPLC was performed using a system comprised of a Hitachi L-5110 pump and a Rheodyne 7725i injection port with a normal-phase column (Galaksil® EF-SiO2, 5 μm 120 Å, S/N E08210401; Galak Co., Wuxi, CN). RP-HPLC was performed using a system comprised of a Hitachi L-2130 pump, a Hitachi L-2455 photodiode array detector, and a Rheodyne 7725i injection port with a reverse-phase column (Supelco, Ascentis® C18, 581343-U, 250 mm × 10 mm, 5 μm).
Phan G.H., Hu H.C., Chang F.R., Wen Z.H., Chen J.J., Chung H.M., Tsai Y.C, & Sung P.J. (2022). Norsesquiterpenoids from the octocoral Paralemnalia thyrsoides (Ehrenberg 1834). RSC Advances, 12(43), 27970-27976.
+ Open protocol
+ Expand
8

FT-IR Analysis of Mucilage Functional Groups

Check if the same lab product or an alternative is used in the 5 most similar protocols
Functional groups of the mucilage were determined by FT-IR spectroscopy. The Nicolet iS5 FT-IR spectrophotometer (Thermo Fisher Scientific) was equipped with an iD5 ATR accessory featuring a diamond crystal cell. FT-IR spectra were recorded over a wavelength range 3600–800 cm−1 at a resolution of 2 cm−1, with 16 scans per spectrum.
Oh S, & Kim D.Y. (2022). Characterization, Antioxidant Activities, and Functional Properties of Mucilage Extracted from Corchorus olitorius L.. Polymers, 14(12), 2488.
+ Open protocol
+ Expand
9

Spectroscopic Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optical rotation values were measured using a JASCO P-1010 digital polarimeter. IR spectra were obtained with a Thermo Scientific Nicolet iS5 FT-IR spectrophotometer. NMR spectra were recorded on a 400 MHz Jeol ECZ NMR spectrometer using the residual CHCl3 (δH 7.26 ppm) and CDCl3 signals (δC 77.0 ppm) as internal standards for 1H and 13C NMR, respectively; coupling constants (J) are presented in Hz. ESIMS and HRESIMS were recorded using a Bruker 7 Tesla solariX FTMS system. Column chromatography was carried out with silica gel (230–400 mesh, Merck). TLC was performed on plates precoated with silica gel 60 F254 (Merck) and RP-18W/UV254 (0.15 mm-thick, Macherey-Nagel), then sprayed with 10% H2SO4 solution followed by heating to visualize the spots.
Tseng H.J., Kuo L.M., Tsai Y.C., Hu H.C., Chen P.J., Chien S.Y., Sheu J.H, & Sung P.J. (2023). Sinulariaone A: a novel diterpenoid with a 13-membered carbocyclic skeleton from an octocoral Sinularia species. RSC Advances, 13(15), 10408-10413.
+ Open protocol
+ Expand
10

Synthesis and characterization of organic compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
All solvents and chemical reagents were purchased from commercial sources and used directly without further purification. The progress of all reactions was monitored by analytical thin-layer chromatography on silica gel 60 F254 plates (Merck). The purification by column chromatography was performed using 200-300 mesh silica gel (Qingdao Haiyang Chemical Co. Ltd.). 1H-NMR spectra were recorded on a Bruker AVANCE 300 or 500 MHz spectrometer in DMSO-d6. 13C-NMR spectra were recorded on a Bruker AVANCE 75 or 125 MHz spectrometer in DMSO-d6. Chemical shift values were reported in δ values parts per million (ppm), referenced to dimethyl sulfoxide (DMSO) using the residual solvent signal as an internal reference. High-resolution mass spectrometry data (HRMS) were recorded on the ICR (Fourier transform ion cyclotron resonance, FTICR) analyzer using an ESI source. Infrared spectra (IR) were recorded on a Thermo Scientific Nicolet iS5 FT-IR spectrophotometer and reported as wavelength numbers (cm−1). Melting points were determined with an X-4A microscopic melting point apparatus, which was uncorrected. All of the test compounds exhibited >95% purity as determined by HPLC. HPLC analysis was performed using an Agilent HPLC 1200 system (column, Agilent C18, 4.6 mm × 150 mm, 5 μm).
Zhang G., Wang F., Li S., Cheng K.W., Zhu Y., Huo R., Abdukirim E., Kang G, & Chou T.F. (2022). Discovery of small-molecule inhibitors of RUVBL1/2 ATPase. Bioorganic & medicinal chemistry, 62, 116726.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!